About: Diagnostic Reverse-Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories

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About: Diagnostic Reverse-Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Diagnostic Reverse-Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories
Creator	Drosten, Christian Eickmann, Markus Panning, Marcus Becker, Stephan Brown, David Meyer, Hermann Gopal, Robin Laue, Thomas Caro, Antonino Gü Nther, Stephan Courbot, Marie-Claude Kü Mmerer, Beate Lloyd, Graham Lschlager, Stephan Lundkvist, Ake Nilsson, Mikael Raith, Sabine
source	Medline; PMC
abstract	A network of European biosafety level 4 laboratories has designed the first industry-standard molecular assay for all filoviruses species, based on the strain collections of all participants. It uses 5 optimized L gene primers and 3 probes, as well as an internal control with a separate detection probe. Detection limits (probit analysis, 95% detection chance) were as follows: Zaire ebolavirus, 487 copies/mL of plasma; Sudan ebolavirus Maleo, 586 copies/mL; Sudan ebolavirus Gulu, 1128 copies/mL; Cote d'Ivoire ebolavirus, 537 copies/mL; Reston ebolavirus, 4546 copies/mL; Lake Victoria marburgvirus Musoke, 860 copies/mL; and Lake Victoria marburgvirus Ravn, 1551 copies/mL. The assay facilitates reliable detection or exclusion screening of filovirus infections.
has issue date	2007-11-15(xsd:dateTime)
bibo:doi	10.1086/520600
bibo:pmid	17940950
has license	no-cc
sha1sum (hex)	228de60eedef31b72a330186b75a7e8fa7aa8a74
schema:url	https://doi.org/10.1086/520600
resource representing a document's title	Diagnostic Reverse-Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories
has PubMed Central identifier	PMC7109892
has PubMed identifier	17940950
schema:publication	J Infect Dis
resource representing a document's body	covid:228de60eedef31b72a330186b75a7e8fa7aa8a74#body_text
is schema:about of	named entity 'FILOVIRUSES' named entity 'COLLECTIONS' named entity 'EUROPEAN' named entity 'MALEO' named entity 'DIAGNOSTIC' named entity 'ZAIRE EBOLAVIRUS' named entity 'POLYMERASE CHAIN REACTION' named entity 'STRAIN' named entity 'KIT' named entity 'BASED' named entity 'CHANCE' named entity 'SUDAN EBOLAVIRUS' named entity 'FOLLOWS' named entity 'COPIES' named entity 'DETECTION' named entity 'REVERSE-TRANSCRIPTION' named entity 'BIOSAFETY LEVEL 4' named entity 'LABORATORIES' named entity 'PLASMA' named entity 'plasma' named entity 'Sudan' named entity '95%' named entity 'Reverse-Transcription Polymerase Chain Reaction' named entity 'Europe' named entity 'probit analysis' named entity 'buccal swab' named entity 'Gabon' named entity 'genome' named entity 'binding site' named entity 'human disease' named entity 'ZEBOV' named entity 'RNA' named entity 'dNTP' named entity 'bovine serum albumin' named entity 'biosafety level 4' named entity 'plasmid' named entity 'virus' named entity 'filovirus' named entity 'Cote d'Ivoire ebolavirus' named entity 'SARS outbreak in 2003' named entity 'Mononegavirales' named entity 'diagnostic tests' named entity 'PCR' named entity 'digestion' named entity 'nucleotide' named entity 'Uige' named entity 'GenBank' named entity 'REBOV' named entity 'DNAse' named entity 'Crimean-Congo hemorrhagic fever' named entity 'virus' named entity 'filoviruses' named entity 'Cote d'Ivoire ebolavirus' named entity 'biosecurity' named entity 'Maleo' named entity 'biosecurity' named entity 'Reston ebolavirus' named entity 'SEBOV' named entity 'RNA' named entity 'gene' named entity 'filoviruses' named entity 'viral RNA' named entity 'infection' named entity 'European Union' named entity 'Angola'

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