About: A new nidovirus (NamDinh virus NDiV): Its ultrastructural characterization in the C6/36 mosquito cell line

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About: A new nidovirus (NamDinh virus NDiV): Its ultrastructural characterization in the C6/36 mosquito cell line Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A new nidovirus (NamDinh virus NDiV): Its ultrastructural characterization in the C6/36 mosquito cell line
Creator	Morita, Kouichi Benedetti, Lucio Dunia, Irene Nga, Thi Quang Huy, Tran Thuy, Nguyen
Source	Elsevier; Medline; PMC
abstract	We describe the ultrastructure of the NamDinh virus (NDiV), a new member of the order Nidovirales grown in the C6/36 mosquito cell line. Uninfected and NDiV-infected cells were investigated by electron microscopy 24–48 h after infection. The results show that the viral nucleocapsid-like particles form clusters concentrated in the vacuoles, the endoplasmic reticulum, and are scattered in the cytoplasm. Mature virions of NDiV were released as budding particles on the cell surface where viral components appear to lie beneath and along the plasma membrane. Free homogeneous virus particles were obtained by ultracentrifugation on sucrose gradients of culture fluids. The size of the round-shaped particles with a complete internal structure was 80 nm in diameter. This is the first study to provide information on the morphogenesis and ultrastructure of the first insect nidovirus NDiV, a missing evolutionary link in the emergence of the viruses with the largest RNA genomes.
has issue date	2013-07-25(xsd:dateTime)
bibo:doi	10.1016/j.virol.2013.06.030
bibo:pmid	23891522
has license	no-cc
sha1sum (hex)	26dbf6c427e7d30a9ee897a91e1949f8cf54403c
schema:url	https://doi.org/10.1016/j.virol.2013.06.030
resource representing a document's title	A new nidovirus (NamDinh virus NDiV): Its ultrastructural characterization in the C6/36 mosquito cell line
has PubMed Central identifier	PMC7111958
has PubMed identifier	23891522
schema:publication	Virology
resource representing a document's body	covid:26dbf6c427e7d30a9ee897a91e1949f8cf54403c#body_text
is schema:about of	named entity 'homogeneous' named entity 'CHARACTERIZATION' named entity 'CONCENTRATED' named entity 'INVESTIGATED' named entity 'ULTRACENTRIFUGATION' named entity 'UNINFECTED' named entity 'ROUND' named entity 'SUCROSE' named entity 'INFORMATION' named entity 'ELECTRON MICROSCOPY' named entity 'MISSING' named entity 'LARGEST' named entity 'FORM' named entity 'RNA' named entity 'VACUOLES' named entity 'FREE' named entity 'BUDDING' named entity 'GRADIENTS' named entity 'MEMBER OF' named entity 'SHAPED' named entity 'OBTAINED BY' named entity 'COMPLETE' named entity 'VIRIONS' named entity 'PARTICLES' named entity 'ITS' named entity 'VIRUS' named entity 'EMERGENCE' named entity 'CELLS' named entity 'STRUCTURE' named entity 'FLUIDS' named entity 'CELL LINE' named entity 'MOSQUITO' named entity 'NEW' named entity 'INSECT' named entity 'NEW' named entity 'INFECTED' named entity 'DIAMETER' named entity 'CULTURE' named entity 'ULTRASTRUCTURE' named entity 'APPEAR' named entity 'CELL LINE' named entity 'INFECTION' named entity 'VIRUSES' named entity 'WHERE' named entity 'RELEASED' named entity 'LINK' named entity 'CLUSTERS' named entity 'LIKE' named entity 'CELL SURFACE' named entity 'CYTOPLASM' named entity 'VIRAL NUCLEOCAPSID' named entity 'MATURE' named entity 'PLASMA MEMBRANE' named entity 'MOSQUITO' named entity 'DESCRIBE' named entity 'LIE' named entity 'MORPHOGENESIS' named entity 'ENDOPLASMIC RETICULUM' named entity 'STUDY' named entity 'HOMOGENEOUS' named entity 'VIRUS' named entity 'GENOMES' named entity 'SIZE' named entity 'INTERNAL' named entity 'VIRAL COMPONENTS' named entity 'ORDER NIDOVIRALES' named entity 'SCATTERED' named entity 'RESULTS' named entity 'TO PROVIDE' named entity 'BENEATH' covid:arg/26dbf6c427e7d30a9ee897a91e1949f8cf54403c named entity 'morphogenesis' named entity 'electron microscopy' named entity 'Mature' named entity 'cell' named entity 'virus'

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