About: Aspects of the developmental morphology of california encephalitis virus in cultured vertebrate and arthropod cells and in mouse brain

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Aspects of the developmental morphology of california encephalitis virus in cultured vertebrate and arthropod cells and in mouse brain
Creator	Heyduk, Jaroslav Lyons, Michael
Source	Elsevier; Medline; PMC
abstract	Abstract An electron microscopic study was made of the replication of California encephalitis (La Crosse strain) in cultured vertebrate cells (Vero, African green monkey kidney), in a line of cultured mosquito cells (Aedes albopictus), and in brain tissue of suckling mice. Morphologically similar virus particles, approximately 95 nm in diameter, were encountered in all three systems, and a common mode of virus assembly and maturation appeared to obtain. Virus assembly was shown to occur exclusively at internal cytomembrane interfaces, the Golgi complex appearing as the initial assembly site, which site became less focal as infection progressed due to the proliferation of Golgi smooth membranes and the dilation of cisternae and vesicles. The assembly process involved viral budding into cisternal and vesicular lumina, the virion thereby acquiring its limiting membrane. The envelope of such intracellular virions exhibited a poorly defined fringe, approximately 8 nm in width, which appeared to undergo a maturational change as the virions were discharged from the cell, such that, extracellularly, virions displayed a well-developed fringe, approximately 12 nm wide—a change especially noteworthy in the case of virions in infected mouse brain. The presence was noted in infected Vero cells—and in one instance in an infected mosquito cell—of crescent-shaped segments of thickened cisternal membrane, which possibly represented an early phase of viral assembly in which nucleocapsid aligned itself in close apposition to a membrane segment preparatory to the initiation of budding. In areas of the cytoplasm adjacent to sites of viral assembly in neurons, a fine granulofibrillar matrix was frequently found, enmeshed in which were numbers of 50–60 nm spherical structures. In a low proportion of cells from infected mosquito cultures, dense granulofibrillar masses were found in the cytoplasm. In Vero cells, infection with CE virus was cytolytic, while in A. albopictus cells, no gross cytopathic effects were manifest, and persistently infected cultures developed upon subcultivation. However, less than 10% of challenged mosquito cells became productively infected and for a proportion of these, at least as determined by electron microscopy, the infection was lethal.
has issue date	1973-07-31(xsd:dateTime)
bibo:doi	10.1016/0042-6822(73)90112-8
bibo:pmid	4197414
has license	els-covid
sha1sum (hex)	1d12dd4d9f7317225b46d7be01e997332051c527
schema:url	https://doi.org/10.1016/0042-6822%2873%2990112-8
resource representing a document's title	Aspects of the developmental morphology of california encephalitis virus in cultured vertebrate and arthropod cells and in mouse brain
has PubMed Central identifier	PMC7111277
has PubMed identifier	4197414
schema:publication	Virology
resource representing a document's body	covid:1d12dd4d9f7317225b46d7be01e997332051c527#body_text
is schema:about of	named entity 'VIRUS' named entity 'CALIFORNIA' named entity 'CELLS' named entity 'CULTURED' named entity 'DEVELOPMENTAL' named entity 'MORPHOLOGY' named entity 'VERTEBRATE' named entity 'ARTHROPOD' named entity 'MOUSE BRAIN' named entity 'Vertebrate' named entity 'California' named entity 'plasma membrane' named entity 'salts' named entity 'sucrose' named entity 'virus particle' named entity 'serum' named entity 'mosquito' named entity 'virions' named entity 'virus' named entity 'virions' named entity 'developmental morphology' named entity 'nucleocapsids' named entity 'nucleus' named entity 'phosphate' named entity 'Assay' named entity 'yeast extract' named entity 'virus' named entity 'incubation' named entity 'Figure 8' named entity 'virus' named entity 'brain cells' named entity 'A. albopictus' named entity 'virus' named entity 'cytoplasmic' named entity 'microscopy' named entity 'intercellular spaces' named entity 'electron microscopic' named entity 'electron microscopy' named entity 'Virions' named entity 'mosquito' named entity 'Golgi' named entity 'virus' named entity 'extracellular' named entity 'infection' named entity 'viral assembly' named entity 'adsorbed' named entity 'viral budding' named entity 'virus' named entity 'microscope' named entity 'viruses' named entity 'infection' named entity 'pancreatin' named entity 'infection' named entity 'infection' named entity 'virus replication' named entity 'Aedes albopictus' named entity 'virus' named entity 'Cell cultures' named entity 'endoplasmic reticulum' named entity '12 nm' named entity 'infection' named entity 'virus replication' named entity 'vacuoles' named entity 'arthropods' named entity 'plasma membrane' named entity 'Virus' named entity 'Microtubules' named entity 'virions' named entity 'plasma membrane' named entity 'Extracellular' named entity 'inoculum' named entity 'septate junctions' named entity 'virions' named entity 'inoculation' named entity 'vesicles' named entity 'vacuoles' named entity 'paralysis' named entity 'virus' named entity 'intercellular spaces' named entity 'cytoplasmic matrix'

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