About: Role of Porcine Aminopeptidase N and Sialic Acids in Porcine Coronavirus Infections in Primary Porcine Enterocytes

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About: Role of Porcine Aminopeptidase N and Sialic Acids in Porcine Coronavirus Infections in Primary Porcine Enterocytes Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Role of Porcine Aminopeptidase N and Sialic Acids in Porcine Coronavirus Infections in Primary Porcine Enterocytes
Creator	Nauwynck, Hans Xie, Jiexiong Theuns, Sebastiaan Cui, Tingting Van Den Broeck, Wim
Source	Medline; PMC
abstract	Porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) have been reported to use aminopeptidase N (APN) as a cellular receptor. Recently, the role of APN as a receptor for PEDV has been questioned. In our study, the role of APN in PEDV and TGEV infections was studied in primary porcine enterocytes. After seven days of cultivation, 89% of enterocytes presented microvilli and showed a two- to five-fold higher susceptibility to PEDV and TGEV. A significant increase of PEDV and TGEV infection was correlated with a higher expression of APN, which was indicative that APN plays an important role in porcine coronavirus infections. However, PEDV and TGEV infected both APN positive and negative enterocytes. PEDV and TGEV Miller showed a higher infectivity in APN positive cells than in APN negative cells. In contrast, TGEV Purdue replicated better in APN negative cells. These results show that an additional receptor exists, different from APN for porcine coronaviruses. Subsequently, treatment of enterocytes with neuraminidase (NA) had no effect on infection efficiency of TGEV, implying that terminal cellular sialic acids (SAs) are no receptor determinants for TGEV. Treatment of TGEV with NA significantly enhanced the infection which shows that TGEV is masked by SAs.
has issue date	2020-04-05(xsd:dateTime)
bibo:doi	10.3390/v12040402
bibo:pmid	32260595
has license	cc-by
sha1sum (hex)	1b96d9b48734e5ac426941993ae13390ed2a76d9
schema:url	https://doi.org/10.3390/v12040402
resource representing a document's title	Role of Porcine Aminopeptidase N and Sialic Acids in Porcine Coronavirus Infections in Primary Porcine Enterocytes
has PubMed Central identifier	PMC7232180
has PubMed identifier	32260595
schema:publication	Viruses
resource representing a document's body	covid:1b96d9b48734e5ac426941993ae13390ed2a76d9#body_text
is schema:about of	named entity 'Miller' named entity 'APN' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'TERMINAL' named entity 'PRIMARY' named entity 'APN' named entity 'terminal' named entity 'efficiency' named entity 'study' named entity 'infected' named entity 'infection' named entity 'Porcine' named entity 'TGEV' named entity 'infection' named entity 'aminopeptidase' named entity 'Porcine epidemic diarrhea virus' named entity 'porcine' named entity 'PEDV' named entity 'PEDV' named entity 'porcine' named entity 'Enterocytes' named entity 'cellular receptor' named entity 'small intestinal' named entity 'centrifugation' named entity 'Wnt' named entity 'antibodies' named entity 'Wnt' named entity 'paraformaldehyde' named entity 'lectin' named entity 'PEDV' named entity 'Faculty of Veterinary Medicine' named entity 'type 1' named entity 'paraformaldehyde' named entity 'Maackia amurensis' named entity 'DMEM' named entity 'Roche Diagnostics' named entity 'epithelial cells' named entity 'SARS-CoV' named entity 'enterocytes' named entity 'enterocytes' named entity 'porcine' named entity 'infection' named entity 'immunofluorescence' named entity 'Streptavidin' named entity 'infection' named entity 'porcine' named entity 'infection' named entity 'PEDV' named entity 'enterocytes' named entity 'insulin' named entity 'ileum' named entity 'goat' named entity 'enterocytes' named entity 'TGEV' named entity 'fluorescence microscopy' named entity 'carbohydrates' named entity 'enterocytes' named entity 'insulin' named entity 'TGEV' named entity 'staining' named entity 'enterocytes' named entity 'small intestines' named entity 'epithelial' named entity 'hydrocortisone' named entity 'Prof.' named entity 'serine' named entity 'colon' named entity 'enterocytes' named entity 'centrifugation' named entity 'cell line' named entity 'intestinal tract' named entity 'DMEM' named entity 'PEDV' named entity 'lower concentration' named entity 'lumen' named entity 'spermidine' named entity 'co-culture'

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