About: RNA-Dependent RNA Polymerase Speed and Fidelity are not the Only Determinants of the Mechanism or Efficiency of Recombination

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About: RNA-Dependent RNA Polymerase Speed and Fidelity are not the Only Determinants of the Mechanism or Efficiency of Recombination Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	RNA-Dependent RNA Polymerase Speed and Fidelity are not the Only Determinants of the Mechanism or Efficiency of Recombination
Creator	Zhao, Yan Kim, Hyejeong Woodman, Andrew Arnold, Jamie Cameron, Craig Ellis, Victor
Source	PMC
abstract	Using the RNA-dependent RNA polymerase (RdRp) from poliovirus (PV) as our model system, we have shown that Lys-359 in motif-D functions as a general acid in the mechanism of nucleotidyl transfer. A K359H (KH) RdRp derivative is slow and faithful relative to wild-type enzyme. In the context of the KH virus, RdRp-coding sequence evolves, selecting for the following substitutions: I331F (IF, motif-C) and P356S (PS, motif-D). We have evaluated IF-KH, PS-KH, and IF-PS-KH viruses and enzymes. The speed and fidelity of each double mutant are equivalent. Each exhibits a unique recombination phenotype, with IF-KH being competent for copy-choice recombination and PS-KH being competent for forced-copy-choice recombination. Although the IF-PS-KH RdRp exhibits biochemical properties within twofold of wild type, the virus is impaired substantially for recombination in cells. We conclude that there are biochemical properties of the RdRp in addition to speed and fidelity that determine the mechanism and efficiency of recombination. The interwoven nature of speed, fidelity, the undefined property suggested here, and recombination makes it impossible to attribute a single property of the RdRp to fitness. However, the derivatives described here may permit elucidation of the importance of recombination on the fitness of the viral population in a background of constant polymerase speed and fidelity.
has issue date	2019-11-25(xsd:dateTime)
bibo:doi	10.3390/genes10120968
bibo:pmid	31775299
has license	cc-by
sha1sum (hex)	1ce01bfd8909920c3da397b92d9803389f043268
schema:url	https://doi.org/10.3390/genes10120968
resource representing a document's title	RNA-Dependent RNA Polymerase Speed and Fidelity are not the Only Determinants of the Mechanism or Efficiency of Recombination
has PubMed Central identifier	PMC6947342
has PubMed identifier	31775299
schema:publication	Genes (Basel)
resource representing a document's body	covid:1ce01bfd8909920c3da397b92d9803389f043268#body_text
is schema:about of	named entity 'evaluated' named entity 'derivatives' named entity 'recombination' named entity 'BEING' named entity 'DERIVATIVE' named entity 'RECOMBINATION' named entity 'DESCRIBED' named entity 'DETERMINE' named entity 'POLIOVIRUS' named entity 'WILD-TYPE' named entity 'RELATIVE TO' named entity 'POLYMERASE' named entity 'WILD TYPE' named entity 'OF EACH' named entity 'CODING SEQUENCE' named entity 'GENERAL' named entity 'EFFICIENCY' named entity 'POPULATION' named entity 'SINGLE' named entity 'FIDELITY' named entity 'PROPERTIES' named entity 'SPEED' named entity 'COMPETENT' named entity 'UNDEFINED' named entity 'IMPAIRED' named entity 'TRANSFER' named entity 'CHOICE' named entity 'VIRUSES' named entity 'ENZYMES' named entity 'CONCLUDE' named entity 'FITNESS' named entity 'COPY' named entity 'DOUBLE MUTANT' named entity 'NATURE' named entity 'ENZYME' named entity 'IMPOSSIBLE' named entity 'RECOMBINATION' named entity 'BACKGROUND' named entity 'ACID' named entity 'CELLS' named entity 'RNA-DEPENDENT RNA POLYMERASE' named entity 'MECHANISM' named entity 'FOLLOWING' named entity 'EXHIBITS' covid:arg/1ce01bfd8909920c3da397b92d9803389f043268 named entity 'LYS-' named entity 'ATTRIBUTE' named entity 'UNIQUE' named entity 'DETERMINANTS' named entity 'USING' named entity 'RNA-DEPENDENT RNA POLYMERASE' named entity 'EFFICIENCY' named entity 'SPEED' named entity 'EQUIVALENT' named entity 'MODEL SYSTEM' named entity 'FUNCTIONS' named entity 'OUR' named entity 'EVALUATED' named entity 'ADDITION' named entity 'FORCED' named entity 'SLOW' named entity 'PHENOTYPE' named entity 'VIRAL' named entity 'FIDELITY' named entity 'HAVE' named entity 'PROPERTY' named entity 'VIRUS' named entity 'MOTIF' named entity 'SELECTING' named entity 'RDRP' named entity 'CONTEXT' named entity 'MECHANISM' named entity 'HERE' named entity 'PERMIT' named entity 'BIOCHEMICAL' named entity 'CONSTANT'

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