About: Evaluation and Clinical Validation of Two Field–Deployable Reverse Transcription-Insulated Isothermal PCR Assays for the Detection of the Middle East Respiratory Syndrome

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About: Evaluation and Clinical Validation of Two Field–Deployable Reverse Transcription-Insulated Isothermal PCR Assays for the Detection of the Middle East Respiratory Syndrome–Coronavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation and Clinical Validation of Two Field–Deployable Reverse Transcription-Insulated Isothermal PCR Assays for the Detection of the Middle East Respiratory Syndrome–Coronavirus
Creator	Tsai, Yun-Long Cheon, Shinhye Kim, Meehyein Kim, Yeon-Sook Lin, Yu-Chun Nam, Sangwoo Park, Hyun Lee, Pei-Yu Balasuriya, Udeni Cho, Nam Go, Yun Wang, Hwa-Tang Ku, Keun
Source	Elsevier; Medline; PMC
abstract	Middle East respiratory syndrome (MERS) is an emerging zoonotic viral respiratory disease that was first identified in Saudi Arabia in 2012. In 2015, the largest MERS outbreak outside of the Middle East region occurred in the Republic of Korea. The rapid nosocomial transmission of MERS-coronavirus (MERS-CoV) in Korean health care settings highlighted the importance and urgent need for a rapid and reliable on-site diagnostic assay to implement effective control and preventive measures. Here, the evaluation and validation of two newly developed reverse transcription–insulated isothermal PCR (RT-iiPCR) methods targeting the ORF1a and upE genes of MERS-CoV are described. Compared with World Health Organization-recommended singleplex real-time quantitative RT-PCR (RT-qPCR) assays, both RT-iiPCR assays had comparable analytical sensitivity for the detection of MERS-CoV RNA in tissue culture fluid and in sputum samples spiked with infectious virus. Furthermore, clinical evaluation was performed with sputum samples collected from subjects with acute and chronic respiratory illnesses, including patients infected with MERS-CoV. The overall agreement values between the two RT-iiPCR assays and the reference RT-qPCR assays were 98.06% (95% CI, 94.43%–100%; κ = 0.96) and 99.03% (95% CI, 95.88%–100%; κ = 0.99) for ORF1a and upE assays, respectively. The ORF1a and upE MERS-CoV RT-iiPCR assays coupled with a field-deployable system provide a platform for a highly sensitive and specific on-site tool for diagnosis of MERS-CoV infections.
has issue date	2017-08-12(xsd:dateTime)
bibo:doi	10.1016/j.jmoldx.2017.06.007
bibo:pmid	28807812
has license	no-cc
sha1sum (hex)	162b902c4576787077112f6bdb1e4e5bc7b93d1f
schema:url	https://doi.org/10.1016/j.jmoldx.2017.06.007
resource representing a document's title	Evaluation and Clinical Validation of Two Field–Deployable Reverse Transcription-Insulated Isothermal PCR Assays for the Detection of the Middle East Respiratory Syndrome–Coronavirus
has PubMed Central identifier	PMC7106309
has PubMed identifier	28807812
schema:publication	J Mol Diagn
resource representing a document's body	covid:162b902c4576787077112f6bdb1e4e5bc7b93d1f#body_text
is schema:about of	named entity 'MIDDLE EAST' named entity 'control' named entity 'sputum' named entity 'Saudi Arabia' named entity 'MERS-CoV' named entity 'sputum' named entity 'PCR' named entity 'TARGETING' named entity 'REFERENCE' named entity 'GENES' named entity 'DETECTION' named entity 'REPUBLIC OF KOREA' named entity 'VALUES' named entity 'TISSUE CULTURE' named entity 'RAPID' named entity 'RELIABLE' named entity 'SAUDI ARABIA' named entity 'UPE' named entity 'SPUTUM' named entity 'CLINICAL EVALUATION' named entity 'HIGHLY SENSITIVE' named entity 'HEALTH CARE' named entity 'SUBJECTS' named entity 'COMPARED' named entity 'NEWLY' named entity 'IDENTIFIED' named entity 'TOOL' named entity 'MIDDLE EAST RESPIRATORY SYNDROME ' named entity '2015' named entity 'MIDDLE EAST' covid:arg/162b902c4576787077112f6bdb1e4e5bc7b93d1f named entity 'PATIENTS' named entity 'PLATFORM' named entity 'REVERSE TRANSCRIPTION' named entity 'MERS' named entity 'MOL' named entity 'ILLNESSES' named entity 'RESPIRATORY' named entity 'DETECTION' named entity 'CLINICAL VALIDATION' named entity 'EVALUATION' named entity 'MERS-COV' named entity 'NEED FOR' named entity 'INFECTED' named entity 'SENSITIVITY' named entity '0.99' named entity 'OUTBREAK' named entity 'EFFECTIVE' named entity 'SAMPLES' named entity 'PERFORMED' named entity 'AGREEMENT' named entity 'FOR DIAGNOSIS' named entity 'CONTROL' named entity 'PCR' named entity 'INCLUDING' named entity 'METHODS' named entity 'PROVIDE' named entity 'NOSOCOMIAL TRANSMISSION' named entity 'WORLD HEALTH' named entity 'REAL-TIME QUANTITATIVE RT-PCR' named entity 'RT-QPCR' named entity 'CHRONIC' named entity 'HERE' named entity 'COUPLED' named entity 'MERS-COV RNA' named entity 'SETTINGS' named entity 'SITE' named entity 'CULTURE FLUID' named entity 'HTTP'

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