About: Rapid, field-deployable nucleobase detection and identification using FnCas9

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid, field-deployable nucleobase detection and identification using FnCas9
Creator	Kumar, Manoj Makharia, Govind Singhal, Khushboo Sharma, Namrata Aich, Meghali Azhar, Mohd Chakraborty, Debojyoti Chandak, Giriraj Hussain Ansari, Asgar Lad, Harsha Maiti, Souvik Patra, Pradeep Phutela, Rhythm Rauthan, Riya Sharma, Saumya Sinha, Dipanjali
Source	BioRxiv
abstract	Detection of pathogenic sequences or variants in DNA and RNA through a point-of-care diagnostic approach is valuable for rapid clinical prognosis. In recent times, CRISPR based detection of nucleic acids has provided an economical and quicker alternative to sequencing-based platforms which are often difficult to implement in the field. Here, we present FnCas9 Editor Linked Uniform Detection Assay (FELUDA) that employs a highly accurate enzymatic readout for detecting nucleotide sequences, identifying nucleobase identity and inferring zygosity with precision. We demonstrate that FELUDA output can be adapted to multiple signal detection platforms and can be quickly designed and deployed for versatile applications including rapid diagnosis during infectious disease outbreaks like COVID-19.
has issue date	2020-04-21(xsd:dateTime)
bibo:doi	10.1101/2020.04.07.028167
has license	biorxiv
sha1sum (hex)	19eecfa01781076586704613e7d2c8f9ec5a6a3b
schema:url	https://doi.org/10.1101/2020.04.07.028167
resource representing a document's title	Rapid, field-deployable nucleobase detection and identification using FnCas9
schema:publication	bioRxiv
resource representing a document's body	covid:19eecfa01781076586704613e7d2c8f9ec5a6a3b#body_text
is schema:about of	named entity 'detecting' named entity 'nucleobase' named entity 'TIMES' named entity 'ADAPTED' named entity 'APPLICATIONS' named entity 'READOUT' named entity 'POINT-OF-CARE' named entity 'ZYGOSITY' named entity 'FELUDA' named entity 'nucleic acids' named entity 'Detection' named entity 'identity' named entity 'CRISPR' named entity 'point-of-care diagnostic' named entity 'FELUDA' named entity 'accurate' named entity 'identifying' named entity 'primer' named entity 'RNP' named entity 'FELUDA' named entity 'denatured' named entity 'binding affinity' named entity 'TracrRNA' named entity 'DNA sequences' named entity 'signal amplification' named entity '4 nM' named entity 'Hemophilia' named entity 'Assay' named entity 'Sanger sequencing' named entity 'SCA' named entity 'mutation' named entity 'antibiotic resistance' named entity 'Rajesh Pandey' named entity 'Thermo Fisher Scientific' named entity 'Thermo fisher' named entity 'saliva' named entity 'SNVs' named entity 'signal amplification' named entity 'infectious diseases' named entity 'gene' named entity 'pathogenic microorganisms' named entity '8.5' named entity 'sgRNA' named entity 'sgRNA' named entity 'Urea' named entity 'pH 8' named entity 'CRISPR' named entity 'plasmid' named entity 'DNase' named entity 'SNV' named entity 'primer' named entity 'point mutation' named entity 'genotype' named entity 'FELUDA' named entity 'sgRNA' named entity 'biopsy' named entity 'FELUDA' named entity 'NaOAc' named entity 'SCA' named entity 'substrates' named entity 'RNP' named entity 'glycerol' named entity 'SCA' named entity 'FELUDA' named entity 'signal amplification' named entity 'RNP' named entity 'wild type' named entity 'synthetic DNA' named entity 'rRNA'

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