About: Enhanced Expression of Rabies Virus Surface G-Protein in Escherichia coli using SUMO Fusion

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About: Enhanced Expression of Rabies Virus Surface G-Protein in Escherichia coli using SUMO Fusion Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Enhanced Expression of Rabies Virus Surface G-Protein in Escherichia coli using SUMO Fusion
Creator	Tuli, Rakesh Praveen, • Singh, Ankit Verma, C Singh, Pradhyumna Srivastava, Meenal Dinesh, • Krishan, Yadav Rai, Mohan
source	Medline; PMC
abstract	Fusion systems are known to increase the expression of difficult to express recombinant proteins in soluble form to facilitate their purification. Rabies glycoprotein was also tough to express at sufficient level in soluble form in both E. coli and plant. The present work was aimed to over-express and purify this membrane protein from soluble extract of E. coli. Fusion of Small Ubiqutin like Modifier (SUMO) with rabies glycoprotein increased ~1.5 fold higher expression and ~3.0 fold solubility in comparison to non-fused in E. coli. The SUMO fusion also simplified the purification process. Previously engineered rabies glycoprotein gene in tobacco plants provides complete protection to mice, but the expression was very low for purification. Our finding demonstrated that the SUMO-fusion was useful for enhancing expression and solubility of the membrane protein and again proves to be a good alternative technology for applications in biomedical and pharmaceutical research. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10930-011-9373-6) contains supplementary material, which is available to authorized users.
has issue date	2011-12-02(xsd:dateTime)
bibo:doi	10.1007/s10930-011-9373-6
bibo:pmid	22134654
has license	no-cc
sha1sum (hex)	243f3b905e66c5dcdaa70cada61f92aa2e9c12e4
schema:url	https://doi.org/10.1007/s10930-011-9373-6
resource representing a document's title	Enhanced Expression of Rabies Virus Surface G-Protein in Escherichia coli using SUMO Fusion
has PubMed Central identifier	PMC7087916
has PubMed identifier	22134654
schema:publication	Protein J
resource representing a document's body	covid:243f3b905e66c5dcdaa70cada61f92aa2e9c12e4#body_text
is schema:about of	named entity 'RABIES VIRUS' named entity 'recombinant proteins' named entity 'membrane protein' named entity 'engineered' named entity 'level' named entity 'express' named entity 'expression' named entity 'purification' named entity 'Rabies' named entity 'USING' named entity 'FUSED' named entity 'GOOD' named entity 'APPLICATIONS' named entity 'INCREASED' named entity 'DIFFICULT' named entity 'COMPLETE' named entity 'PROTEIN ' named entity 'WORK' named entity 'FINDING' named entity 'NON-' named entity 'DEMONSTRATED' named entity 'PHARMACEUTICAL RESEARCH' named entity 'BUT' named entity 'TO INCREASE' named entity 'PLANTS' named entity 'PROVIDES' covid:arg/243f3b905e66c5dcdaa70cada61f92aa2e9c12e4 named entity 'PROTECTION' named entity 'KNOWN' named entity 'PROTEIN ' named entity 'FUSION' named entity 'MEMBRANE PROTEIN' named entity 'EXPRESSION' named entity 'SUMO' named entity 'SURFACE' named entity 'G-PROTEIN' named entity 'ESCHERICHIA COLI' named entity 'ENHANCED' named entity 'ENGINEERED' named entity 'LEVEL' named entity 'LIKE' named entity 'TOBACCO' named entity 'PROCESS' named entity 'TECHNOLOGY' named entity 'FOLD' named entity 'PURIFICATION' named entity 'RABIES' named entity 'PRESENT' named entity 'E. COLI' named entity 'FUSION' named entity 'THEIR' named entity 'MICE' named entity 'RECOMBINANT PROTEINS' named entity 'EXTRACT' named entity 'GLYCOPROTEIN' named entity 'HIGHER' named entity 'PLANT' named entity 'COMPARISON' named entity 'TOUGH' named entity '2A1' named entity 'VERY LOW' named entity 'ENHANCING' named entity 'GENE' named entity 'MODIFIER' named entity 'SYSTEMS' named entity 'EXPRESSION' named entity 'BIOMEDICAL' named entity 'USEFUL' named entity 'SOLUBLE' named entity 'EXPRESS' named entity 'SUFFICIENT' named entity 'SMALL' named entity 'TO EXPRESS'

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