About: Functional analysis of the N-terminal basic motif of a eukaryotic satellite RNA virus capsid protein in replication and packaging

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About: Functional analysis of the N-terminal basic motif of a eukaryotic satellite RNA virus capsid protein in replication and packaging Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Functional analysis of the N-terminal basic motif of a eukaryotic satellite RNA virus capsid protein in replication and packaging
Creator	Erdemci-Tandogan, Gonca Garmann, Rees Mathews, Deborah Sivanandam, Venkatesh Zandi, Roya Rao, A John, Harvard Paulson, A
Source	PMC
abstract	Efficient replication and assembly of virus particles are integral to the establishment of infection. In addition to the primary role of the capsid protein (CP) in encapsidating the RNA progeny, experimental evidence on positive sense single-stranded RNA viruses suggests that the CP also regulates RNA synthesis. Here, we demonstrate that replication of Satellite tobacco mosaic virus (STMV) is controlled by the cooperative interaction between STMV CP and the helper virus (HV) Tobacco mosaic virus (TMV) replicase. We identified that the STMV CP-HV replicase interaction requires a positively charged residue at the third position (3R) in the N-terminal 13 amino acid (aa) motif. Far-Northwestern blotting showed that STMV CP promotes binding between HV-replicase and STMV RNA. An STMV CP variant having an arginine to alanine substitution at position 3 in the N-terminal 13aa motif abolished replicase-CP binding. The N-terminal 13aa motif of the CP bearing alanine substitutions for positively charged residues located at positions 5, 7, 10 and 11 are defective in packaging full-length STMV, but can package a truncated STMV RNA lacking the 3′ terminal 150 nt region. These findings provide insights into the mechanism underlying the regulation of STMV replication and packaging.
has issue date	2016-05-19(xsd:dateTime)
bibo:doi	10.1038/srep26328
bibo:pmid	27193742
has license	cc-by
sha1sum (hex)	24f49539fad81e8f8b10650eb9cbfd60c8ee23f5
schema:url	https://doi.org/10.1038/srep26328
resource representing a document's title	Functional analysis of the N-terminal basic motif of a eukaryotic satellite RNA virus capsid protein in replication and packaging
has PubMed Central identifier	PMC4872054
has PubMed identifier	27193742
schema:publication	Sci Rep
resource representing a document's body	covid:24f49539fad81e8f8b10650eb9cbfd60c8ee23f5#body_text
is schema:about of	named entity 'SUBSTITUTION' named entity 'VARIANT' named entity 'RESIDUE' named entity 'ABOLISHED' named entity 'LACKING' named entity 'FAR' named entity 'MECHANISM' named entity 'IDENTIFIED' named entity 'RNA' named entity 'REGION' named entity 'PROTEIN ' named entity 'arginine' named entity 'RNA' named entity 'PROVIDE' named entity 'PACKAGING' named entity 'REPLICATION' named entity 'LENGTH' named entity 'BUT' named entity 'N-TERMINAL ' named entity 'BLOTTING' named entity 'ALANINE' named entity 'FINDINGS' named entity 'INSIGHTS' named entity 'REPLICASE' named entity 'PACKAGING' covid:arg/24f49539fad81e8f8b10650eb9cbfd60c8ee23f5 named entity 'SATELLITE RNA' named entity 'CAPSID PROTEIN' named entity 'EUKARYOTIC' named entity 'BASIC' named entity 'REPLICATION' named entity 'MOTIF' named entity 'FUNCTIONAL ANALYSIS' named entity 'PACKAGE' named entity 'NORTHWESTERN' named entity 'POSITION' named entity 'DEFECTIVE' named entity 'TERMINAL' named entity '80%' named entity '150' named entity 'OPEN' named entity 'RNA VIRUS' named entity 'N-TERMINAL ' named entity 'INTERACTION' named entity 'BEARING' named entity 'ARGININE' named entity 'MOTIF' named entity 'POSITIVELY CHARGED' named entity 'REGULATION' named entity 'BINDING' named entity 'UNDERLYING' named entity 'TRUNCATED' named entity 'THESE' named entity 'AMINO ACID' named entity 'residues' named entity 'substitutions' named entity 'alanine' named entity 'regulation' named entity 'promotes' named entity 'capsid' named entity 'positively charged' named entity 'alanine' named entity 'N-terminal' named entity 'positively charged' named entity 'N-terminal' named entity 'RNA' named entity 'N-terminal' named entity 'eukaryotic' named entity 'Functional analysis' named entity 'motif' named entity 'alanine' named entity 'virion' named entity 'New England Biolabs' named entity 'charge density'

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