About: Comparison of Automated SARS-CoV-2 Antigen Test for COVID-19 Infection with Quantitative RT-PCR using 313 Nasopharyngeal Swabs Including from 7 Serially Followed Patients

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Comparison of Automated SARS-CoV-2 Antigen Test for COVID-19 Infection with Quantitative RT-PCR using 313 Nasopharyngeal Swabs Including from 7 Serially Followed Patients Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparison of Automated SARS-CoV-2 Antigen Test for COVID-19 Infection with Quantitative RT-PCR using 313 Nasopharyngeal Swabs Including from 7 Serially Followed Patients
Creator	Omata, Masao Hirotsu, Yosuke Mochizuki, Hitoshi Amemiya, Kenji Hosaka, Kazuhiro Kakizaki, Yumiko Maejima, Makoto Miyashita, Yoshihiro Nagakubo, Yuki Shibusawa, Masahiro Sueki, Hitomi Tsutsui, Toshiharu Hayakawa, Miyoko Kojima, Satoshi Yagi, Shintaro
Source	Elsevier; Medline; PMC
abstract	Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is determined by reverse-transcription PCR (RT-PCR) in routine clinical practice. In the current pandemic situation, a more rapid and high-throughput method is in growing demand. Here, we validated the performance of a new antigen test (LUMIPULSE) based on the chemiluminescence enzyme immunoassay. A total of 313 nasopharyngeal swabs (82 serial samples from 7 infected patients, 231 individual samples from 4 infected patients and 215 non-infected individuals) were analyzed for SARS-CoV-2 by quantitative RT-PCR (RT-qPCR) and then subjected to LUMIPULSE. We determined the cutoff value for antigen detection using receiver operating characteristic curve analysis and compared the antigen test performance with that of RT-qPCR. Further, we compared the viral loads and antigen levels in serial samples from seven infected patients. When using RT-qPCR as the reference, the antigen test exhibited 55.2% sensitivity and 99.6% specificity with a 91.4% overall agreement rate (286/313). In specimens with > 100 viral copies and between 10 and 100 copies, the antigen test showed 100% and 85% concordance with RT-qPCR, respectively. This concordance declined with lower viral loads. In the serially followed patients, the antigen levels showed a steady decline along with viral clearance. This gradual decline was in contrast with the abrupt “positive-to-negative” and “negative-to-positive” status changes observed with RT-qPCR, particularly in the late phase of infection. In summary, the LUMIPULSE antigen test can rapidly identify SARS-CoV-2-infected individuals with moderate to high viral loads and may be helpful for monitoring viral clearance in hospitalized patients.
has issue date	2020-08-12(xsd:dateTime)
bibo:doi	10.1016/j.ijid.2020.08.029
bibo:pmid	32800855
has license	els-covid
sha1sum (hex)	1a05120d06c7d02246043c250230c761cecc2c23
schema:url	https://doi.org/10.1016/j.ijid.2020.08.029
resource representing a document's title	Comparison of Automated SARS-CoV-2 Antigen Test for COVID-19 Infection with Quantitative RT-PCR using 313 Nasopharyngeal Swabs Including from 7 Serially Followed Patients
has PubMed Central identifier	PMC7422837
has PubMed identifier	32800855
schema:publication	Int J Infect Dis
resource representing a document's body	covid:1a05120d06c7d02246043c250230c761cecc2c23#body_text
is schema:about of	named entity 'viral' named entity 'high' named entity 'viral' named entity 'ASSESS' named entity 'ROUTINE' named entity 'DETERMINED BY' named entity 'SAMPLES' named entity 'ANTIGEN LEVEL' named entity 'DECLINING' named entity 'RT-QPCR' named entity 'Antigen' named entity 'RT-PCR' named entity 'antigen test' named entity 'PCR' named entity 'PCR' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'Infection' named entity 'samples' named entity 'Fujirebio' named entity 'infection' named entity 'antigen' named entity 'RNA' named entity 'SARS' named entity 'Epidemiologically' named entity 'RT-qPCR' named entity 'antigen' named entity 'COVID' named entity 'RT-qPCR' named entity 'Antigen' named entity 'PCR' named entity 'Fluorescent' named entity 'chemiluminescence' named entity 'SARS-CoV-2' named entity 'RT-qPCR' named entity 'RT-qPCR' named entity 'antigen test' named entity 'PCR' named entity 'antigen' named entity 'WHO' named entity 'viral titer' named entity 'RT-qPCR' named entity 'antigen test' named entity 'Statistical analysis' named entity 'Fujirebio' named entity 'RT-qPCR' named entity 'antigen test' named entity 'infection' named entity 'SARS-CoV-2' named entity '85%' named entity 'Receiver operating characteristic' named entity 'late phases' named entity 'healthcare workers' named entity 'antigen' named entity 'pg/mL' named entity 'antigen' named entity 'nasopharyngeal swabs' named entity 'quantitative RT-PCR' named entity 'Japan' named entity 'Leeds' named entity 'Japan' named entity 'SARS-CoV-2' named entity 'Japan' named entity 'antigen test' named entity 'infection' named entity 'Ph.D.' named entity 'SARS-CoV-2'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software