About: Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain

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About: Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain
Creator	Tian, Hong Ye, Jin Shang, Youjun Wu, Jinyan Feng Xia, Guozheng, Cong Haixue, Zheng Huichen, Guo Huiyun, Chang Jianhong, Guo Junwu, M Qingge, Xie Shiqi, Sun Shuanghui, Yin Xiangtao, Liu Xingwen, Bai Zaixin, Liu
Source	PMC
abstract	The full-length cDNA clone of swine vesicular disease virus HK/70 strain named pSVOK(12) was constructed in order to study the antigenicity, replication, maturation and pathogenicity of swine vesicular disease virus. In vitro transcription RNA from pSVOK(12) transfected IBRS-2 cells and the recovered virus RNA were isolated and sequenced, then indirect hemagglutination test, indirect immunofluorescence assays, eleectron microscope test, 50% tissue culture infecting dose (TCID(50)) assays and mouse virulence studies were performed to study the antigenicity and virulence of the recovered virus. The result showed that the infectious clones we obtained and the virus derived from pSVOK(12) had the same biological properties as the parental strain HK/70. The full-length infectious cDNA clone, pSVOK(12), will be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and replication of SVDV.
has issue date	2006-01-01(xsd:dateTime)
bibo:doi	10.1007/s11434-006-2095-z
bibo:pmid	32214723
has license	no-cc
sha1sum (hex)	1a31f9282d766f99d7d0b54918c072e15c1166ba
schema:url	https://doi.org/10.1007/s11434-006-2095-z
resource representing a document's title	Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain
has PubMed Central identifier	PMC7089143
has PubMed identifier	32214723
schema:publication	Chin Sci Bull
resource representing a document's body	covid:1a31f9282d766f99d7d0b54918c072e15c1166ba#body_text
is schema:about of	named entity 'tissue culture' named entity 'antigenicity' named entity 'maturation' named entity 'assays' named entity 'study' named entity 'indirect immunofluorescence' named entity 'replication' named entity 'virus' named entity 'study' named entity 'virus' named entity 'RNA' named entity 'indirect' named entity 'virus' named entity 'swine vesicular disease' named entity 'antigenicity' named entity 'virus' named entity 'virulence' named entity 'virus' named entity 'antigenicity' named entity 'swine vesicular disease' named entity 'microscope' named entity 'transfected' named entity 'virus' named entity 'viruses' named entity 'viruses' named entity 'RNA' named entity 'fluorescence microscope' named entity 'FMDV' named entity 'infectivity' named entity 'electrophoresed' named entity 'cDNA' named entity 'enzyme' named entity 'plasmid' named entity 'SVD' named entity 'cDNA clone' named entity 'RNA' named entity 'FMDV' named entity 'positive sense' named entity 'recombinant' named entity 'SVD' named entity 'cDNA' named entity 'attenuated vaccine' named entity 'pathogen' named entity 'OIE' named entity 'Invitrogen' named entity 'transfection' named entity 'Belgium' named entity 'polyprotein' named entity 'paralysis' named entity 'fluorescent' named entity 'EDTA' named entity 'amino acids' named entity 'nucleotides' named entity 'recombinant virus' named entity 'infectious agent' named entity 'cDNA' named entity 'viral protein' named entity 'transcription' named entity 'RNA' named entity 'virus' named entity '3′ UTR' named entity 'cell culture' named entity 'virus' named entity 'ascites fluid' named entity 'RT-PCR'

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