About: Oligonucleotide Functionalised Microbeads: Indispensable Tools for High-Throughput Aptamer Selection

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About: Oligonucleotide Functionalised Microbeads: Indispensable Tools for High-Throughput Aptamer Selection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Oligonucleotide Functionalised Microbeads: Indispensable Tools for High-Throughput Aptamer Selection
Creator	Cheung, Yee-Wai Liang, Shaolin Lim, Bryce Jacques, Jean Miller, Alain Tanner, Julian Eynde, Vanden Dirkzwager, Roderick Fraser, Lewis Kinghorn, Andrew Tang, Marco
Source	PMC
abstract	The functionalisation of microbeads with oligonucleotides has become an indispensable technique for high-throughput aptamer selection in SELEX protocols. In addition to simplifying the separation of binding and non-binding aptamer candidates, microbeads have facilitated the integration of other technologies such as emulsion PCR (ePCR) and Fluorescence Activated Cell Sorting (FACS) to high-throughput selection techniques. Within these systems, monoclonal aptamer microbeads can be individually generated and assayed to assess aptamer candidate fitness thereby helping eliminate stochastic effects which are common to classical SELEX techniques. Such techniques have given rise to aptamers with 1000 times greater binding affinities when compared to traditional SELEX. Another emerging technique is Fluorescence Activated Droplet Sorting (FADS) whereby selection does not rely on binding capture allowing evolution of a greater diversity of aptamer properties such as fluorescence or enzymatic activity. Within this review we explore examples and applications of oligonucleotide functionalised microbeads in aptamer selection and reflect upon new opportunities arising for aptamer science.
has issue date	2015-12-01(xsd:dateTime)
bibo:doi	10.3390/molecules201219766
bibo:pmid	26633328
has license	cc-by
sha1sum (hex)	1c7230a768a9650c409a13c25b42dcf5f7f3392c
schema:url	https://doi.org/10.3390/molecules201219766
resource representing a document's title	Oligonucleotide Functionalised Microbeads: Indispensable Tools for High-Throughput Aptamer Selection
has PubMed Central identifier	PMC6332362
has PubMed identifier	26633328
schema:publication	Molecules
resource representing a document's body	covid:1c7230a768a9650c409a13c25b42dcf5f7f3392c#body_text
is schema:about of	named entity '1000' named entity 'CAPTURE' named entity 'HAVE' named entity 'COMMON' named entity 'FLUORESCENCE ACTIVATED DROPLET SORTING' named entity 'FLUORESCENCE' named entity 'MONOCLONAL' named entity 'ARISING' named entity 'GREATER' named entity 'ADDITION' named entity 'COMPARED' named entity 'EVOLUTION' named entity 'CANDIDATE' named entity 'FITNESS' named entity 'INTEGRATION' named entity 'APPLICATIONS' named entity 'SELECTION' covid:arg/1c7230a768a9650c409a13c25b42dcf5f7f3392c named entity 'TIMES' named entity 'INDISPENSABLE' named entity 'NON-' named entity 'BINDING' named entity 'CLASSICAL' named entity 'ENZYMATIC ACTIVITY' named entity 'THESE' named entity 'SELEX' named entity 'OLIGONUCLEOTIDE' named entity 'REFLECT' named entity 'EFFECTS' named entity 'EMULSION' named entity 'SEPARATION' named entity 'SYSTEMS' named entity 'DOES NOT' named entity 'PCR' named entity 'HIGH' named entity 'OLIGONUCLEOTIDE' named entity 'INDISPENSABLE' named entity 'APTAMER' named entity 'SELECTION' named entity 'THROUGHPUT' named entity 'MICROBEADS' named entity 'PROPERTIES' named entity 'PROTOCOLS' named entity 'RISE' named entity 'SCIENCE' named entity 'OLIGONUCLEOTIDES' named entity 'GENERATED' named entity 'TECHNIQUE' named entity 'TECHNIQUES' named entity 'HIGH' named entity 'TOOLS' named entity 'TRADITIONAL' named entity 'FLUORESCENCE ACTIVATED CELL SORTING' named entity 'APTAMER' named entity 'GIVEN' named entity 'REVIEW' named entity 'DIVERSITY' named entity 'MICROBEADS' named entity 'AFFINITIES' named entity 'ALLOWING' named entity 'ASSESS' named entity 'STOCHASTIC' named entity 'NEW' named entity 'THROUGHPUT' named entity 'TECHNOLOGIES' named entity 'Droplet' named entity 'aptamer' named entity 'aptamer' named entity 'SELEX' named entity 'high-throughput' named entity 'aptamer'

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