About: A rapid point of care immunoswab assay for SARS-CoV detection

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About: A rapid point of care immunoswab assay for SARS-CoV detection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A rapid point of care immunoswab assay for SARS-CoV detection
Creator	King, Malcolm Suresh, Mavanur Das, Dipankar Kammila, Sriram Zayas-Zamora, Gustavo Bhatnagar, Pravin Sunwoo, Hoon
Source	Elsevier; Medline; PMC
abstract	The emergence of severe acute respiratory syndrome (SARS) resulted in several outbreaks worldwide. Early tests for diagnosis were not always conclusive in identifying a SARS suspected patient. Nucleocapsid protein (NP) is the most predominant virus derived structural protein which is shed in high amounts in serum and nasopharyngeal aspirate during the first week of infection. As part of such efforts, a simple, easy to use immunoswab method was developed by generating a panel of monoclonal antibodies (MAbs), Bispecific MAbs and chicken polyclonal IgY antibody against the SARS-CoV nucleocapsid protein (NP). Employing the MAb-based immunoswab, an NP concentration of 200 pg/mL in saline and pig nasopharyngeal aspirate, and 500 pg/mL in rabbit serum were detected. BsMAb-based immunoswabs detected an NP concentration of 20 pg/mL in saline, 500 pg/mL in rabbit serum and 20–200 pg/mL in pig nasopharyngeal aspirate. Polyclonal IgY-based immunoswabs detected an NP concentration of 10 pg/mL in pig nasopharyngeal aspirate providing the most sensitive SARS point of care assay. Results show that the robust immunoswab method of detecting SARS-CoV NP antigen can be developed into an easy and effective way of identifying SARS suspected individuals during a future SARS epidemic, thereby reducing and containing the transmission. The key feature of this simple immunoswab diagnostic assay is its ability to detect the presence of the SARS-CoV antigen within 45–60 min with the availability of the body fluid samples.
has issue date	2008-09-01(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2008.05.023
bibo:pmid	18620761
has license	bronze-oa
sha1sum (hex)	263a7b18731ba3b3ad0ea2450888fc28d24b77b2
schema:url	https://doi.org/10.1016/j.jviromet.2008.05.023
resource representing a document's title	A rapid point of care immunoswab assay for SARS-CoV detection
has PubMed Central identifier	PMC2678951
has PubMed identifier	18620761
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:263a7b18731ba3b3ad0ea2450888fc28d24b77b2#body_text
is schema:about of	named entity 'ability' named entity 'future' named entity 'sensitive' named entity 'detect' named entity 'assay' named entity 'SARS-CoV' named entity 'nasopharyngeal' named entity 'emergence' named entity 'serum' named entity 'DETECTED' named entity 'CONCLUSIVE' named entity 'HIGH' named entity '200' named entity 'NUCLEOCAPSID PROTEIN' named entity 'IGY' named entity 'STRUCTURAL PROTEIN' named entity 'METHOD' named entity 'MIN' named entity 'AVAILABILITY OF' named entity 'BODY FLUID' named entity 'SENSITIVE' named entity 'ANTIGEN' named entity 'EMERGENCE' named entity 'DETECTING' named entity 'AMOUNTS' named entity 'OUTBREAKS' named entity 'SARS' named entity 'SERUM' named entity 'DETECTION' named entity 'USE' named entity 'PROVIDING' named entity 'PRESENCE OF' covid:arg/263a7b18731ba3b3ad0ea2450888fc28d24b77b2 named entity 'POINT OF CARE' named entity 'SARS-COV' named entity 'SARS-COV' named entity 'POLYCLONAL' named entity 'PART OF' named entity 'TRANSMISSION' named entity 'ASSAY' named entity 'FEATURE' named entity 'WORLDWIDE' named entity 'SIMPLE' named entity 'FUTURE' named entity 'RESULTS' named entity 'SAMPLES' named entity 'WEEK' named entity 'IDENTIFYING' named entity 'PREDOMINANT' named entity 'KEY' named entity 'BASED' named entity 'EPIDEMIC' named entity 'DIAGNOSTIC ASSAY' named entity 'CONTAINING' named entity 'INFECTION' named entity 'SALINE' named entity 'CONCENTRATION OF' named entity 'NASOPHARYNGEAL ASPIRATE' named entity '500' named entity 'TO DETECT' named entity 'PIG' named entity 'FOR DIAGNOSIS' named entity 'ANTIBODY' named entity 'REDUCING' named entity 'PATIENT' named entity 'ASSAY' named entity 'GENERATING' named entity 'EMPLOYING' named entity 'PANEL' named entity 'POINT OF CARE' named entity 'MONOCLONAL ANTIBODIES' named entity 'SUSPECTED' named entity 'ROBUST' named entity 'CHICKEN' named entity 'EASY'

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