About: Regulation of aminopeptidase N (EC 3.4.11.2; APN; CD13) by interferon-γ on the HL-60 cell line

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About: Regulation of aminopeptidase N (EC 3.4.11.2; APN; CD13) by interferon-γ on the HL-60 cell line Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Regulation of aminopeptidase N (EC 3.4.11.2; APN; CD13) by interferon-γ on the HL-60 cell line
Creator	Ambriović-Ristov, Andreja Breljak, Davorka Gabrilovac, Jelka Upić, Barbara
Source	Elsevier; Medline; PMC
abstract	Abstract Membrane-bound peptidases play important roles in the regulation of local concentrations of various signalling peptides such as the growth factors, hormones, chemokines and cytokines. That is accomplished by means of their enzyme activity. Recently, membrane-bound peptidases have also been shown to act as receptors, receiving signals from as yet undefined ligands and transducing them into the cell interior. By using either or both of these mechanisms, peptidases interact with fundamental cellular functions: growth, differentiation, activation and death. This study addressed the effects of a T-cell derived cytokine, interferon-gamma (IFN-γ) on the activity of aminopeptidase N (APN), an ectoenzyme processing several signal peptides. Cells of a myelo-monocytic cell line HL-60 were used as a model system, and APN was assayed at the levels of mRNA, its membrane marker CD13, and the enzyme activity. Regulation of CD13/APN by IFN-γ was found at all three levels. The direction of regulation was time-dependent: an initial down-regulation seen 24 and 48 hrs after the onset of treatment with IFN-γ was replaced by an up-regulation after 72 and/or 96 hrs. Up-regulation of CD13/APN observed after 96 hrs was preceded by an up-regulation of APN mRNA reaching its maximum after 72 hrs. The IFN-γ-induced regulation of APN was due to membrane aminopeptidase N, since it could be completely abrogated by an APN blocking antibody WM-15. The delayed up-regulation of CD13/APN (observed after 72 and/or 96 hrs), required de novo protein synthesis as it could be abrogated by cycloheximide, an inhibitor of protein synthesis. Possible role of endogenous (IFN-γ-induced) TGF-β in mediating CD13/APN up-regulation could be excluded, since no TGF-β was found in supernatants of IFN-γ treated HL-60 cells. Thus, our data show regulation of CD13/APN on cells of myelo-monocytic origin by a T-cell derived cytokine, IFN-γ. A similar mechanism might play a role in inflammation.
has issue date	2005-04-22(xsd:dateTime)
bibo:doi	10.1016/j.lfs.2004.09.040
bibo:pmid	15792835
has license	els-covid
sha1sum (hex)	1ea5771f0aa7465576db06da2d161c0babd17cce
schema:url	https://doi.org/10.1016/j.lfs.2004.09.040
resource representing a document's title	Regulation of aminopeptidase N (EC 3.4.11.2; APN; CD13) by interferon-γ on the HL-60 cell line
has PubMed Central identifier	PMC7094246
has PubMed identifier	15792835
schema:publication	Life Sciences
resource representing a document's body	covid:1ea5771f0aa7465576db06da2d161c0babd17cce#body_text
is schema:about of	named entity 'aminopeptidase N' named entity 'regulation' named entity 'CD13' named entity 'The' named entity 'levels' named entity 'Regulation' named entity 'CD13' named entity 'APN' named entity 'IMPORTANT' named entity 'CYTOKINE' named entity 'MONOCYTIC' named entity 'INTERFERON-GAMMA' named entity 'DEPENDENT' named entity 'INITIAL' named entity 'POSSIBLE' named entity 'OUR' named entity 'INTERACT' named entity 'APN' named entity 'MARKER' named entity 'HL-60' named entity 'INHIBITOR' named entity 'MEMBRANE' named entity 'EITHER' named entity 'UNDEFINED' named entity 'SIGNAL PEPTIDES' named entity 'DERIVED' named entity 'A T' named entity 'EXCLUDED' named entity 'REPLACED BY' named entity 'DE NOVO' named entity 'CELL LINE' named entity 'THEIR' named entity 'REGULATION' named entity 'PLAY' named entity 'DIRECTION' named entity 'EFFECTS' named entity 'COULD BE' named entity 'PEPTIDASES' named entity 'MEANS' named entity 'TREATMENT' named entity 'CONCENTRATIONS' named entity 'UP-REGULATION' named entity 'MRNA' named entity 'CYCLOHEXIMIDE' named entity 'TREATED' named entity 'HL-60 CELLS' named entity 'CD13' named entity 'ACT' named entity 'INTERFERON' named entity 'ACTIVITY' named entity 'LIGANDS' named entity 'DUE TO' named entity 'CYTOKINES' covid:arg/1ea5771f0aa7465576db06da2d161c0babd17cce named entity 'BOUND' named entity 'PEPTIDES' named entity 'TGF' named entity 'ITS' named entity 'DELAYED' named entity 'TRANSDUCING' named entity 'RECENTLY' named entity 'HAVE' named entity 'IFNG' named entity 'SIGNALS' named entity 'BLOCKING ANTIBODY' named entity 'ROLES' named entity 'ENZYME ACTIVITY' named entity 'REACHING' named entity 'CHEMOKINES' named entity 'FUNCTIONS' named entity 'USING' named entity 'T-CELL' named entity 'ROLE' named entity 'COMPLETELY' named entity 'PROTEIN SYNTHESIS' named entity 'DOWN-REGULATION' named entity 'PRECEDED' named entity 'OBSERVED'

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