About: Infectious bronchitis virus from chickens in Al-Hasa, Saudi Arabia 2015-2016

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About: Infectious bronchitis virus from chickens in Al-Hasa, Saudi Arabia 2015-2016 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Infectious bronchitis virus from chickens in Al-Hasa, Saudi Arabia 2015-2016
Creator	Alhammadi, Mohamed Alsultan, Musaed Gomaa Hemida, Maged
source	Medline; PMC
abstract	AIM: This study aimed to isolate some of the currently circulating infectious bronchitis virus (IBV) strains from some broiler chicken farms in Al-Hasa and to do some molecular characteristics of these strains. MATERIALS AND METHODS: We collected 300 tissue specimens, including the trachea, bronchi, lungs, and kidneys from some four commercial chicken farms showing respiratory manifestations. We tested these tissue specimens by the real-time polymerase chain reaction (RT-PCR) and gel-based PCR. We selected some PCR positive samples for isolation in the embryonated chicken eggs (ECE). We sequenced some PCR-positive samples and conducted phylogenetic analysis based on the obtained sequences. RESULTS: Our molecular surveillance revealed that 31.6% of the tested specimens were IBV positive by PCR. We selected some positive specimens showing low Ct values by the qRT-PCR for virus isolation by the ECE. The infected eggs showed hemorrhage, dwarfing, and death in some cases after three passages in the ECE. We sequenced some of the positive PCR specimens and used the obtained sequences to draw the phylogenetic tree based on the partial IBV-ORF-1a, N, and S1 gene sequences. The phylogenetic trees based on the IBV-N and S1 gene sequences showed that the circulating IBV strains in Al-Hasa during 2016 was showing a high degree of identity to some strains from Taiwan and Italy. Meanwhile, the grouping of these strains based on the IBV-S1 sequences revealed that the currently circulating IBV strains in Al-Hasa belonged to Gr.I.7 along with strains from Taiwan. CONCLUSION: Our results confirmed the continuous circulation of the IBV among the chicken population in Al-Hasa despite the intensive application of vaccines against this virus.
has issue date	2019-03-19(xsd:dateTime)
bibo:doi	10.14202/vetworld.2019.424-433
bibo:pmid	31089313
has license	cc-by
sha1sum (hex)	1da61ea537e7b6a6b56a2ac371e9e95442078672
schema:url	https://doi.org/10.14202/vetworld.2019.424-433
resource representing a document's title	Infectious bronchitis virus from chickens in Al-Hasa, Saudi Arabia 2015-2016
has PubMed Central identifier	PMC6487242
has PubMed identifier	31089313
schema:publication	Vet World
resource representing a document's body	covid:1da61ea537e7b6a6b56a2ac371e9e95442078672#body_text
is schema:about of	named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'CIRCULATING' named entity 'STUDY' named entity 'HASA' named entity 'AIM' named entity 'CHARACTERISTICS' named entity 'FARMS' named entity 'ISOLATE' named entity 'BROILER CHICKEN' named entity 'MOLECULAR' named entity 'THESE' named entity 'STRAINS' named entity 'CURRENTLY' covid:arg/1da61ea537e7b6a6b56a2ac371e9e95442078672 named entity 'virus' named entity 'broiler chicken' named entity 'King Faisal' named entity 'kidney' named entity 'infectious bronchitis virus' named entity 'IBV' named entity 'trachea' named entity 'live attenuated' named entity 'denaturation' named entity 'Bio-Rad' named entity 'morbidity' named entity 'IBV' named entity 'Saudi Arabia' named entity 'Saudi Arabia' named entity 'infection' named entity 'Taiwan' named entity 'lungs' named entity 'IBV' named entity 'RNA polymerase' named entity 'Italy' named entity 'recombination' named entity 'GenBank' named entity 'kidneys' named entity 'Al-Hasa' named entity 'IBV' named entity 'Necropsy' named entity 'Illumina' named entity 'IBV' named entity 'IBV' named entity 'IBV' named entity 'Italy' named entity 'embryo' named entity 'vaccine' named entity 'kidneys' named entity 'IBV' named entity 'Al-Hasa' named entity 'rales' named entity 'infection' named entity 'exudate' named entity 'bronchitis' named entity 'RT-PCR' named entity 'chicken farms' named entity 'poultry farms' named entity 'kidneys' named entity 'infection' named entity 'Al-Hasa' named entity 'IBV' named entity 'RNA' named entity 'shed the virus' named entity 'Al-Hasa' named entity 'IBV' named entity 'kidneys' named entity 'IBV' named entity 'IBV' named entity 'Iran' named entity 'IBV' named entity 'King Faisal' named entity 'Middle East' named entity 'King Abdul-Aziz' named entity 'Al-Hasa' named entity 'trachea' named entity 'IBV' named entity 'IBV' named entity 'coding sequence' named entity 'IBV'

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