About: The gene of an archaeal α-l-fucosidase is expressed by translational frameshifting

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About: The gene of an archaeal α-l-fucosidase is expressed by translational frameshifting Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The gene of an archaeal α-l-fucosidase is expressed by translational frameshifting
Creator	Benelli, Dario Cobucci-Ponzano, Beatrice Conte, Fiorella Flagiello, Angela Londei, Paola Monti, Maria Moracci, Marco Pucci, Piero Rossi, Mosè
Source	PMC
abstract	The standard rules of genetic translational decoding are altered in specific genes by different events that are globally termed recoding. In Archaea recoding has been unequivocally determined so far only for termination codon readthrough events. We study here the mechanism of expression of a gene encoding for a α-l-fucosidase from the archaeon Sulfolobus solfataricus (fucA1), which is split in two open reading frames separated by a −1 frameshifting. The expression in Escherichia coli of the wild-type split gene led to the production by frameshifting of full-length polypeptides with an efficiency of 5%. Mutations in the regulatory site where the shift takes place demonstrate that the expression in vivo occurs in a programmed way. Further, we identify a full-length product of fucA1 in S.solfataricus extracts, which translate this gene in vitro by following programmed −1 frameshifting. This is the first experimental demonstration that this kind of recoding is present in Archaea.
has issue date	2006-08-18(xsd:dateTime)
bibo:doi	10.1093/nar/gkl574
bibo:pmid	16920738
has license	cc-by-nc
sha1sum (hex)	25fd0362dfe0a62ca9ee6f9f634e24ef9a527dfc
schema:url	https://doi.org/10.1093/nar/gkl574
resource representing a document's title	The gene of an archaeal α-l-fucosidase is expressed by translational frameshifting
has PubMed Central identifier	PMC1616953
has PubMed identifier	16920738
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:25fd0362dfe0a62ca9ee6f9f634e24ef9a527dfc#body_text
is schema:about of	named entity 'TRANSLATIONAL' named entity 'ARCHAEA' named entity 'ENCODING' named entity 'IN VIVO' named entity 'FAR' named entity 'PLACE' named entity 'EXPERIMENTAL' named entity 'PRODUCT' named entity 'RULES' named entity 'SULFOLOBUS SOLFATARICUS' named entity 'PRESENT' named entity 'MECHANISM' named entity 'kind' named entity 'experimental' named entity 'programmed' named entity 'archaeal' named entity 'EXPRESSED' named entity 'KIND' named entity 'LENGTH' named entity 'GENE' named entity 'ESCHERICHIA COLI' named entity 'DEMONSTRATION' named entity 'HERE' named entity 'DIFFERENT' named entity 'GLOBALLY' named entity 'FUCOSIDASE' named entity 'EXTRACTS' named entity 'LED' named entity 'FUCA1' named entity 'ARCHAEON' named entity 'EFFICIENCY' named entity 'RECODING' named entity 'TRANSLATIONAL FRAMESHIFTING' named entity 'FUCOSIDASE' named entity 'EVENTS' named entity 'DETERMINED' named entity 'DECODING' named entity 'FOLLOWING' named entity 'STANDARD' named entity 'A GENE' named entity 'TRANSLATE' named entity 'SHIFT' named entity 'STUDY' named entity 'ALTERED' named entity 'SITE' named entity 'GENETIC' named entity 'GENES' named entity 'SEPARATED' named entity 'MUTATIONS' named entity 'GENE' named entity 'PRODUCTION' named entity 'SPLIT' named entity 'WHERE' named entity 'ARCHAEA ' named entity 'IN VITRO' named entity 'WAY' named entity 'SPECIFIC' named entity 'WILD-TYPE' named entity 'READTHROUGH' named entity 'TERMINATION CODON' named entity 'IDENTIFY' named entity 'EXPRESSION' named entity 'IN S' named entity 'POLYPEPTIDES' named entity 'REGULATORY' named entity 'OPEN READING FRAMES' named entity 'demonstrate' named entity 'events' named entity 'polypeptides' named entity 'regulatory site' named entity 'identify' named entity 'gene' named entity 'split gene'

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