About: Finite cell lines of turkey sperm storage tubule cells: ultrastructure and protein analysis

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Finite cell lines of turkey sperm storage tubule cells: ultrastructure and protein analysis
Creator	Garrett, Wesley Krasnec, Katina Long, Julie Shannon, Amy Talbot, Neil
Source	PMC
abstract	Cell lines of turkey sperm storage tubule (SST) epithelial cells were established. Turkey SSTs were dissected from freshly obtained uterovaginal junction (UVJ) tissue and placed in explant culture on various substrates and media. Primary cultures of SST epithelium only survived and grew from SST explants that were cultured on inactivated Sandoz inbred strain, thioguanine- and ouabain-resistance (STO) mouse feeder-cell layers in 12% fetal bovine serum-supplemented Dulbecco's Modified Eagle Medium mixed 1:1 with F12 nutrient mixture. Three independent primary colonies gave rise to 3 finite cell lines, SST-1, -2, and -3, which were continuously cultured for 8 to 16 passages at 1:3 passage ratios over a period of 3 to 4 mo. The cells were passaged by pretreatment with Y27632 and dissociation with Accutase. The SST cells grew as tightly knit monolayers on top of the feeder cells at a slow rate (approximately 96 h doubling time) at a medium pH of approximately 6.9. Lipid vacuoles were visible by light microscopy in the cells particularly at the periphery of growth. Transmission electron microscopy revealed the cells to be a polarized epithelium with apical microvilli and to have lateral tight-junction-like unions and associated desmosomes. Numerous secretory vesicles filled the upper portion of the cells’ cytoplasm, and nuclei and other major organelles such as mitochondria, rough endoplasmic reticulum, and Golgi apparatus were distributed somewhat lower in the cytoplasm. The secretory vesicles resembled mucin secretory vesicles. Proteomic analysis by mass spectroscopy of the conditioned medium of the cells, and of the cells themselves, showed the cell lines did not secrete large amounts of any particular protein, and the analysis confirmed their epithelial character. In conclusion, the SST-derived cell lines resembled the mucus-secreting cells found in the epithelium lining the UVJ of the turkey's reproductive tract.
has issue date	2018-06-01(xsd:dateTime)
bibo:doi	10.3382/ps/pey208
bibo:pmid	29860518
has license	no-cc
sha1sum (hex)	2241347c1c37806d0a3a70b7135a63c5da0bdcff
schema:url	https://doi.org/10.3382/ps/pey208
resource representing a document's title	Finite cell lines of turkey sperm storage tubule cells: ultrastructure and protein analysis
has PubMed Central identifier	PMC7107167
has PubMed identifier	29860518
schema:publication	Poult Sci
resource representing a document's body	covid:2241347c1c37806d0a3a70b7135a63c5da0bdcff#body_text
is schema:about of	named entity 'cells' named entity 'Cell lines' named entity 'Medium' named entity 'monolayers' named entity 'top' named entity 'cell lines' named entity 'protein' covid:arg/2241347c1c37806d0a3a70b7135a63c5da0bdcff named entity 'Y27632' named entity 'dissected' named entity 'mixture' named entity 'epithelial cells' named entity 'STO' named entity 'SST' named entity 'Modified' named entity 'ratios' named entity 'feeder cells' named entity 'SST-1' named entity 'explant culture' named entity 'Turkey' named entity 'sperm storage' named entity 'cell lines' named entity 'nutrient' named entity 'epithelium' named entity 'Cell lines' named entity 'PHYSIOLOGY' named entity 'tyrosine kinases' named entity 'ng/mL' named entity 'protein' named entity 'mass spectroscopy' named entity 'estrogen receptor' named entity 'epithelium' named entity 'sperm' named entity 'DMEM' named entity 'tissue repair' named entity 'zona pellucida' named entity 'Clusterin' named entity 'oxygen' named entity 'cell lines' named entity 'morphological features' named entity 'progesterone' named entity 'DMEM' named entity 'polymerized' named entity 'cytoplasmic' named entity 'cell line' named entity 'artificial insemination' named entity 'San Diego' named entity 'primary culture' named entity 'chicken' named entity 'chicken' named entity 'human embryonic stem cells' named entity 'DMEM' named entity 'Beltsville' named entity 'progesterone receptor' named entity 'extracellular vesicles' named entity 'matrix metalloproteinase' named entity 'protein' named entity 'collagenase' named entity 'protein' named entity 'reproductive tissue' named entity 'dissection' named entity 'shell gland' named entity 'mucosa' named entity 'ciliated cells' named entity 'feeder cells' named entity 'Rockville, MD' named entity 'EDTA' named entity 'staining' named entity 'feeder cells' named entity 'photomicroscopy' named entity 'body weight' named entity 'serological' named entity 'Tissue samples' named entity 'fibroblasts' named entity 'cell cultures' named entity 'feeder cells' named entity 'Applied Biosystems'

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