About: Reverse transcription recombinase-aided amplification assay combined with a lateral flow dipstick for detection of avian infectious bronchitis virus

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About: Reverse transcription recombinase-aided amplification assay combined with a lateral flow dipstick for detection of avian infectious bronchitis virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Reverse transcription recombinase-aided amplification assay combined with a lateral flow dipstick for detection of avian infectious bronchitis virus
Creator	Zhang, Peng Wang, Wenjing Lanzhou, † Liu, Jingru Wang, Chunguang Yao, Shanshan Zhai, Xianghe Zhang, Tie
Source	Medline; PMC
abstract	The study was conducted to develop a specific, simple, and sensitive method for diagnosis of avian infectious bronchitis virus (IBV). In this experiment, the selected downstream primer was labeled with biotin and the 5' end of RAA probe was labeled with FAM by reverse transcription recombinase-aided amplification (RT-RAA) combined with lateral flow dipstick (LFD). A RT-RAA-LFD assay that could be used for detection of IBV was established after optimization of RT-RAA reaction time, reaction temperature, and primer concentration. This method did not need reverse transcription of IBV template under isothermal condition (37°C), the amplification of target gene fragments could be completed within only 24 min, and the amplification products could be visually observed and determined by LFD within 3 min. The specificity test demonstrated that there was no cross reaction with the nucleic acids of other similar common pathogens. The lowest detectable limit for IBV was 10(2) copies/μL, and this method was 100 times more sensitive than conventional PCR (10(4) copies/μL), as verified by sensitivity test. The results showed that RT-RAA-LFD assay with strong specificity and high sensitivity was simple and easy to operate, and could be used for rapid detection of IBV in clinical diagnosis.
has issue date	2019-09-20(xsd:dateTime)
bibo:doi	10.3382/ps/pez559
bibo:pmid	31539067
has license	no-cc
sha1sum (hex)	18dc497dbade61ee314c46981c7d7260b21869b7
schema:url	https://doi.org/10.3382/ps/pez559
resource representing a document's title	Reverse transcription recombinase-aided amplification assay combined with a lateral flow dipstick for detection of avian infectious bronchitis virus
has PubMed Central identifier	PMC7199787
has PubMed identifier	31539067
schema:publication	Poult Sci
resource representing a document's body	covid:18dc497dbade61ee314c46981c7d7260b21869b7#body_text
is schema:about of	named entity 'determined' named entity 'simple' named entity 'sensitive' named entity 'PCR' named entity 'DETECTION' named entity 'NUCLEIC ACIDS' named entity 'OPERATE' named entity 'DOWNSTREAM' named entity 'METHOD' named entity 'RESULTS' named entity 'SIMILAR' named entity 'test' named entity 'experiment' named entity 'labeled' named entity 'selected' named entity 'condition' named entity 'dipstick' named entity 'detection' named entity 'specificity' named entity 'PCR' named entity 'conducted' named entity 'visually' named entity 'operate' named entity 'gene' named entity 'flow' named entity 'IBV' named entity 'nucleic acids' named entity 'dipstick' named entity 'avian infectious bronchitis virus' named entity 'reverse transcription' named entity 'primer' named entity 'lateral flow' named entity 'recombinase' named entity 'Reverse transcription' named entity 'IBV' named entity 'broilers' named entity 'primers' named entity 'concentration gradient' named entity 'IBV' named entity 'Reverse transcription' named entity 'veterinarians' named entity '6-carboxyfluorescein' named entity 'virus' named entity 'negative control' named entity 'genomes' named entity '5' end' named entity 'genomic DNA' named entity 'plasmid' named entity 'IBV' named entity 'IBV' named entity 'electrophoresis' named entity 'RNA' named entity 'purified water' named entity 'primers' named entity 'Infectious bronchitis virus' named entity 'PCR' named entity 'IBV' named entity '4-F' named entity 'primers' named entity '4-F' named entity 'point mutation' named entity 'IBV' named entity 'viruses' named entity 'China' named entity 'fluorophore' named entity 'IBV' named entity 'specificity and sensitivity' named entity 'control line' named entity 'agarose' named entity 'DNA replication' named entity 'nucleic acid' named entity 'plasmid' named entity '5' end' named entity 'primers'

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