About: Evaluation by polymerase chain reaction of cytomegalovirus reactivation in intensive care patients under mechanical ventilation

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About: Evaluation by polymerase chain reaction of cytomegalovirus reactivation in intensive care patients under mechanical ventilation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Evaluation by polymerase chain reaction of cytomegalovirus reactivation in intensive care patients under mechanical ventilation
Creator	Bernaudin, J.-E Beruaudin, Clergue, E Fajac, A Fajac, J.-E M6harzi, D Mtharzi, D Ricci, Ricci, S St6phan, F Sttphan, E
topic	covid:1bcbd34ae5628dd3b8f35f3ecae1279b7f6b361f#this
source	PMC
abstract	OBJECTIVE: The study was undertaken to determine if critically ill patients under mechanical ventilation could reactivate latent cytomegalovirus (CMV) in either lung or blood. DESIGN: Prospective study in critically ill patients. SETTING: The study was performed in a multidisciplinary intensive care unit in a university hospital. PATIENTS: 23 non-immunocompromised, mechanically ventilated patients who were anti-CMV immunoglobulin G-positive. Ten immunocompromised patients with active CMV infection and 16 asymptomatic CMV seropositive non-immunocompromised patients constituted the positive and negative control groups. MEASUREMENTS AND RESULTS: The presence of CMV in blood and bronchoalveolar lavage (BAL) was evaluated by both viral cultures and polymerase chain reaction (PCR). Thirty-seven blood and 22 BAL samples were investigated. Sequential samples were evaluated in 8 patients. For PCR, a 290 bp fragment in the first exon of the immediate early 1 gene was amplified. In order to exclude inhibitors of PCR amplification, a 268 bp fragment of the β-globin gene was concurrently amplified in all samples. Viral cultures of blood and BAL were negative in all 23 non-immunocompromised, mechanically ventilated patients. Moreover, no CMV DNA could be amplified in blood or BAL samples, whereas a β-globin amplification was observed in all samples. CONCLUSION: In a series of 23 critically ill patients under mechanical ventilation who were seropositive for CMV, no reactivation of CMV in blood or lung was demonstrated.
has issue date	1996-01-01(xsd:dateTime)
bibo:doi	10.1007/bf01709343
bibo:pmid	9120120
has license	no-cc
sha1sum (hex)	1bcbd34ae5628dd3b8f35f3ecae1279b7f6b361f
schema:url	https://doi.org/10.1007/bf01709343
resource representing a document's title	Evaluation by polymerase chain reaction of cytomegalovirus reactivation in intensive care patients under mechanical ventilation
has PubMed Central identifier	PMC7094969
has PubMed identifier	9120120
schema:publication	Intensive Care Med
resource representing a document's body	covid:1bcbd34ae5628dd3b8f35f3ecae1279b7f6b361f#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/1bcbd34ae5628dd3b8f35f3ecae1279b7f6b361f
is schema:about of	named entity 'Evaluation' named entity 'EITHER' named entity 'cytomegalovirus' named entity 'CMV' named entity 'CMV infection' named entity 'viral culture' named entity 'CMV' named entity 'immunoglobulin' named entity 'critically ill patients' named entity 'intensive care' named entity 'CMV' named entity 'sodium chloride' named entity 'perfusion' named entity 'critically ill' named entity 'CMV' named entity 'PCR' named entity 'White blood cells' named entity 'bronchoalveolar lavage' named entity 'neutrophils' named entity 'tumor necrosis factor' named entity 'diagnostic method' named entity 'control group' named entity 'ultraviolet' named entity 'genome' named entity 'endothelial cells' named entity 'CMV' named entity 'cardiac surgery' named entity 'septic shock' named entity 'myocardial infarction' named entity 'intensive care unit' named entity '2, 3' named entity 'CMV' named entity 'gene' named entity 'primer' named entity 'Proteinase' named entity 'immunocompetent' named entity 'CMV' named entity 'CMV' named entity 'reaction times' named entity 'CMV' named entity 'CMV' named entity 'immunocompromised patients' named entity 'DNA extraction' named entity 'globin' named entity 'denatured' named entity 'France' named entity 'polymerase chain reaction' named entity 'infection' named entity 'proteinase' named entity 'filtration' named entity 'pneumonia' named entity 'bronchoalveolar lavage' named entity 'denaturation' named entity 'EDTA' named entity 'Septic shock' named entity 'blood sample' named entity 'blood transfusion' named entity 'ammonium bicarbonate' named entity 'Cytocentrifuge' named entity 'CMV' named entity 'acquired immunity' named entity 'critically ill' named entity 'critically ill patients' named entity 'IgG' named entity 'CMV' named entity 'PCR technique' named entity 'CMV' named entity 'blood transfusion' named entity 'CMV'

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