About: Ocular pathogenesis and immune reaction after intravitreal dispase injection in mice

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Ocular pathogenesis and immune reaction after intravitreal dispase injection in mice
Creator	Li, Wei Tan, Juan Gao, Qianying Liu, Yaqin
Source	PMC
abstract	PURPOSE: The purpose of the current study was to examine the ocular pathogenesis and immune reaction in mice after intravitreal dispase injection. METHODS: Three microliters of dispase at a concentration of 0.2 U/μl were injected into the vitreal cavities of 4–6-week-old mice. Hematoxylin and eosin staining, immunofluorescence analysis, and electroretinograms of the eyes were then performed to assess ocular changes, and enzyme-linked immunospot assays and intracellular staining of single-cell suspensions of the spleens were used to detect immune changes during an 8 week observation period. RESULTS: Neutrophils were the main inflammatory infiltrating cells appearing at the anterior chamber. No cluster of differentiation (CD)3+ labeled T cells, F4/80+ labeled macrophages, or CD56+ labeled natural killer cells were found in the vitreal cavities or retinas in dispase-injected mice within 5 days after injection. Proliferative vitreoretinopathy (PVR)-like signs first appeared at 2 weeks, gradually increased thereafter, and reached peak values at 8 weeks. There was a statistically significant difference in b-wave amplitudes between the PVR and saline-control eyes. Enzyme-linked immunospot assays and intracellular staining showed that specific CD4+ and CD8+ labeled T cells were not involved in dispase-injected mice. CONCLUSIONS: Our data show that neutrophils in the anterior chamber and PVR-like signs in the retinas were found, and that specific immune reactions were not involved after intravitreal dispase injection in mice.
has issue date	2012-04-07(xsd:dateTime)
bibo:pmid	22511850
has license	cc-by
sha1sum (hex)	18a116d870bd24c96d95317418946ce0873f2180
resource representing a document's title	Ocular pathogenesis and immune reaction after intravitreal dispase injection in mice
has PubMed Central identifier	PMC3327440
has PubMed identifier	22511850
schema:publication	Mol Vis
resource representing a document's body	covid:18a116d870bd24c96d95317418946ce0873f2180#body_text
is schema:about of	named entity 'REACTION' named entity 'INJECTION' named entity 'IMMUNE' covid:arg/18a116d870bd24c96d95317418946ce0873f2180 named entity 'CD4' named entity 'PVR' named entity 'TO DETECT' named entity 'STATISTICALLY SIGNIFICANT' named entity 'OBSERVATION' named entity 'PATHOGENESIS' named entity 'PERFORMED' named entity '293' named entity 'PERIOD' named entity 'SIGNS' named entity 'INJECTION' named entity 'SINGLE' named entity 'HEMATOXYLIN AND EOSIN STAINING' named entity 'NOT INVOLVED' named entity 'PROLIFERATIVE VITREORETINOPATHY' named entity 'REACTION' named entity 'INFLAMMATORY' named entity 'INTRAVITREAL' named entity 'ANALYSIS' named entity 'OCULAR' named entity 'DATA' named entity 'CHANGES' named entity '8 WEEKS' named entity 'LABELED' named entity 'DISPASE' named entity 'MICE' named entity 'DISPASE' named entity 'MACROPHAGES' named entity 'IMMUNOFLUORESCENCE' named entity 'CLUSTER OF DIFFERENTIATION' named entity 'ENZYME-LINKED IMMUNOSPOT ASSAYS' named entity 'WAVE' named entity 'LIKE' named entity 'BCL' named entity 'CD8' named entity 'CELL' named entity 'T CELLS' named entity 'MICE' named entity 'METHODS' named entity 'CURRENT' named entity 'STUDY' named entity 'INFILTRATING CELLS' named entity 'OCULAR' named entity 'ASSESS' named entity 'RESULTS' named entity 'WEEK' named entity 'SALINE' named entity 'SPLEENS' named entity 'FOUND' named entity 'PEAK VALUES' named entity 'OUR' named entity 'EYES' named entity 'SUSPENSIONS' named entity 'NEUTROPHILS' named entity 'PURPOSE' named entity 'WAS A' named entity '0.2' named entity 'RETINAS' named entity 'USED' named entity 'INTRACELLULAR STAINING' named entity 'PATHOGENESIS' named entity 'ANTERIOR CHAMBER' named entity 'TO EXAMINE' named entity 'CONCLUSIONS' named entity 'CONTROL' named entity 'SPECIFIC' named entity '2 WEEKS' named entity 'NATURAL KILLER CELLS' named entity 'MAIN' named entity 'CONCENTRATION OF' named entity 'DIFFERENCE' named entity 'CD56' named entity 'INTRAVITREAL' named entity 'IMMUNE' named entity 'CAVITIES' named entity 'DAYS'

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