About: PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene
Creator	Chen, Yi-Ning Chien, Chang Lin, Ababneh, Jianzhong Akin, Aydemir Cao, Wan-Jung Chen, Ching Loa, Mustafa Wu, Tsang
Source	PMC
abstract	Turkey coronaviral enteritis caused by turkey coronavirus (TCoV) continues to infect turkey flocks, resulting in significant economic loss. Determining and understanding genetic relationships among different TCoV isolates or strains is important for controlling the disease. Using two-step RT-PCR assays that amplify the full length of TCoV spike (S) gene, TCoV isolates can be sequenced, analyzed, and genotyped. Described in this chapter is the protocol on PCR amplification and sequencing analysis of full-length TCoV S gene. Such protocol is useful in molecular epidemiology for establishing an effective strategy to control the transmission of TCoV among turkey flocks.
has issue date	2015-09-10(xsd:dateTime)
bibo:doi	10.1007/978-1-4939-3414-0_14
has license	no-cc
sha1sum (hex)	221120f30c3ae1ffd34c066850c5ec003131611b
schema:url	https://doi.org/10.1007/978-1-4939-3414-0_14
resource representing a document's title	PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene
has PubMed Central identifier	PMC7122697
schema:publication	Animal Coronaviruses
resource representing a document's body	covid:221120f30c3ae1ffd34c066850c5ec003131611b#body_text
is schema:about of	named entity 'genotyped' named entity 'sequenced' named entity 'cation' named entity 'DESCRIBED' named entity 'EFFECTIVE' named entity 'ECONOMIC LOSS' named entity 'SEQUENCING ANALYSIS' named entity 'RELATIONSHIPS' covid:arg/221120f30c3ae1ffd34c066850c5ec003131611b named entity 'IMPORTANT' named entity 'PCR' named entity 'CHAPTER' named entity 'USEFUL' named entity 'STRATEGY' named entity 'RESULTING IN' named entity 'CANT' named entity 'RT-PCR' named entity 'GENETIC' named entity 'SPIKE' named entity 'CAUSED BY' named entity 'GENE' named entity 'CATION' named entity 'TO CONTROL' named entity 'TO INFECT' named entity 'TURKEY CORONAVIRUS' named entity 'PROTOCOL' named entity 'DISEASE' named entity 'ANALYZED' named entity 'MOLECULAR EPIDEMIOLOGY' named entity 'ESTABLISHING' named entity 'CHAPTER' named entity 'CATION' named entity 'UNDERSTANDING' named entity 'SEQUENCING ANALYSIS' named entity 'USING' named entity 'STEP' named entity '28S' named entity 'TRANSMISSION' named entity 'AMPLIFY' named entity 'CONTROLLING' named entity 'DIFFERENT' named entity 'ENTERITIS' named entity 'LENGTH' named entity 'STRAINS' named entity 'TURKEY' named entity 'ISOLATES' named entity 'PCR' named entity 'GENE' named entity 'LENGTH' named entity 'SPIKE' named entity 'TURKEY CORONAVIRUS' named entity 'strategy' named entity 'caused' named entity 'transmission' named entity 'Turkey' named entity 'economic loss' named entity 'TCoV' named entity 'TCoV' named entity 'Turkey' named entity 'molecular epidemiology' named entity 'PCR' named entity 'TCoV' named entity 'PCR' named entity 'TCoV' named entity 'RNA' named entity 'TCoV' named entity 'Clustal' named entity 'Protein' named entity 'nucleotide sequences' named entity 'genomics' named entity 'frame shifts' named entity 'TCoV' named entity 'DNAstar' named entity 'magnetic stirrer' named entity 'turkey'

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