About: Untargeted liquid chromatography coupled with mass spectrometry reveals metabolic changes in nitrogen-deficient Isatis indigotica Fortune

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About: Untargeted liquid chromatography coupled with mass spectrometry reveals metabolic changes in nitrogen-deficient Isatis indigotica Fortune Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Untargeted liquid chromatography coupled with mass spectrometry reveals metabolic changes in nitrogen-deficient Isatis indigotica Fortune
Creator	Wang, Lei Zhou, Ying Cao, Yi-Wen Geng, Li Miao, Yu-Jing Qu, Ren-Jun Tang, Xiao-Qing
Source	Elsevier; Medline; PMC
abstract	Abstract Isatis indigotica Fortune is a popular herb in traditional Chinese medicine, and various types of metabolites are the basis for its pharmacological efficacy. The biosynthesis and accumulation of these metabolites are closely linked to nitrogen availability; the benefits of low nitrogen application on the environment and herb quality are increasingly prominent. To analyze metabolic changes in the leaves and roots of I.indigotica in nitrogen deficiency conditions, and to identify the pathways and metabolites induced by low nitrogen availability, we used untargeted liquid chromatography coupled with mass spectrometry (UHPLC-TripleTOF) to obtain metabolomics profiling of I.indigotica under two N-deficiency treatments (0 kg/hm2; 337.5 kg/hm2) and normal nitrogen treatment (675 kg/hm2). A total of 447 metabolites were annotated. Principal component analysis separated the three nitrogen treatments. A greater diversity of metabolites was observed in roots than in leaves under N-deficiency treatments, suggesting that roots have a more important function in low N tolerance. Differential metabolites were mainly enriched in purine metabolism, phenylpropanoid biosynthesis, the shikimate pathway, tryptophan metabolism, and flavonoid biosynthesis that notably induced only in leaves in low nitrogen stress. Moderate N-deficiency benefits carbohydrate accumulation, whereas accumulation of most amino acids decreases. Uniquely, L-tryptophan was maintained at a high concentration in N-deficiency conditions. Low nitrogen stress induced the accumulation of some specialized metabolites (matairesinol, dictamnine, 5-hydroxyindoleacetate (serotonin) in roots and vitexin, xanthohumol, sinapyl alcohol in leaves). N-deficiency also increased the accumulation of adenosine and quality indicators of I.indigotica (indirubin-indigo, epigoitrin and anthranilic acid) in a certain degree. Our findings showed that nitrogen deficiency modified roots and leaves conditions of I.indigotica, affecting both the primary and secondary metabolism. Moderate nitrogen reduction was beneficial to the accumulation of active ingredients. Our methods and analysis are expected to provide an insight regarding the diversity of metabolites and regulation of their synthesis in low nitrogen application, and better investigate the nitrogen deficiency effect on I.indigotica.
has issue date	2019-10-31(xsd:dateTime)
bibo:doi	10.1016/j.phytochem.2019.112058
bibo:pmid	31280093
has license	els-covid
sha1sum (hex)	193a2cf373372b4c265e06cfddb3f874840d24c9
schema:url	https://doi.org/10.1016/j.phytochem.2019.112058
resource representing a document's title	Untargeted liquid chromatography coupled with mass spectrometry reveals metabolic changes in nitrogen-deficient Isatis indigotica Fortune
has PubMed Central identifier	PMC7111722
has PubMed identifier	31280093
schema:publication	Phytochemistry
resource representing a document's body	covid:193a2cf373372b4c265e06cfddb3f874840d24c9#body_text
is schema:about of	named entity 'metabolites' named entity 'accumulation' named entity 'shikimate pathway' named entity 'application' named entity 'nitrogen' named entity 'high' named entity 'nitrogen' named entity 'metabolites' named entity 'maintained' named entity 'Moderate' named entity 'reveals' named entity 'REVEALS' named entity 'ISATIS INDIGOTICA' named entity 'DEFICIENT' named entity 'LIQUID CHROMATOGRAPHY ' named entity 'ISATIS INDIGOTICA' named entity 'MAINTAINED' named entity 'ADENOSINE' named entity 'DEFICIENCY' named entity 'PHENYLPROPANOID BIOSYNTHESIS' named entity 'ENVIRONMENT' named entity 'INSIGHT' named entity 'THESE' named entity 'AFFECTING' named entity 'NORMAL' named entity 'NITROGEN' named entity 'TO IDENTIFY' named entity 'SINAPYL ALCOHOL' named entity 'CONDITIONS' named entity 'QUALITY INDICATORS' named entity 'INDUCED' named entity 'FORTUNE' named entity 'DECREASES' named entity 'ACCUMULATION' named entity 'PROMINENT' named entity 'FLAVONOID BIOSYNTHESIS' named entity 'THEIR' named entity 'METHODS' named entity 'L-TRYPTOPHAN' named entity 'HIGH CONCENTRATION' named entity 'FUNCTION' named entity 'BENEFICIAL' named entity 'STRESS' named entity 'NITROGEN' named entity 'IMPORTANT' named entity 'METABOLOMICS' named entity 'TREATMENTS' named entity 'FORTUNE' named entity 'METABOLIC' named entity 'COUPLED' named entity 'EXPECTED' named entity 'CHANGES' named entity 'XANTHOHUMOL' named entity 'BASIS' named entity 'INCREASED' named entity 'PATHWAYS' named entity 'TRADITIONAL CHINESE MEDICINE' named entity 'QUALITY' named entity '5-HYDROXYINDOLEACETATE' named entity 'PRIMARY' named entity 'MASS SPECTROMETRY' named entity 'METABOLITES' named entity 'BENEFITS' named entity 'HERB' named entity 'EFFICACY' named entity 'CHANGES' named entity 'REDUCTION' named entity 'DEGREE' named entity 'ENRICHED' named entity 'SECONDARY METABOLISM' named entity 'SPECIALIZED' named entity 'AMINO ACIDS' named entity 'USED' named entity 'METABOLIC' named entity 'EFFECT'

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