About: Constructing and transient expression of a gene cassette containing edible vaccine elements and shigellosis, anthrax and cholera recombinant antigens in tomato

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About: Constructing and transient expression of a gene cassette containing edible vaccine elements and shigellosis, anthrax and cholera recombinant antigens in tomato Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Constructing and transient expression of a gene cassette containing edible vaccine elements and shigellosis, anthrax and cholera recombinant antigens in tomato
Creator	Davod, Jafari Dehghan, · Fatemeh, Nayeri Honari, · Hosseini, Ramin
source	Medline; PMC
abstract	Shigella dysenteriae causing shigellosis is one of the diseases that threaten the health of human society in the developing countries. In Shigella, IpaD gene is one of the key pathogenic genes causing strong mucosal immune system reactions. Anthrax disease is caused by Bacillus anthracis. PA protective antigen is one of the subunits in anthrax toxin complex responsible for the transfer of other subunits into the cytosol of host cells. The 20 kDa subunit of PA (PA20) has the property of immunogenicity. CTxB or B subunit of Vibrio cholerae toxin (CT) is a non-toxic protein and has the function to transfer toxic subunit into cytosol of the host cells by binding to GM1 receptor. The aim of this study was to fuse PA20, ipaD and CTxB and transform tomato plants by this cassette in order to produce an oral vaccine against shigellosis, anthrax and cholera. CTxB was used for these two antigens as an immune adjuvant. IpaD and PA20 genes were cloned in pBI121 containing the CTxB gene and Extensin signal peptide. In order to evaluate the transient expression of Shigellosis, Anthrax and Cholera antigens, agro-infiltrated tomato tissues were inoculated with Agrobacterium tumefaciens containing the gene cassette. Cloning was confirmed by PCR, enzymatic digestion and sequencing techniques. Expression of the antigens was examined by SDS-PAGE, dot blot and ELISA. Maturate green fruits demonstrated the highest expression of the recombinant proteins. The first phase of this study was carried out for cloning and expressing of CtxB, ipaD and PA20 antigens in tomato. In the next phase, we aim to analyze the immunogenicity of this vaccine candidate in laboratory animals.
has issue date	2018-09-22(xsd:dateTime)
bibo:doi	10.1007/s11033-018-4385-3
bibo:pmid	30244396
has license	no-cc
sha1sum (hex)	1cff28b278c5d4574b33576dee4e8bbbafe83df8
schema:url	https://doi.org/10.1007/s11033-018-4385-3
resource representing a document's title	Constructing and transient expression of a gene cassette containing edible vaccine elements and shigellosis, anthrax and cholera recombinant antigens in tomato
has PubMed Central identifier	PMC7088786
has PubMed identifier	30244396
schema:publication	Mol Biol Rep
resource representing a document's body	covid:1cff28b278c5d4574b33576dee4e8bbbafe83df8#body_text
is schema:about of	named entity 'ANTIGENS' named entity 'sequencing' named entity 'study' named entity 'shigellosis' named entity 'cells' named entity 'toxic' named entity 'anthrax' named entity 'gene cassette' named entity 'ELEMENTS' named entity 'ANTHRAX' named entity 'GENE CASSETTE' named entity 'IMMUNE ADJUVANT' named entity 'AGROBACTERIUM TUMEFACIENS' named entity 'REACTIONS' named entity 'TRANSIENT EXPRESSION' named entity 'MUCOSAL IMMUNE SYSTEM' named entity 'NON-' named entity 'AGRO' named entity 'HUMAN' named entity 'CYTOSOL' named entity 'KDA' named entity 'INFILTRATED' named entity 'VIBRIO CHOLERAE TOXIN' named entity 'SIGNAL PEPTIDE' named entity 'SOCIETY' named entity 'ORDER' named entity 'TOXIC' named entity 'GM1' named entity 'IMMUNOGENICITY' named entity 'SEQUENCING TECHNIQUES' named entity 'TRANSFER' named entity 'CONTAINING' named entity 'GREEN ' covid:arg/1cff28b278c5d4574b33576dee4e8bbbafe83df8 named entity 'EXPRESSION' named entity 'CASSETTE' named entity 'HOST CELLS' named entity 'KEY' named entity 'RECOMBINANT PROTEINS' named entity 'IS A' named entity 'ORAL VACCINE' named entity 'ANTIGENS' named entity 'DEVELOPING COUNTRIES' named entity 'HEALTH' named entity 'FRUITS' named entity 'PROTEIN ' named entity 'CHOLERA' named entity 'ANTHRAX' named entity 'RECEPTOR' named entity 'SHIGELLOSIS' named entity 'FIRST PHASE' named entity 'ELISA' named entity 'CAUSED BY' named entity 'CONSTRUCTING' named entity 'GENE CASSETTE' named entity 'TOMATO' named entity 'THESE' named entity 'STRONG' named entity 'DOT BLOT' named entity 'FUNCTION' named entity 'CHOLERA' named entity 'CONTAINING' named entity 'A GENE' named entity 'EDIBLE VACCINE' named entity 'TRANSIENT EXPRESSION' named entity 'RECOMBINANT' named entity 'ENZYMATIC DIGESTION' named entity 'TO PRODUCE' named entity 'PATHOGENIC' named entity 'SHIGELLA DYSENTERIAE' named entity 'USED' named entity 'DISEASES' named entity 'COMPLEX' named entity 'CAUSING' named entity 'DEMONSTRATED' named entity 'VACCINE CANDIDATE' named entity 'TOXIC PROTEIN'

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