About: Development of SYBR Green real-time RT-PCR for rapid detection, quantitation and diagnosis of unclassified bovine enteric calicivirus

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About: Development of SYBR Green real-time RT-PCR for rapid detection, quantitation and diagnosis of unclassified bovine enteric calicivirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development of SYBR Green real-time RT-PCR for rapid detection, quantitation and diagnosis of unclassified bovine enteric calicivirus
Creator	Cho, Kyoung-Oh Kang, Mun-Il Park, Sang-Ik Park, Su-Jin Jeong, Young-Ju Kim, Hyun-Jeong Kwon, Hyung-Jun Hosmillo, Myra Chun, Young-Hyun Shin, Dong-Jun Saif, Linda Park, Da-Hae
source	Elsevier; Medline; PMC
abstract	Unclassified bovine enteric calicivirus (BECV) is a newly recognized bovine enteric calicivirus that differs from bovine norovirus, and which causes diarrhea in the small intestines of calves. To date, methods such as real-time reverse transcription-polymerase chain reaction (RT-PCR) have not been developed for the rapid detection, quantitation and diagnosis of BECV. Presently, a BECV-specific SYBR Green real-time RT-PCR assay was evaluated and optimized. Diarrheic specimens (n = 118) collected from 2004 to 2005 were subjected to RT-PCR, nested PCR and SYBR Green real-time RT-PCR. By conventional RT-PCR and nested PCR, 9 (7.6%) and 59 (50%) samples tested positive, respectively, whereas the SYBR Green assay detected BECV in 91 (77.1%) samples. Using BECV RNA standards generated by in vitro transcription, the SYBR Green real-time RT-PCR assay sensitively detected BECV RNA to 1.1 × 10(0) copies/μl (correlation coefficiency = 0.98). The detection limits of the RT-PCR and nested PCR were 1.1 × 10(5) and 1.1 × 10(2) copies/μl, respectively. These results indicate that the SYBR Green real-time RT-PCR assay is more sensitive than conventional RT-PCR and nested PCR assays, and has potential as a reliable, reproducible, specific, sensitive and rapid tool for the detection, quantitation and diagnosis of unclassified BECV.
has issue date	2009-03-14(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2009.03.001
bibo:pmid	19442847
has license	no-cc
sha1sum (hex)	258ce44a78453f7d28e241962a197995d3e53c04
schema:url	https://doi.org/10.1016/j.jviromet.2009.03.001
resource representing a document's title	Development of SYBR Green real-time RT-PCR for rapid detection, quantitation and diagnosis of unclassified bovine enteric calicivirus
has PubMed Central identifier	PMC7119535
has PubMed identifier	19442847
schema:publication	J Virol Methods
resource representing a document's body	covid:258ce44a78453f7d28e241962a197995d3e53c04#body_text
is schema:about of	named entity 'GREEN ' named entity 'specific' named entity 'bovine' named entity 'copies' named entity 'bovine' named entity 'diagnosis' named entity 'DIAGNOSIS' named entity 'DEVELOPMENT' named entity 'SENSITIVE' named entity 'DIARRHEA' named entity 'CONVENTIONAL' named entity 'RNA' named entity 'RT-PCR ASSAY' named entity 'DIAGNOSIS' named entity 'SPECIMENS' named entity 'NESTED PCR ' named entity 'REAL-TIME RT-PCR' named entity 'BOVINE ENTERIC CALICIVIRUS' named entity 'POSITIVE' named entity 'REAL-TIME' named entity '1.1' named entity 'NOROVIRUS' named entity 'NESTED PCR' named entity 'THESE' named entity 'TOOL' named entity 'GENERATED' named entity 'EVALUATED' covid:arg/258ce44a78453f7d28e241962a197995d3e53c04 named entity 'BOVINE ENTERIC CALICIVIRUS' named entity 'RAPID' named entity 'COLLECTED' named entity 'UNCLASSIFIED' named entity 'ASSAY' named entity 'NEWLY' named entity 'USING' named entity 'GREEN ' named entity 'METHODS' named entity '287' named entity 'SYBR GREEN' named entity 'QUANTITATION' named entity '118' named entity 'IS A' named entity 'PRESENTLY' named entity 'POLYMERASE CHAIN REACTION' named entity 'UNCLASSIFIED' named entity 'BOVINE' named entity 'DETECTED' named entity 'STANDARDS' named entity 'SYBR GREEN' named entity 'REAL-TIME RT-PCR' named entity 'QUANTITATION' named entity 'RT-PCR' named entity 'RESULTS' named entity 'IN VITRO TRANSCRIPTION' named entity 'TESTED' named entity 'POTENTIAL' named entity 'HAVE' named entity 'DATE' named entity 'RAPID' named entity 'BECV' named entity 'DETECTION' named entity 'SAMPLES' named entity 'DETECTION' named entity 'PCR' named entity 'CALVES' named entity 'SMALL INTESTINES' named entity 'SPECIFIC' named entity 'COPIES' named entity 'LIMITS' named entity 'REVERSE TRANSCRIPTION'

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