About: Cloning and Sequence Analysis of the Nucleocapsid Gene of Porcine Epidemic Diarrhea Virus Chinju99

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About: Cloning and Sequence Analysis of the Nucleocapsid Gene of Porcine Epidemic Diarrhea Virus Chinju99 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Cloning and Sequence Analysis of the Nucleocapsid Gene of Porcine Epidemic Diarrhea Virus Chinju99
Creator	Yeo, Lee, Hee-Kyung Sang-Geon, &
Source	PMC
abstract	The nucleocapsid (N) gene of the porcine epidemic diarrhea virus (PEDV) Chinju99 which was previously isolated in Chinju, Korea was cloned and sequenced to establish the information for the development of genetically engineered diagnostic reagents. Also, sequences of the nucleotides and deduced amino acids of the Chinju99 N gene were analyzed by alignment with those of CV777 and Br1/87. The nucleotide sequence encoding the entire N gene open reading frame (ORF) of Chinju99 was 1326 bases long and encoded a protein of 441 amino acids with predicted M (r) of 49 kDa. It consisted of 405 adenine (30.5%), 293 cytosine (22.1%), 334 guanines (25.2%) and 294 thymines (22.2%) residues. The Chinju99 N ORF nucleotide sequence was 96.5% and 96.4% homologous with that of the CV777 and Br1/87, respectively. The Chinju99 N protein revealed 96.8% amino acid identity with that of Br1/87 and CV777, respectively. The amino acid sequence contained seven potential sites for threonine (T)- or serine (S)-linked phosphorylation by each protein kinase C and casein kinase II.
has issue date	2003-01-01(xsd:dateTime)
bibo:doi	10.1023/a:1023447732567
bibo:pmid	12803473
has license	no-cc
sha1sum (hex)	1ec99dbd97c91ef0df27fe4104678405d91c1bc6
schema:url	https://doi.org/10.1023/a:1023447732567
resource representing a document's title	Cloning and Sequence Analysis of the Nucleocapsid Gene of Porcine Epidemic Diarrhea Virus Chinju99
has PubMed Central identifier	PMC7089008
has PubMed identifier	12803473
schema:publication	Virus Genes
resource representing a document's body	covid:1ec99dbd97c91ef0df27fe4104678405d91c1bc6#body_text
is schema:about of	named entity 'predicted' named entity 'reagents' named entity 'nucleocapsid' named entity 'encoded' named entity 'PHOSPHORYLATION' named entity '293' named entity 'ALIGNMENT' named entity 'POTENTIAL' named entity 'N PROTEIN' named entity 'GENE' named entity 'PREDICTED' named entity 'ADENINE' named entity 'N GENE' named entity 'BASES' named entity '334' named entity '96.5' named entity 'ENTIRE' named entity 'SEQUENCE ANALYSIS' named entity 'AMINO ACID' named entity 'ISOLATED' named entity 'ESTABLISH' named entity '405' named entity 'CLONED' named entity 'SERINE' named entity 'KOREA' named entity 'KDA' named entity 'A PROTEIN' named entity 'NUCLEOTIDE SEQUENCE' named entity 'NUCLEOCAPSID' named entity 'DIAGNOSTIC' named entity 'HOMOLOGOUS' named entity 'SEQUENCE ENCODING' named entity 'AMINO ACIDS' named entity 'SITES' named entity 'INFORMATION' named entity 'SEQUENCES' named entity '28S' named entity 'CASEIN KINASE II' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'NUCLEOCAPSID' named entity 'CLONING' named entity 'GENE' named entity 'PROTEIN ' named entity 'THREONINE' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'NUCLEOTIDES' named entity 'M R' named entity 'LONG' named entity 'REAGENTS' named entity 'REVEALED' named entity 'GENETICALLY ENGINEERED' named entity 'OPEN READING FRAME' named entity 'CYTOSINE' named entity 'ANALYZED' named entity 'PROTEIN KINASE C' named entity 'ORF' named entity 'SEVEN' named entity 'DEVELOPMENT' named entity 'ENCODED' named entity 'CONTAINED' named entity 'AMINO ACID SEQUENCE' named entity 'IDENTITY' named entity 'PREVIOUSLY' covid:arg/1ec99dbd97c91ef0df27fe4104678405d91c1bc6 named entity 'long' named entity 'protein' named entity 'serine' named entity 'Korea' named entity 'bases' named entity 'protein' named entity 'Chinju' named entity 'kDa' named entity 'Sequence' named entity 'Korea' named entity 'amino acids' named entity 'nucleotide sequence' named entity 'porcine epidemic diarrhea virus' named entity 'gene' named entity 'gene'

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