About: Continuous porcine cell lines developed from alveolar macrophages: Partial characterization and virus susceptibility

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Continuous porcine cell lines developed from alveolar macrophages: Partial characterization and virus susceptibility Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Continuous porcine cell lines developed from alveolar macrophages: Partial characterization and virus susceptibility
Creator	Babiuk, L Pasick, J Sabara, M Van Moorlehem, E Weingartl, H
topic	covid:23c24ef635a857f44216963d3529367d15e3b1cc#this
source	Elsevier; Medline; PMC
abstract	Porcine monomyeloid cell lines were established following transfection of primary porcine alveolar macrophage cultures with plasmid pSV3neo, carrying genes for neomycin resistance and SV40 large T antigen. The parental clone 3D4 exhibited a relatively rapid doubling time (25.5 h), high plating efficiency and mixed phenotype with respect to growth on a solid support. Single cell cloning of the 3D4 parent resulted in establishment of several cell lines; three of them designated 3D4/2, 3D4/21 and 3D4/31 were selected for further characterization. All three clones supported the replication of vesicular stomatitis virus (VSV), pseudorabies virus (PRV), classical swine fever virus (CSFV), swine vesicular disease virus (SVDV), swine poxvirus, African swine fever virus (ASFV), herpes simplex virus (HSV), parainfluenza virus, bovine adenovirus (BAV), vaccinia virus (VV), and porcine adenovirus (PAV). Under the conditions tested the cells did not support replication of porcine reproductive and respiratory syndrome virus (PRRSV). The swine myeloid character was confirmed for all three clones by fluorescence activated cell scanning (FACS) analysis using monoclonal antibodies 74-22-15 and DH59B, which recognize the pan-myeloid antigen cluster SWC3a. A subpopulation of each cell line was positive for nonspecific esterase activity and phagocytic activity to varying degrees depending on the media formulation. Cells from all three lines exhibited anchorage dependent growth when maintained in RPMI 1640 medium supplemented with 5–15% fetal bovine serum (FBS) and nonessential amino acids. Propagation in commercially formulated serum free media resulted in colony formation and growth in suspension. The addition of dimethyl sulfoxide (DMSO) or phorbol 12-myristate 13-acetate (PMA) to serum free media restored cell attachment. DMSO was also able to induce expression of CD14 monocyte marker in the 3D4/31 cell line maintained in FBS containing medium, as determined by FACS with monoclonal antibody CAM36A. Supplementation of RPMI medium with 10% porcine serum upregulated the expression of CD14 and induced expression of porcine macrophage markers recognized by antibodies 2B10 and 2G6 (Vet. Immunol. Immunopathol. 74 (2000) 163) in all three cell lines. The porcine myelomonocytic cell lines obtained may have a wide variety of applications in porcine virology and immunology.
has issue date	2002-06-13(xsd:dateTime)
bibo:doi	10.1016/s0166-0934(02)00085-x
bibo:pmid	12088830
has license	no-cc
sha1sum (hex)	23c24ef635a857f44216963d3529367d15e3b1cc
schema:url	https://doi.org/10.1016/s0166-0934%2802%2900085-x
resource representing a document's title	Continuous porcine cell lines developed from alveolar macrophages: Partial characterization and virus susceptibility
has PubMed Central identifier	PMC7119708
has PubMed identifier	12088830
schema:publication	J Virol Methods
resource representing a document's body	covid:23c24ef635a857f44216963d3529367d15e3b1cc#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/23c24ef635a857f44216963d3529367d15e3b1cc
is schema:about of	named entity 'CONTINUOUS' named entity 'SCANNING' named entity 'AFRICAN SWINE FEVER VIRUS' named entity 'COLONY FORMATION' named entity 'CLONE' named entity 'CELL LINES' named entity 'AMINO ACIDS' named entity 'MONOCLONAL ANTIBODY' named entity 'WIDE' named entity 'PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS' named entity 'INDUCE' named entity 'MEDIUM' named entity 'LINES' named entity 'PHORBOL 12-MYRISTATE 13-ACETATE' named entity 'NEOMYCIN' named entity 'CONTAINING' named entity 'INDUCED' named entity 'FBS' named entity 'LARGE T ANTIGEN' named entity 'EXPRESSION' named entity 'SWINE VESICULAR DISEASE VIRUS' named entity 'ADDITION' named entity 'SUPPORT' named entity 'DEGREES' named entity 'VARIETY' named entity 'ACTIVATED' named entity 'TESTED' named entity 'VARYING' named entity 'GROWTH' named entity 'CD14' named entity 'MYELOID' named entity 'parental' named entity 'clone' named entity 'replication' named entity 'esterase' named entity 'serum' named entity 'induce expression' named entity 'virology' named entity 'virus' named entity 'recognize' named entity 'swine' named entity 'vesicular stomatitis virus' named entity 'conditions' named entity 'vaccinia virus' named entity 'cell lines' covid:arg/23c24ef635a857f44216963d3529367d15e3b1cc named entity 'SUPPORTED' named entity 'PARENTAL' named entity 'MARKER' named entity 'VIROLOGY' named entity 'POSITIVE FOR' named entity 'FETAL BOVINE SERUM' named entity 'SOLID SUPPORT' named entity 'PORCINE' named entity 'DETERMINED BY' named entity 'PARENT' named entity 'VESICULAR STOMATITIS VIRUS' named entity 'SUSPENSION' named entity 'DIMETHYL SULFOXIDE' named entity 'CELLS' named entity 'CELL CLONING' named entity 'PORCINE ADENOVIRUS' named entity 'ESTABLISHMENT' named entity 'RECOGNIZE' named entity 'DID' named entity 'POXVIRUS' named entity '10%' named entity 'PARAINFLUENZA VIRUS' named entity 'FORMULATION' named entity 'PRIMARY' named entity 'OF EACH' named entity '3D4' named entity 'CELL' named entity 'CELL LINE' named entity 'IMMUNOL'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software