About: Characterization of turkey coronavirus from turkey poults with acute enteritis

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About: Characterization of turkey coronavirus from turkey poults with acute enteritis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Characterization of turkey coronavirus from turkey poults with acute enteritis
Creator	Hooper, Tom Schrader, Donna Lin, Tsang Wu, Ching Loa, Chien Thacker, H Bryan, Tom Tsai, Shih
topic	covid:1e90e5ac4f30837179cd3ff9f9f380cd4dcaeca8#this
Source	Elsevier; Medline; PMC
abstract	The present study was to characterize turkey coronavirus associated with turkey poult enteritis and mortality. Intestinal contents or intestines from affected turkey poults and inoculated turkey embryos contained coronaviruses as revealed by electron microscopy or were positive for turkey coronavirus by immunofluorescent antibody assay. Sucrose density gradient ultracentrifugation of the virus-containing intestinal homogenate yielded two opalescent bands corresponding to the buoyant densities of 1.14–1.15 and 1.18–1.20 g/ml, respectively. Coronaviral particles from intestinal contents or the sucrose density gradient preparation were mainly spherical in shape and had envelope and central depression. They were surrounded by a fringe of regularly spaced petal-shaped projections attached to the particles by a short stalk. Purified viruses hemagglutinated rabbit erythrocytes with a titer of 16. Major protein bands of purified viruses analyzed by SDS-PAGE were located at 200, 100–110, 50–60, and 30–35 kDa. The patterns of protein bands were consistent with those of Minnesota or Quebec turkey coronavirus isolates. A 568 bp nucleotide fragment of turkey coronavirus spike protein gene was amplified from RNA of inoculated turkey embryo intestine or purified virus. Sequence analysis of the 568 bp PCR product revealed high degree of identity with the corresponding spike protein gene sequence of human and bovine coronaviruses. The results indicated that turkey coronavirus was associated with turkey poults with acute enteritis.
has issue date	2002-01-03(xsd:dateTime)
bibo:doi	10.1016/s0378-1135(01)00447-3
bibo:pmid	11731170
has license	no-cc
sha1sum (hex)	1e90e5ac4f30837179cd3ff9f9f380cd4dcaeca8
schema:url	https://doi.org/10.1016/s0378-1135%2801%2900447-3
resource representing a document's title	Characterization of turkey coronavirus from turkey poults with acute enteritis
has PubMed Central identifier	PMC7117285
has PubMed identifier	11731170
schema:publication	Vet Microbiol
resource representing a document's body	covid:1e90e5ac4f30837179cd3ff9f9f380cd4dcaeca8#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/1e90e5ac4f30837179cd3ff9f9f380cd4dcaeca8
is schema:about of	named entity 'electron microscopy' named entity 'densities' named entity 'poult' named entity 'purified' named entity 'intestines' named entity 'ANTIBODY' named entity 'PARTICLES' named entity 'ACUTE ENTERITIS' named entity 'ASSAY' named entity 'NUCLEOTIDE' named entity 'REVEALED' named entity 'ELECTRON MICROSCOPY' named entity 'CORONAVIRUSES' named entity 'CONTAINED' named entity 'DENSITY GRADIENT ULTRACENTRIFUGATION' named entity 'TITER' named entity 'VIRUSES' named entity '1.14' named entity 'ASSOCIATED WITH' named entity '60%' named entity 'ACUTE ENTERITIS' named entity 'PRESENT' named entity 'INDICATED' covid:arg/1e90e5ac4f30837179cd3ff9f9f380cd4dcaeca8 named entity '200' named entity 'EMBRYOS' named entity 'PCR PRODUCT' named entity '100' named entity 'QUEBEC' named entity 'SHAPE' named entity 'PURIFIED' named entity 'TURKEY' named entity 'CHARACTERIZATION' named entity 'TURKEY CORONAVIRUS' named entity 'POULT' named entity '1.15' named entity 'HUMAN' named entity 'SURROUNDED BY' named entity 'ISOLATES' named entity 'HOMOGENATE' named entity 'OPALESCENT' named entity 'DEGREE' named entity 'REGULARLY' named entity 'IMMUNOFLUORESCENT' named entity 'INTESTINAL' named entity 'TURKEY' named entity 'PROJECTIONS' named entity 'DEPRESSION' named entity 'ENVELOPE' named entity 'RNA' named entity 'PREPARATION' named entity 'INTESTINE' named entity 'ENTERITIS' named entity 'MINNESOTA' named entity 'VIRUS' named entity 'RABBIT' named entity 'CENTRAL' named entity 'FRAGMENT OF' named entity 'KDA' named entity 'DENSITIES' named entity 'SDS-PAGE' named entity 'ATTACHED TO' named entity 'SPHERICAL' named entity 'AMPLIFIED' named entity 'TURKEY CORONAVIRUS' named entity 'AFFECTED' named entity 'BOVINE' named entity 'SHORT STALK' named entity 'ERYTHROCYTES' named entity 'SUCROSE' named entity 'TO CHARACTERIZE' named entity 'PROTEIN '

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