About: Rapid discrimination of oseltamivir‐resistant 275Y and ‐susceptible 275H substitutions in the neuraminidase gene of pandemic influenza A/H1N1 2009 virus by duplex one‐step RT‐PCR assay

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About: Rapid discrimination of oseltamivir‐resistant 275Y and ‐susceptible 275H substitutions in the neuraminidase gene of pandemic influenza A/H1N1 2009 virus by duplex one‐step RT‐PCR assay Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid discrimination of oseltamivir‐resistant 275Y and ‐susceptible 275H substitutions in the neuraminidase gene of pandemic influenza A/H1N1 2009 virus by duplex one‐step RT‐PCR assay
Creator	Ujike, Makoto Takayama, Ikuyo Tashiro, Masato Obuchi, Masatsugu Odagiri, Takato Nakauchi, Mina Oba, Kunihiro Ejima, Miho Takashita, Emi Konomi, Nami
topic	covid:22c8431905fd983f1fe7d8995b96a0471b5004e1#this
Source	Medline; PMC
abstract	Pandemic influenza A/H1N1 2009 (A/H1N1pdm) virus caused significant outbreaks worldwide last year (2009). A number of oseltamivir‐resistant A/H1N1pdm viruses possessing an H275Y substitution in the neuraminidase (NA) protein were reported sporadically in several countries, including Japan, but they were sensitive to zanamivir and did not spread in the community. In this study, to monitor rapidly and simply oseltamivir‐resistant A/H1N1pdm viruses possessing H275Y, a duplex one‐step RT‐PCR assay (H275Y RT‐PCR assay) was developed based on an endpoint genotyping analysis method. H275Y RT‐PCR assay evaluated using several subtypes/types of influenza A and B viruses and other respiratory pathogenic viruses and shown to have high sensitivity and high specificity. Forty‐four clinical specimens were tested after RNA purification using the H275Y RT‐PCR assay, resulting in one clinical specimen being found to contain a virus possessing the H275Y mutation. Seventy‐three clinical isolates were then tested with the H275Y assay by using clinical isolates in the cultured supernatants of cells directly, without RNA purification, and the results were consistent with the NA sequencing. Since the H275Y RT‐PCR assay could detect the H275Y mutation in clinical isolates without RNA purification, as well as a H275Y mutated virus in clinical specimens after RNA purification, the assay was considered a powerful tool for surveillance screening of oseltamivir‐resistant A/H1N1pdm virus activity. J. Med. Virol. 83:1121–1127, 2011. © 2011 Wiley‐Liss, Inc.
has issue date	2011-05-12(xsd:dateTime)
bibo:doi	10.1002/jmv.22101
bibo:pmid	21567417
has license	no-cc
sha1sum (hex)	22c8431905fd983f1fe7d8995b96a0471b5004e1
schema:url	https://doi.org/10.1002/jmv.22101
resource representing a document's title	Rapid discrimination of oseltamivir‐resistant 275Y and ‐susceptible 275H substitutions in the neuraminidase gene of pandemic influenza A/H1N1 2009 virus by duplex one‐step RT‐PCR assay
has PubMed Central identifier	PMC7167129
has PubMed identifier	21567417
schema:publication	J Med Virol
resource representing a document's body	covid:22c8431905fd983f1fe7d8995b96a0471b5004e1#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/22c8431905fd983f1fe7d8995b96a0471b5004e1
is schema:about of	named entity 'virus' named entity 'virus' named entity 'RNA' named entity 'cultured' named entity 'number' named entity 'assay' named entity 'RNA' named entity 'virus' named entity 'group' named entity 'SCREENING' named entity 'BASED' named entity 'SPREAD' named entity 'CLINICAL SPECIMEN' named entity 'STEP' named entity 'SEVENTY' named entity 'MUTATION' named entity 'ISOLATES' named entity 'JAPAN' named entity 'SPECIFICITY' named entity 'YEAR' named entity 'DUPLEX' named entity 'JAPAN' named entity 'DISCRIMINATION' named entity 'worldwide' named entity 'H1N1' named entity 'isolates' named entity 'viruses' named entity 'activity' named entity 'isolates' named entity 'Forty-four' named entity 'isolates' named entity 'developed' named entity 'mutation' named entity 'screening' named entity 'pathogenic viruses' named entity 'Neuraminidase' named entity 'RT-PCR' named entity 'zanamivir' named entity 'virus' named entity 'protein' named entity 'oseltamivir' named entity 'viruses' named entity 'clinical specimen' named entity 'Assay' named entity 'influenza virus' named entity 'Influenza A/H1N1' named entity 'RNA' named entity 'Japan' named entity 'primers' named entity 'viral RNA' named entity 'centrifugation' named entity 'influenza A/H1N1' named entity 'detection limit' named entity 'oseltamivir' named entity 'positive controls' named entity 'viral titer' named entity 'real-time RT-PCR' named entity 'fluorescence' named entity 'conserved region' named entity 'RNA' named entity 'cross-reactivity' named entity 'RNA' named entity 'colonic carcinoma' named entity 'Basel' named entity 'H1N1' named entity 'Switzerland' named entity 'virion' named entity 'RNA' named entity 'viruses' named entity 'swine'

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