About: Molecular characterization of hepatitis E virus ORF1 gene supports a papain-like cysteine protease (PCP)-domain activity

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About: Molecular characterization of hepatitis E virus ORF1 gene supports a papain-like cysteine protease (PCP)-domain activity Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Molecular characterization of hepatitis E virus ORF1 gene supports a papain-like cysteine protease (PCP)-domain activity
Creator	Khalid Parvez, Mohammad
Source	Elsevier; Medline; PMC
abstract	Abstract Hepatitis E Virus (HEV) ORF1 encodes the nonstructural polyprotein wherein a role of PCP-domain in ORF1 proteolysis and/or RNA replication still remains contested. A series of ORF1 mutants of HEV-SAR55 replicon were constructed and tested for viability in S10-3 cells. Six of PCP-‘cysteine’ (C457A, C459A, C471A, C472A, C481A and C483A) and three ‘histidine’ (H443L, H497L and H590L) mutants were lethal. Further, a highly conserved ‘glycine-triad’ (G815-G816-G817) in downstream X-domain, homologous to rubella virus protease-substrate (G1299-G1300-G1301) was identified where two of X-mutants (G816V and G817V) turned lethal. However, all ORF1 sequential nucleotide-mutants conserving the amino acids were viable, which clearly showed post-translational regulation of HEV replication by PCP- and X-domains. Moreover, while vector-expressed ORF1-fusion polyprotein yielded a ∼191kDa band in vitro, it produced ∼78 and ∼35kDa fragments ex vivo. Collectively, the indispensability and functional effects of ‘PCP-catalytic’ and ‘X-substrate’ residues on HEV replication strongly supported a viral protease.
has issue date	2013-12-26(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2013.07.020
bibo:pmid	23978667
has license	els-covid
sha1sum (hex)	1b866f640fd6093ed5e3b7d2c5c0bc16bf042089
schema:url	https://doi.org/10.1016/j.virusres.2013.07.020
resource representing a document's title	Molecular characterization of hepatitis E virus ORF1 gene supports a papain-like cysteine protease (PCP)-domain activity
has PubMed Central identifier	PMC7125709
has PubMed identifier	23978667
schema:publication	Virus Research
resource representing a document's body	covid:1b866f640fd6093ed5e3b7d2c5c0bc16bf042089#body_text
is schema:about of	named entity 'PAPAIN-LIKE CYSTEINE PROTEASE' named entity 'ORF1' named entity 'turned' named entity 'viability' named entity 'conserving' named entity 'cysteine protease' named entity 'hepatitis E' named entity 'KDA' named entity 'POLYPROTEIN' named entity 'BAND' named entity 'REPLICON' named entity 'FUSION' named entity 'DOMAIN' named entity 'TRIAD' named entity 'EXPRESSED' named entity 'PROTEOLYSIS' named entity 'LETHAL' named entity 'CELLS' named entity 'HEV' named entity 'SUBSTRATE' named entity 'SERIES' named entity 'ACTIVITY' named entity 'TURNED' named entity 'HEPATITIS E VIRUS' named entity 'RNA REPLICATION' named entity 'MOLECULAR' named entity 'CHARACTERIZATION' named entity 'EFFECTS' named entity 'EX VIVO' named entity 'GENE' named entity 'SUPPORTED' named entity 'TESTED FOR' named entity 'HOMOLOGOUS' named entity 'ROLE' named entity 'ORF1' named entity 'SAR' named entity 'IN VITRO' named entity 'MUTANTS' named entity 'VIABLE' named entity 'IDENTIFIED' named entity 'PROTEASE' named entity 'AMINO ACIDS' named entity 'VIRAL' named entity 'HIGHLY' named entity 'POST-TRANSLATIONAL REGULATION' named entity 'ORF1' named entity 'FUNCTIONAL' named entity 'PCP' named entity 'SEQUENTIAL' named entity 'DOMAIN' named entity 'WHERE' named entity 'NUCLEOTIDE' named entity 'CONSERVED' named entity 'REPLICATION' named entity 'S10' named entity 'VECTOR' named entity 'CATALYTIC' named entity 'DOWNSTREAM' named entity 'VIABILITY' named entity 'FRAGMENTS' named entity 'CONSTRUCTED' named entity 'HEPATITIS E VIRUS' named entity 'RUBELLA VIRUS' covid:arg/1b866f640fd6093ed5e3b7d2c5c0bc16bf042089 named entity 'conserved' named entity 'mutants' named entity 'HEV' named entity 'band' named entity 'ORF1' named entity 'protease' named entity 'PCP' named entity 'histidine' named entity 'PCP' named entity 'glycine' named entity 'viral protease' named entity 'protease' named entity 'substrate' named entity 'substrate' named entity 'replicon' named entity 'HEV' named entity 'ORF1'

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