About: Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics
Creator	Zhang, Ying Bu, Zhigao Wu, Jing Li, Fang Zhao, Dongming Chen, Qiubing Duan, Junyi Lei, Xinlin Lu, Shuhan Yin, Hao
Source	Medline; PMC
abstract	African swine fever virus (ASFV) is a dsDNA virus responsible for a severe, highly contagious, and lethal disease affecting both domestic and wild pigs. ASFV has brought enormous economic loss to a number of countries, and effective vaccine and therapy are still lacking. Therefore, a rapid, sensitive, and field-deployable detection of ASFV is important for disease surveillance and control. Herein, we developed a Cas12a-mediated portable paper assay to rapidly and precisely detect ASFV. We identified a robust set of crRNAs that recognized the highly conserved region of essential ASFV genes. The Cas12a-mediated detection assay showed low tolerance for mismatch mutations, and no cross-reactivity against other common swine pathogens. We further developed a paper-based assay to allow instrument-free detection of ASFV. Specifically, we applied gold nanoparticle–antibody conjugate to engineer homemade strips and combined it with Cas12a-mediated ASFV detection. This portable paper, instrument-free diagnostics, faithfully detected ASFV in swine samples, showing comparable sensitivity to the traditionally instrument-dependent qPCR method. Taking together, we developed a highly sensitive, instant, and economic Cas12a-mediated paper diagnostics of ASFV, with a great application potential for monitoring ASFV in the field.
has issue date	2020-04-07(xsd:dateTime)
bibo:doi	10.1038/s41421-020-0151-5
bibo:pmid	32284877
has license	cc-by
sha1sum (hex)	24a61244c69f09bc9392bbbb4a97e881ee68dc5e
schema:url	https://doi.org/10.1038/s41421-020-0151-5
resource representing a document's title	Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics
has PubMed Central identifier	PMC7136273
has PubMed identifier	32284877
schema:publication	Cell Discov
resource representing a document's body	covid:24a61244c69f09bc9392bbbb4a97e881ee68dc5e#body_text
is schema:about of	named entity 'virus' named entity 'homemade' named entity 'rapidly' named entity 'effective' named entity 'paper' named entity 'detection' named entity 'PIGS' named entity 'FREE' named entity 'SET' named entity 'ECONOMIC' named entity 'MEDIATED' named entity 'IS A' named entity 'ANTIBODY CONJUGATE' named entity 'MISMATCH' named entity 'COMBINED' named entity 'SENSITIVE' named entity 'IMPORTANT' named entity 'HIGHLY SENSITIVE' named entity 'CROSS' named entity 'SEVERE' named entity 'LETHAL' named entity 'economic' named entity 'assay' named entity 'disease' named entity 'assay' named entity 'The' named entity 'paper' named entity 'paper' named entity 'Cell' named entity 'ASFV' named entity 'virus' named entity 'assay' named entity 'vaccine' named entity 'Cas12a' named entity 'gold nanoparticle' named entity 'ASFV' named entity 'ASFV' named entity 'ASFV' named entity 'disease surveillance' named entity 'Cas12a' named entity 'antibody' named entity 'ASFV' named entity 'ASF' named entity 'DNA polymerase' named entity '30 million' named entity 'PCR' named entity 'FAT' named entity 'fluorescent' named entity 'detection limit' named entity 'RPA' named entity 'ASFV' named entity 'PCR' named entity '37°C' named entity 'PCR amplification' named entity 'digestion' named entity 'DNA polymerase' named entity 'false-positive result' named entity 'gold standard' named entity 'ASFV' named entity 'pathogens' named entity 'crispr' named entity 'ASFV' named entity 'nucleotide' named entity 'CRISPR' named entity 'qPCR' named entity 'ASFV' named entity 'Laboratory Animal' named entity 'pathogens' named entity 'positive or negative' named entity 'primers' named entity 'centrifuge' named entity 'virus'

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