About: Chloroquine Inhibits Dengue Virus Type 2 Replication in Vero Cells but Not in C6/36 Cells

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About: Chloroquine Inhibits Dengue Virus Type 2 Replication in Vero Cells but Not in C6/36 Cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Chloroquine Inhibits Dengue Virus Type 2 Replication in Vero Cells but Not in C6/36 Cells
Creator	Farias, Silva Juvenal, Kleber Lima Machado, Paula Lopes Da Fonseca, Benedito
source	Medline; PMC
abstract	Dengue viruses are the most important arthropod-borne viruses in terms of morbidity and mortality in the world. Since there is no dengue vaccine available for human use, we have set out to investigate the use of chloroquine as an antiviral drug against dengue. Chloroquine, an amine acidotropic drug known to affect intracellular exocytic pathways by increasing endosomal pH, was used in the in vitro treatment of Vero and C6/36 cells infected with dengue virus type 2 (DENV-2). Real-time RT-PCR and plaque assays were used to quantify the DENV-2 load in infected Vero and C6/36 cells after chloroquine treatment. Our results showed that a dose of 50 μg/ml of chloroquine was not toxic to the cells and induced a statistically significant inhibition of virus production in infected Vero cells when compared to untreated cells. In C6/36 cells, chloroquine does not induce a statistically significant difference in viral replication when compared to untreated cells, showing that this virus uses an unlikely pathway of penetration in these cells, and results were also confirmed by the plaque assay (PFU). These data suggest that the inhibition of virus infection induced by chloroquine is due to interference with acidic vesicles in mammalian cells.
has issue date	2013-01-31(xsd:dateTime)
bibo:doi	10.1155/2013/282734
bibo:pmid	23431254
has license	cc-by
sha1sum (hex)	1bebd833b3535712b5d13f9ed985e3491d3c96c8
schema:url	https://doi.org/10.1155/2013/282734
resource representing a document's title	Chloroquine Inhibits Dengue Virus Type 2 Replication in Vero Cells but Not in C6/36 Cells
has PubMed Central identifier	PMC3572654
has PubMed identifier	23431254
schema:publication	ScientificWorldJournal
resource representing a document's body	covid:1bebd833b3535712b5d13f9ed985e3491d3c96c8#body_text
is schema:about of	named entity 'REPLICATION' named entity 'induced' named entity 'type' named entity 'Our' named entity 'DUE TO' named entity 'USE OF' named entity 'IN VITRO' named entity 'DATA' named entity 'VESICLES' named entity 'REAL-TIME RT-PCR' named entity 'TO INVESTIGATE' named entity 'ACIDIC' named entity 'AFFECT' named entity 'OUR' named entity 'TREATMENT' named entity 'INCREASING' named entity 'PATHWAYS' named entity 'DOSE' named entity 'CHLOROQUINE' named entity 'INDUCED' named entity 'MAMMALIAN' named entity 'INTRACELLULAR' named entity 'IMPORTANT' named entity 'VERO' named entity 'DENGUE' named entity 'UNLIKELY' named entity 'HUMAN USE' named entity 'UNTREATED' named entity 'AN AMINE' named entity 'DENGUE VIRUS TYPE 2' named entity 'INHIBITION' named entity 'INFECTION INDUCED' named entity 'ANTIVIRAL DRUG' named entity 'WORLD' named entity 'AVAILABLE' named entity 'BUT' named entity 'VERO CELLS' named entity 'VACCINE' named entity 'HAVE' named entity 'STATISTICALLY SIGNIFICANT' named entity 'DENGUE VIRUS TYPE 2' named entity 'USES' named entity 'SUGGEST' named entity 'COMPARED' named entity 'DOES NOT' named entity 'THESE' named entity 'RESULTS' named entity 'VIRAL REPLICATION' named entity 'DRUG' named entity 'VIRUS PRODUCTION' named entity 'SET' named entity 'PLAQUE' named entity 'KNOWN' named entity 'TOXIC' named entity 'QUANTIFY' named entity 'DENGUE VIRUSES' named entity 'USED' named entity 'ARTHROPOD-BORNE VIRUSES' named entity 'INTERFERENCE' named entity 'INDUCE' named entity 'MORBIDITY AND MORTALITY' named entity 'VIRUS' named entity 'DIFFERENCE' named entity 'CONFIRMED BY' named entity 'CELLS' named entity 'CELLS' named entity 'INFECTED' named entity 'TERMS' named entity 'LOAD' named entity 'PATHWAY' named entity 'VIRUS INFECTION' named entity 'CHLOROQUINE' named entity 'VERO CELLS' named entity 'PENETRATION' named entity 'PLAQUE ASSAY' covid:arg/1bebd833b3535712b5d13f9ed985e3491d3c96c8 named entity 'morbidity' named entity 'virus'

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