About: Antibodies derived from a toxoid MEFA (multiepitope fusion antigen) show neutralizing activities against heat-labile toxin (LT), heat-stable toxins (STa, STb), and Shiga toxin 2e (Stx2e) of porcine enterotoxigenic Escherichia coli (ETEC)

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About: Antibodies derived from a toxoid MEFA (multiepitope fusion antigen) show neutralizing activities against heat-labile toxin (LT), heat-stable toxins (STa, STb), and Shiga toxin 2e (Stx2e) of porcine enterotoxigenic Escherichia coli (ETEC) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Antibodies derived from a toxoid MEFA (multiepitope fusion antigen) show neutralizing activities against heat-labile toxin (LT), heat-stable toxins (STa, STb), and Shiga toxin 2e (Stx2e) of porcine enterotoxigenic Escherichia coli (ETEC)
Creator	Casey, Thomas Duan, Qiangde Hashish, Emad Nandre, Rahul Rausch, Dana Ruan, Xiaosai Zhang, Weiping
Source	Elsevier; Medline; PMC
abstract	Enterotoxigenic Escherichia coli (ETEC) strains are the main cause of diarrhea in pigs. Pig diarrhea especially post-weaning diarrhea remains one of the most important swine diseases. ETEC bacterial fimbriae including K88, F18, 987P, K99 and F41 promote bacterial attachment to intestinal epithelial cells and facilitate ETEC colonization in pig small intestine. ETEC enterotoxins including heat-labile toxin (LT) and heat-stable toxins type Ia (porcine-type STa) and type II (STb) stimulate fluid hyper-secretion, leading to watery diarrhea. Blocking bacteria colonization and/or neutralizing enterotoxicity of ETEC toxins are considered effective prevention against ETEC diarrhea. In this study, we applied the MEFA (multiepitope fusion antigen) strategy to create toxoid MEFAs that carried antigenic elements of ETEC toxins, and examined for broad antitoxin immunogenicity in a murine model. By embedding STa toxoid STa(P12F) (NTFYCCELCCNFACAGCY), a STb epitope (KKDLCEHY), and an epitope of Stx2e A subunit (QSYVSSLN) into the A1 peptide of a monomeric LT toxoid (LT(R192G)), two toxoid MEFAs, ‘LT(R192G)-STb-Stx2e-STa(P12F)’ and ‘LT(R192G)-STb-Stx2e-3xSTa(P12F)’ which carried three copies of STa(P12F), were constructed. Mice intraperitoneally immunized with each toxoid MEFA developed IgG antibodies to all four toxins. Induced antibodies showed in vitro neutralizing activities against LT, STa, STb and Stx2e toxins. Moreover, suckling piglets born by a gilt immunized with ‘LT(R192G)-STb-Stx2e-3xSTa(P12F)’ were protected when challenged with ETEC strains, whereas piglets born by a control gilt developed diarrhea. Results from this study showed that the toxoid MEFA induced broadly antitoxin antibodies, and suggested potential application of the toxoid MEFA for developing a broad-spectrum vaccine against ETEC diarrhea in pigs.
has issue date	2016-02-06(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2016.02.002
bibo:pmid	26878972
has license	no-cc
sha1sum (hex)	191350faa97b3279eb49a1b4135ed0a6a6d8985b
schema:url	https://doi.org/10.1016/j.vetmic.2016.02.002
resource representing a document's title	Antibodies derived from a toxoid MEFA (multiepitope fusion antigen) show neutralizing activities against heat-labile toxin (LT), heat-stable toxins (STa, STb), and Shiga toxin 2e (Stx2e) of porcine enterotoxigenic Escherichia coli (ETEC)
has PubMed Central identifier	PMC7172483
has PubMed identifier	26878972
schema:publication	Vet Microbiol
resource representing a document's body	covid:191350faa97b3279eb49a1b4135ed0a6a6d8985b#body_text
is schema:about of	named entity 'colonization' named entity 'antigenic' named entity 'toxins' named entity 'small intestine' named entity 'study' named entity 'swine' named entity 'broadly' named entity 'pigs' named entity 'fusion' named entity 'FUSION' named entity 'CREATE' named entity 'STABLE' named entity 'TOXINS' named entity 'CONSTRUCTED' named entity 'MAIN' named entity 'ELEMENTS' named entity 'SPECTRUM' named entity 'CAUSE' named entity 'GILT' named entity 'BORN' named entity 'IMMUNIZED' named entity 'TOXIN' named entity 'VACCINE' named entity 'F18' named entity 'EXAMINED FOR' named entity 'gilt' named entity 'IgG' named entity 'diarrhea' named entity 'born' named entity 'attachment' named entity 'bacterial' named entity 'hyper-secretion' named entity 'carried' named entity 'murine' named entity 'stimulate' named entity 'STa' named entity 'neutralizing' named entity 'piglets' named entity 'leading' named entity 'ETEC' named entity 'IgG antibodies' named entity 'toxin' named entity 'epitope' named entity 'Enterotoxigenic Escherichia coli' named entity 'toxoid' named entity 'type II' named entity 'ETEC' named entity 'diarrhea' named entity 'small intestine' named entity 'antigen' named entity 'Shiga toxin' named entity 'toxin' named entity 'suckling' named entity 'colonization' named entity 'adjuvant' named entity 'cAMP' named entity 'lacZ' named entity 'Pig' named entity 'chimeric gene' named entity 'titration' named entity 'diarrhea' named entity 'swine' named entity 'IgG' named entity 'antitoxin' named entity 'antigen' named entity 'F-12' named entity 'BamH1' named entity 'recombinant strains' named entity 'Vero' named entity 'epitopes' named entity 'peptide' named entity 'Vero' named entity 'antibody' named entity 'maltose binding protein' named entity 'Pig'

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