About: Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes

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About: Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes
Creator	Zhang, Yi Liu, Lihong Wang, Pengfei Shi, Wei Wang, Lingshu Zhou, Tongqing Tsybovsky, Yaroslav Misasi, John Sullivan, Nancy Zhang, Baoshan Mascola, John Teng, I-Ting Corbett, Kizzmekia Graham, Barney Kwong, Peter Banach, Bailey Ho, David Olia, Adam Cerutti, Gabriele Chang, Lauren Dipiazza, Anthony Gorman, Jason Phung, Emily Ruckwardt, Tracy Shapiro, Lawrence Wang, Shuishu Yin, Michael Abiona, Olubukula Dekosky, Brandon Fahad, Ahmed Gov (, Pdkwong@nih Katsamba, Phinikoula Lopez Acevedo, Sheila Madan, Bharat Nazzari, Alexandra Oliveira De Souza, Matheus Pan, Xiaoli Petrova, Yuliya Wolfe, Jacy
Source	BioRxiv; Medline
abstract	Biotin-labeled molecular probes, comprising specific regions of the SARS-CoV-2 spike, would be helpful in the isolation and characterization of antibodies targeting this recently emerged pathogen. To develop such probes, we designed constructs incorporating an N-terminal purification tag, a site-specific protease-cleavage site, the probe region of interest, and a C-terminal sequence targeted by biotin ligase. Probe regions included full-length spike ectodomain as well as various subregions, and we also designed mutants to eliminate recognition of the ACE2 receptor. Yields of biotin-labeled probes from transient transfection ranged from ∼0.5 mg/L for the complete ectodomain to >5 mg/L for several subregions. Probes were characterized for antigenicity and ACE2 recognition, and the structure of the spike ectodomain probe was determined by cryo-electron microscopy. We also characterized antibody-binding specificities and cell-sorting capabilities of the biotinylated probes. Altogether, structure-based design coupled to efficient purification and biotinylation processes can thus enable streamlined development of SARS-CoV-2 spike-ectodomain probes.
has issue date	2020-06-23(xsd:dateTime)
bibo:doi	10.1101/2020.06.22.166033
bibo:pmid	32596696
has license	biorxiv
sha1sum (hex)	15c0d24966e49379a362db295a2ca248e866f1d8
schema:url	https://doi.org/10.1101/2020.06.22.166033
resource representing a document's title	Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes
has PubMed identifier	32596696
schema:publication	bioRxiv
resource representing a document's body	covid:15c0d24966e49379a362db295a2ca248e866f1d8#body_text
is schema:about of	named entity 'probes' named entity 'coupled' named entity 'biotin' named entity 'purification' named entity 'biotinylated' named entity 'CHARACTERIZED' named entity 'RECEPTOR' named entity 'BINDING' covid:arg/15c0d24966e49379a362db295a2ca248e866f1d8 named entity 'constructs' named entity 'isolation' named entity 'transient' named entity 'molecular probes' named entity 'subregions' named entity '3D reconstruction' named entity 'molecular probes' named entity 'biotin' named entity 'Wrapp' named entity 'Receptor' named entity 'size-exclusion chromatography' named entity 'biotinylation' named entity 'antibodies' named entity 'antibodies' named entity 'biotin' named entity 'RBD' named entity 'ACE2' named entity 'RBD' named entity 'CCD camera' named entity 'dipeptide' named entity 'Yeast display' named entity 'ectodomain' named entity 'therapeutic antibodies' named entity 'biotinylation' named entity 'G502' named entity 'ACE2' named entity 'Env' named entity 'NaCl' named entity 'biosensor' named entity 'LysC' named entity 'yeast' named entity 'plasmids' named entity 'yeast' named entity 'antigen' named entity '22 nm'

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