About: The SARS-CoV-2 cytopathic effect is blocked with autophagy modulators

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The SARS-CoV-2 cytopathic effect is blocked with autophagy modulators
Creator	Klumpp-Thomas, Carleen Hu, Xin Zheng, Wei Rasmussen, Lynn Zhu, Wei Huang, Wenwei Shinn, Paul Simeonov, Anton Xu, Miao Bostwick, Robert Hall, Matthew Chen, Catherine Shen, Min Gorshkov, Kirill Gov, -Kirill Itkin, Zina Michael, Sam Pradhan, Manisha Lo, Donald Shamim, Khalida Tran, Bruce
Source	BioRxiv; Medline; PMC
abstract	SARS-CoV-02 is a new type of coronavirus capable of rapid transmission and causing severe clinical symptoms; much of which has unknown biological etiology. It has prompted researchers to rapidly mobilize their efforts towards identifying and developing anti-viral therapeutics and vaccines. Discovering and understanding the virus’ pathways of infection, host-protein interactions, and cytopathic effects will greatly aid in the design of new therapeutics to treat COVID-19. While it is known that chloroquine and hydroxychloroquine, extensively explored as clinical agents for COVID-19, have multiple cellular effects including inhibiting autophagy, there are also dose-limiting toxicities in patients that make clearly establishing their potential mechanisms-of-action problematic. Therefore, we evaluated a range of other autophagy modulators to identify an alternative autophagy-based drug repurposing opportunity. In this work, we found that 6 of these compounds blocked the cytopathic effect of SARS-CoV-2 in Vero-E6 cells with EC(50) values ranging from 2.0 to 13 μM and selectivity indices ranging from 1.5 to >10-fold. Immunofluorescence staining for LC3B and LysoTracker dye staining assays in several cell lines indicated their potency and efficacy for inhibiting autophagy correlated with the measurements in the SARS-CoV-2 cytopathic effect assay. Our data suggest that autophagy pathways could be targeted to combat SARS-CoV-2 infections and become an important component of drug combination therapies to improve the treatment outcomes for COVID-19.
has issue date	2020-05-28(xsd:dateTime)
bibo:doi	10.1101/2020.05.16.091520
bibo:pmid	32511355
has license	cc-by-nc-nd
sha1sum (hex)	169d68574fef39c303abaf8938c2fc4df4d6364e
schema:url	https://doi.org/10.1101/2020.05.16.091520
resource representing a document's title	The SARS-CoV-2 cytopathic effect is blocked with autophagy modulators
has PubMed Central identifier	PMC7259466
has PubMed identifier	32511355
schema:publication	bioRxiv
resource representing a document's body	covid:169d68574fef39c303abaf8938c2fc4df4d6364e#body_text
is schema:about of	named entity 'evaluated' named entity 'interactions' named entity 'SARS-CoV-2' named entity 'combination therapies' named entity 'dye' named entity 'infections' named entity 'inhibiting' named entity 'drug repurposing' named entity 'autophagy' named entity 'chloroquine' named entity 'coronavirus' named entity 'ranging' named entity 'staining' named entity 'COVID-19' named entity 'extensively' named entity 'improve' named entity 'potency' named entity 'opportunity' named entity 'hydroxychloroquine' named entity 'cytopathic' named entity 'therapeutics' named entity 'combat' named entity 'Immunofluorescence' named entity 'staining' named entity 'combination therapies' named entity 'autophagy' named entity 'SARS-CoV-2' named entity 'cytopathic effects' named entity 'COVID' named entity 'cytopathic effect' named entity 'staining' named entity 'aid' named entity 'Huh-7' named entity 'viral infection' named entity 'bioRxiv' named entity 'ImageJ' named entity 'NIH' named entity 'autophagy' named entity 'cell line' named entity 'HCQ' named entity 'autophagy' named entity 'COVID' named entity 'membrane vesicles' named entity 'DMEM' named entity 'drug toxicity' named entity 'Autophagy' named entity 'panic disorder' named entity 'Drosophila' named entity 'endocytic pathway' named entity 'peer review' named entity 'high-throughput screening' named entity 'follow-up' named entity 'endosome' named entity 'viruses' named entity 'HCQ' named entity 'protein' named entity 'negative control' named entity 'renal damage' named entity 'Caco-2' named entity 'autophagy' named entity 'EV71'

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