About: Experimental inoculation of adult dairy cows with bovine coronavirus and detection of coronavirus in feces by RT-PCR

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About: Experimental inoculation of adult dairy cows with bovine coronavirus and detection of coronavirus in feces by RT-PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Experimental inoculation of adult dairy cows with bovine coronavirus and detection of coronavirus in feces by RT-PCR
Creator	Tsunemitsu, H Saif, L Smith, D
topic	covid:15c7d575fdc941daae3f172bf153526bd018474e#this
Source	Medline; PMC
abstract	A reverse transcriptase PCR (RT-PCR) targeting a 407 bp fragment of the nucleocapsid gene of bovine coronavirus (BCV) was developed for detection of BCV RNA in feces of experimentally inoculated cattle. The sensitivity and specificity of the RT-PCR were confirmed using tissue culture-adapted BCV strains and feces of 2 calves inoculated with BCV. Ten nonpregant, BCV seropositive, adult dairy cows were inoculated with winter dysentery (WD) (n = 8) or calf diarrhea (CD) (n = 2) strains of BCV intranasally and orally (n = 2) or through a surgically-placed duodenal catheter (n = 8) with and without dexamethasone treatment or feeding ice water. The 6 cows inoculated with BCV intranasally and through a duodenal catheter (2 of 2 cows given CD BCV and 4 of 6 cows given WD BCV) developed mild diarrhea, and BCV was detected in diarrheal feces by RT-PCR, ELISA or immune electron microscopy. These results suggest that CD and WD strains of BCV can cause diarrhea in adult cows in conjunction with host or environmental factors and that RT-PCR might be useful to diagnose BCV infections in calves and adult cows.
has issue date	2014-04-08(xsd:dateTime)
bibo:doi	10.1007/s007050050493
bibo:pmid	10076517
has license	no-cc
sha1sum (hex)	15c7d575fdc941daae3f172bf153526bd018474e
schema:url	https://doi.org/10.1007/s007050050493
resource representing a document's title	Experimental inoculation of adult dairy cows with bovine coronavirus and detection of coronavirus in feces by RT-PCR
has PubMed Central identifier	PMC7086631
has PubMed identifier	10076517
schema:publication	Arch Virol
resource representing a document's body	covid:15c7d575fdc941daae3f172bf153526bd018474e#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/15c7d575fdc941daae3f172bf153526bd018474e
is schema:about of	named entity 'ADULT' named entity 'DAIRY' named entity 'CALVES' named entity 'ICE' named entity 'DIAGNOSE' named entity 'PLACED' named entity 'CATHETER' named entity 'FECES' named entity 'NEONATAL CALF DIARRHEA' named entity 'ADDRESS' named entity 'SUGGEST' named entity 'ISOLATED' named entity 'RNA' named entity 'NUCLEOCAPSID' named entity 'PRESENT' named entity 'STUDY' named entity 'TREATMENT' named entity 'IMPORTANT' named entity 'detected' named entity 'address' named entity 'calf' named entity 'cows' named entity 'Ten' named entity 'inoculation' named entity 'BRIEF' named entity 'DETECTION' named entity 'BCV' named entity 'CATTLE' named entity 'CONJUNCTION' named entity 'THESE' named entity 'WATER' covid:arg/15c7d575fdc941daae3f172bf153526bd018474e named entity 'INOCULATION' named entity 'CORONAVIRUS' named entity 'BOVINE CORONAVIRUS' named entity 'HOST' named entity 'DETECTED' named entity 'REVERSE TRANSCRIPTASE PCR' named entity 'DYSENTERY' named entity 'GIVEN' named entity 'MIGHT BE' named entity 'STRAINS' named entity 'DUODENAL' named entity 'USEFUL' named entity 'DEXAMETHASONE' named entity 'PCR' named entity 'DIARRHEA' named entity 'INFECTIONS' named entity 'COWS' named entity 'FECES' named entity 'REPORT' named entity 'EXPERIMENTAL' named entity 'ENVIRONMENTAL FACTORS' named entity 'CALF' named entity 'DAIRY' named entity 'ADULT' named entity 'BOVINE CORONAVIRUS' named entity 'DETECTION' named entity 'RESULTS' named entity 'TARGETING' named entity 'CAUSE' named entity 'ORALLY' named entity 'ACUTE DIARRHEA' named entity 'RECENT' named entity 'VIRUS' named entity 'FEEDING' named entity 'RT-PCR' named entity 'OUTBREAKS' named entity 'RT-PCR' named entity '282' named entity 'TISSUE CULTURE' named entity 'FRAGMENT OF' named entity 'PATHOGEN' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'WINTER' named entity 'COWS' named entity 'GENE'

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