About: Nonstructural protein 6 of porcine epidemic diarrhea virus induces autophagy to promote viral replication via the PI3K/Akt/mTOR axis

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About: Nonstructural protein 6 of porcine epidemic diarrhea virus induces autophagy to promote viral replication via the PI3K/Akt/mTOR axis Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Nonstructural protein 6 of porcine epidemic diarrhea virus induces autophagy to promote viral replication via the PI3K/Akt/mTOR axis
Creator	He, Kongwang Li, Bin Fan, Hongjie Lin, Huixing Zhou, Hong Liu, Mingxing
Source	Elsevier; Medline; PMC
abstract	Abstract Porcine epidemic diarrhea virus (PEDV) has caused, and continues to cause, severe economic losses to the swine industry worldwide. The pathogenic mechanism and immune regulatory interactions between PEDV and the host remain largely unknown. In this study, the interaction between autophagy and PEDV replication in intestinal porcine epithelial (IPEC-J2) cells was investigated. The effects of the structural and nonstructural proteins of PEDV on the autophagy process and the autophagy-related signaling pathways were also examined. The results shown that PEDV replication increased the autophagy flux in IPEC-J2 cells, and that autophagy was beneficial to PEDV replication, which may be one of the reasons for the rapid damage to intestinal epithelial cells and the enhanced virulence of PEDV in both newborn piglets and finishing pigs. When autophagy was pharmacologically induced by rapamycin, PEDV replication increased from 8.5 × 105 TCID50/mL to 8.8 × 106 TCID50/mL in IPEC-J2 cells. When autophagy was pharmacologically suppressed by hydroxychloroquine, PEDV replication decreased from 8.5 × 105 TCID50/mL to 7.9 × 104 TCID50/mL. To identify which PEDV proteins were the key inducers of autophagy, all 4 structural proteins and 17 nonstructural proteins of PEDV were eukaryotic expressed. It was found that the nonstructural protein 6 (nsp6) and ORF3 of PEDV were able to induce significant autophagy in IPEC-J2 cells, but the other proteins were unable to induce autophagy. It was indicated that nsp6-induced autophagy mainly occurred via the PI3K/Akt/mTOR signaling pathway. The results accelerate the understanding of the biology and pathogenesis of PEDV infection and provide new insights into the development of effective therapeutic strategies.
has issue date	2020-05-31(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2020.108684
bibo:pmid	32402351
has license	els-covid
sha1sum (hex)	22360f48da6cf571cd5d5d48a82d770e128cce2f
schema:url	https://doi.org/10.1016/j.vetmic.2020.108684
resource representing a document's title	Nonstructural protein 6 of porcine epidemic diarrhea virus induces autophagy to promote viral replication via the PI3K/Akt/mTOR axis
has PubMed Central identifier	PMC7165116
has PubMed identifier	32402351
schema:publication	Veterinary Microbiology
resource representing a document's body	covid:22360f48da6cf571cd5d5d48a82d770e128cce2f#body_text
is schema:about of	named entity 'autophagy' named entity 'pathogenic' named entity 'losses' named entity 'cells' named entity 'pathogenesis' named entity 'pharmacologically' named entity 'viral replication' named entity 'SWINE' named entity 'TCID' named entity 'PATHOGENIC' named entity 'SEVERE' named entity 'STRUCTURAL' named entity 'VIRULENCE' named entity 'MECHANISM' named entity 'ACCELERATE' named entity 'EXAMINED' named entity 'RAPID' named entity 'ENHANCED' named entity 'NONSTRUCTURAL PROTEIN' named entity 'PIGS' named entity 'NEWBORN' named entity 'FLUX' named entity 'NEW' named entity 'INTERACTION' named entity 'FOUND' named entity 'INVESTIGATED' named entity 'PROTEIN ' named entity 'HOST' named entity 'KEY' named entity 'LARGELY' named entity 'CELLS' named entity 'AUTOPHAGY' named entity 'INTERACTIONS' named entity 'PROTEINS' named entity 'PI3K' covid:arg/22360f48da6cf571cd5d5d48a82d770e128cce2f named entity 'replication' named entity 'autophagy' named entity 'therapeutic' named entity 'autophagy' named entity 'cells' named entity 'autophagy' named entity 'proteins' named entity 'host' named entity 'caused' named entity 'beneficial' named entity 'flux' named entity 'intestinal' named entity 'cells' named entity 'protein' named entity 'Porcine epidemic diarrhea virus' named entity 'autophagy' named entity 'PEDV' named entity 'therapeutic strategies' named entity 'PEDV' named entity 'intestinal epithelial cells' named entity 'autophagy' named entity 'PI3K/Akt/mTOR' named entity 'signaling pathway' named entity 'PEDV' named entity 'porcine' named entity 'PEDV' named entity 'autophagy' named entity 'PEDV' named entity 'nsp6' named entity 'nonstructural proteins' named entity 'PI3K/Akt/mTOR' named entity 'autophagy' named entity 'autophagy' named entity 'PEDV' named entity 'PEDV' named entity '7.9' named entity 'porcine epidemic diarrhea virus' named entity 'signaling pathways' named entity 'epithelial' named entity 'nonstructural protein'

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