About: Development and evaluation of a four-tube real time multiplex PCR assay covering fourteen respiratory viruses, and comparison to its corresponding single target counterparts

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Development and evaluation of a four-tube real time multiplex PCR assay covering fourteen respiratory viruses, and comparison to its corresponding single target counterparts Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and evaluation of a four-tube real time multiplex PCR assay covering fourteen respiratory viruses, and comparison to its corresponding single target counterparts
Creator	Beld, Marcel Koekkoek, Sylvie Molenkamp, Richard Pajkrt, Dasja Schinkel, Janke De Jong, Menno Jansen, Rogier
Source	Elsevier; Medline; PMC
abstract	BACKGROUND: Multiplex real time PCR is increasingly used to diagnose respiratory viruses and has shown to be superior to traditional methods, like culture and antigen detection. However, comprehensive data on sensitivity, specificity and performance of the multiplex PCR compared to the single target PCR's is limited for most published respiratory multiplex real time PCR assays. OBJECTIVES: Development and extensive analysis of an internally controlled multiplex real time rt-PCR for detection of respiratory viruses. STUDY DESIGN: The assay was validated in comparison to single-target PCR's using plasmid targets and prospectively collected nasopharyngeal aspirates. RESULTS: Using plasmid targets the multiplex format was found to be as least as sensitive and specific as the single-target PCR and no competition was observed when different targets were present at different amounts in one tube. Clinical validation showed high concordance for all viruses tested except for samples with low levels of enterovirus. CONCLUSION: This multiplex showed excellent specificities for all 14 respiratory viruses and sensitivity was high except for clinical samples with low levels of enterovirus.
has issue date	2011-05-14(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2011.04.010
bibo:pmid	21571585
has license	no-cc
sha1sum (hex)	26e1199f4fb9b496c1d127d03cbed9f03b406325
schema:url	https://doi.org/10.1016/j.jcv.2011.04.010
resource representing a document's title	Development and evaluation of a four-tube real time multiplex PCR assay covering fourteen respiratory viruses, and comparison to its corresponding single target counterparts
has PubMed Central identifier	PMC7108253
has PubMed identifier	21571585
schema:publication	J Clin Virol
resource representing a document's body	covid:26e1199f4fb9b496c1d127d03cbed9f03b406325#body_text
is schema:about of	named entity 'RESPIRATORY VIRUSES' covid:arg/26e1199f4fb9b496c1d127d03cbed9f03b406325 named entity 'TUBE' named entity 'REAL TIME' named entity 'RESPIRATORY' named entity 'RESPIRATORY VIRUSES' named entity 'CLINICAL VALIDATION' named entity 'HIGH' named entity 'ANTIGEN DETECTION' named entity 'TUBE' named entity 'EXCELLENT' named entity 'SPECIFIC' named entity 'PCR' named entity 'LEVELS' named entity 'VIRUSES' named entity 'CONCLUSION' named entity 'TARGETS' named entity 'EXTENSIVE' named entity 'DIFFERENT' named entity 'DEVELOPMENT' named entity 'TESTED' named entity 'PUBLISHED' named entity 'USED' named entity 'TRADITIONAL' named entity 'COLLECTED' named entity 'SINGLE' named entity 'FOUND' named entity 'TARGET' named entity 'REAL TIME PCR' named entity 'STUDY DESIGN' named entity 'OBSERVED' named entity 'EXCEPT FOR' named entity 'DATA' named entity 'LIMITED' named entity 'LOW' named entity 'ASPIRATES' named entity 'DEVELOPMENT' named entity 'SINGLE' named entity 'PERFORMANCE' named entity 'VALIDATED' named entity 'PCR ASSAY' named entity 'ITS' named entity 'MULTIPLEX PCR' named entity 'COVERING' named entity 'EVALUATION' named entity 'NASOPHARYNGEAL' named entity 'PRESENT' named entity 'COMPREHENSIVE' named entity 'METHODS' named entity 'BACKGROUND' named entity 'AMOUNTS' named entity 'USING' named entity 'TARGET' named entity 'MULTIPLEX PCR' named entity 'CONTROLLED' named entity 'RESULTS' named entity 'REAL TIME RT-PCR' named entity 'FOURTEEN' named entity 'COMPARISON' named entity 'SPECIFICITY' named entity 'SUPERIOR' named entity '27S' named entity 'COMPARISON' named entity 'CULTURE' named entity 'DIAGNOSE' named entity 'SENSITIVE' named entity 'DETECTION' named entity 'PLASMID' named entity 'ENTEROVIRUS' named entity 'LIKE' named entity 'ASSAY' named entity 'CONCORDANCE' named entity 'CLINICAL' named entity 'ANALYSIS' named entity 'SENSITIVITY'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software