About: Molecular characterization of a Chinese vaccine strain of transmissible gastroenteritis virus: mutations that may contribute to attenuation

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About: Molecular characterization of a Chinese vaccine strain of transmissible gastroenteritis virus: mutations that may contribute to attenuation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Molecular characterization of a Chinese vaccine strain of transmissible gastroenteritis virus: mutations that may contribute to attenuation
Creator	Liu, Shengwang Shi, Hongyan Wang, Chengbao Liu, Changming Xue, Fei Qiu, Huaji Chen, • Fernando, • Li, Enjuanes Luis, Almazán Zhu, Yuanmao
source	Medline; PMC
abstract	Transmissible gastroenteritis virus (TGEV), the etiological agent of transmissible gastroenteritis (TGE), is the major cause of viral enteritis and fetal diarrhea in swine neonates, resulting in significant economic losses to the swine industry. The Chinese vaccine strain H165 of TGEV was derived from a virulent field strain H16 by serial passage in vitro. Strain H165 has been proven to be safe in piglets and pregnant sows and displays efficacy against TGEV infection. In this study, we report the complete genome sequences of strains H165 and H16, obtained by sequencing several overlapping fragments amplified from viral RNA and our findings from sequence and phylogenetic analyses. The genomes were 28,569 nucleotides in length, including the poly (A) tail. No deletions or insertions were detected in the H16 genome sequence after continuous passage in vitro; however, we found 27 nucleotide mutations in strain H165 compared with strain H16, resulting in 16 amino acid changes distributed among the genes 1, S, 3, and sM. An A to G nucleotide mutation was found in the intergenic region between the 3a and 3b genes. Furthermore, six unique nucleotides identified in the genome sequence of H165 could be used as makers to differentiate the H165 vaccine strain from wild-type TGEV strains. Our findings from phylogenetic analysis may enhance our understanding of the evolution of TGEV, as well as the other coronaviruses.
has issue date	2010-03-13(xsd:dateTime)
bibo:doi	10.1007/s11262-010-0467-6
bibo:pmid	20229183
has license	no-cc
sha1sum (hex)	22060d8bc544aa2b2d5ed5e85159ee62410b139e
schema:url	https://doi.org/10.1007/s11262-010-0467-6
resource representing a document's title	Molecular characterization of a Chinese vaccine strain of transmissible gastroenteritis virus: mutations that may contribute to attenuation
has PubMed Central identifier	PMC7088588
has PubMed identifier	20229183
schema:publication	Virus Genes
resource representing a document's body	covid:22060d8bc544aa2b2d5ed5e85159ee62410b139e#body_text
is schema:about of	named entity 'transmissible' named entity 'swine' named entity 'wild-type' named entity 'virulent' named entity 'strain' named entity 'gastroenteritis' named entity 'EFFICACY' named entity 'USED' named entity 'CAUSE' named entity 'OVERLAPPING' named entity 'CHINESE' named entity 'SWINE' named entity 'CONTINUOUS' named entity 'FOUND' named entity 'FIELD' named entity 'GENOME SEQUENCE' named entity 'WILD-TYPE' named entity 'RESULTING IN' named entity 'DETECTED' named entity 'IN VITRO' named entity 'NUCLEOTIDE' named entity 'GENES' named entity 'GENOMES' named entity 'FETAL' named entity 'GENOME' named entity 'DIFFERENTIATE' named entity 'CORONAVIRUSES' named entity 'LENGTH' named entity 'SIGNIFICANT' named entity 'COMPLETE' named entity 'VIRAL ENTERITIS' named entity 'PASSAGE' named entity 'POLY' named entity 'TAIL' named entity 'NUCLEOTIDE MUTATION' named entity 'STUDY' named entity 'VIRULENT' named entity 'UNDERSTANDING' named entity 'FINDINGS' named entity 'NUCLEOTIDES' named entity 'COULD BE' named entity 'INTERGENIC REGION' named entity 'VACCINE STRAIN' named entity 'FRAGMENTS' named entity 'DERIVED' named entity 'REPORT' named entity 'UNIQUE' named entity 'SAFE' named entity 'DISTRIBUTED' named entity 'VIRAL RNA' named entity 'AMPLIFIED' named entity 'EVOLUTION' named entity 'INDUSTRY' named entity 'AMINO ACID' named entity '28A' named entity 'MUTATIONS' named entity 'INSERTIONS' named entity 'PHYLOGENETIC ANALYSIS' named entity 'NEONATES' named entity 'ENHANCE' named entity 'OBTAINED BY' named entity 'CONTRIBUTE ' named entity 'VACCINE STRAIN' named entity 'MUTATIONS' named entity 'PROVEN' named entity 'STRAIN' named entity 'SEQUENCING' named entity 'SERIAL PASSAGE' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'MAKERS' named entity 'SEQUENCES'

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