About: Ezrin Interacts with the SARS Coronavirus Spike Protein and Restrains Infection at the Entry Stage

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About: Ezrin Interacts with the SARS Coronavirus Spike Protein and Restrains Infection at the Entry Stage Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Ezrin Interacts with the SARS Coronavirus Spike Protein and Restrains Infection at the Entry Stage
Creator	Bruzzone, Roberto Jaume, Martial Cheung, Chung-Yan Altmeyer, Ralf Kien, François Chan, Wing-Lim Siu, Yu-Lam Millet, Jean Leung, Hiu-Lan Li, Huiying Atrice Nal, Bé Peiris, Joseph
Source	Medline; PMC
abstract	BACKGROUND: Entry of Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) and its envelope fusion with host cell membrane are controlled by a series of complex molecular mechanisms, largely dependent on the viral envelope glycoprotein Spike (S). There are still many unknowns on the implication of cellular factors that regulate the entry process. METHODOLOGY/PRINCIPAL FINDINGS: We performed a yeast two-hybrid screen using as bait the carboxy-terminal endodomain of S, which faces the cytosol during and after opening of the fusion pore at early stages of the virus life cycle. Here we show that the ezrin membrane-actin linker interacts with S endodomain through the F1 lobe of its FERM domain and that both the eight carboxy-terminal amino-acids and a membrane-proximal cysteine cluster of S endodomain are important for this interaction in vitro. Interestingly, we found that ezrin is present at the site of entry of S-pseudotyped lentiviral particles in Vero E6 cells. Targeting ezrin function by small interfering RNA increased S-mediated entry of pseudotyped particles in epithelial cells. Furthermore, deletion of the eight carboxy-terminal amino acids of S enhanced S-pseudotyped particles infection. Expression of the ezrin dominant negative FERM domain enhanced cell susceptibility to infection by SARS-CoV and S-pseudotyped particles and potentiated S-dependent membrane fusion. CONCLUSIONS/SIGNIFICANCE: Ezrin interacts with SARS-CoV S endodomain and limits virus entry and fusion. Our data present a novel mechanism involving a cellular factor in the regulation of S-dependent early events of infection.
has issue date	2012-11-21(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0049566
bibo:pmid	23185364
has license	cc-by
sha1sum (hex)	17752cd197628ca49a7b800528bdd68532e4a148
schema:url	https://doi.org/10.1371/journal.pone.0049566
resource representing a document's title	Ezrin Interacts with the SARS Coronavirus Spike Protein and Restrains Infection at the Entry Stage
has PubMed Central identifier	PMC3504146
has PubMed identifier	23185364
schema:publication	PLoS One
resource representing a document's body	covid:17752cd197628ca49a7b800528bdd68532e4a148#body_text
is schema:about of	named entity 'SPIKE PROTEIN' named entity 'SARS CORONAVIRUS' named entity 'ENVELOPE GLYCOPROTEIN' named entity 'INFECTION' named entity 'MECHANISMS' named entity 'UNKNOWNS' covid:arg/17752cd197628ca49a7b800528bdd68532e4a148 named entity 'LARGELY' named entity 'CONTROLLED BY' named entity 'REGULATE' named entity '28S' named entity 'ENTRY' named entity 'EZRIN' named entity 'DEPENDENT' named entity 'SERIES' named entity 'PROCESS' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'CELLULAR' named entity 'HOST CELL MEMBRANE' named entity 'BACKGROUND' named entity 'STAGE' named entity 'PROTEIN ' named entity 'ITS' named entity 'ENVELOPE' named entity 'FUSION' named entity 'VIRAL ENVELOPE' named entity 'COMPLEX' named entity 'ENTRY' named entity 'MOLECULAR' named entity 'SPIKE' named entity 'Vero E6' named entity 'palmitoylated' named entity 'petri dishes' named entity 'polyclonal' named entity 'ezrin' named entity 'lamellipodia' named entity 'yeast' named entity 'SARS-CoV' named entity 'SARS-CoV' named entity 'cysteine' named entity 'GFP' named entity 'C-terminal' named entity 'GST' named entity 'Biotech' named entity 'cloning' named entity 'ezrin' named entity 'SARS-CoV' named entity 'SARS-CoV' named entity 'cell fusion' named entity 'Molecular Devices' named entity 'palmitoylation' named entity 'plasmid' named entity 'cell fusion' named entity 'Lentiviral particles' named entity 'serum' named entity 'ezrin' named entity 'Carlsbad, CA' named entity 'M.O.' named entity 'Bitplane' named entity 'HIV' named entity 'plasma membrane' named entity 'mutation' named entity 'GFP' named entity 'amino acids' named entity 'SARS-CoV' named entity 'amino-acids' named entity 'qRT-PCR' named entity 'human embryonic' named entity 'pseudotyped' named entity 'ezrin'

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