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About:
Proposal of Reverse Transcription-PCR–Based Mass Population Screening for SARS-CoV-2 (COVID-19)
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schema:ScholarlyArticle
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wasabi.inria.fr
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Type:
Academic Article
research paper
schema:ScholarlyArticle
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type
Academic Article
research paper
schema:ScholarlyArticle
isDefinedBy
Covid-on-the-Web dataset
has title
Proposal of Reverse Transcription-PCR–Based Mass Population Screening for SARS-CoV-2 (COVID-19)
Creator
Mondal, A
Ahluwalia, P
Ahluwalia, M
Ananth, S
Chaubey, A
Hegde, M
Jilani, Y
Jones, K
Kolhe, R
Kota, V
Njau, A
Rojiani, A
Source
Elsevier; Medline; PMC
abstract
Testing for SARS-CoV-2 has lagged behind in many countries due to lack of adequate test kits and bottlenecks in the analytical process. The aim of this study was to investigate the feasibility and accuracy of a sample pooling approach for wide-scale population screening for COVID-19. A total of 940 nasopharyngeal-swab samples (934 negative and 6 positive) previously tested for SARS-CoV-2 were de-identified and assigned random numbers for analysis. From this, 94 pools of 10 samples each were created. Automated RNA extraction followed by RT-PCR was carried out in a 96 well plate. Positive pools were identified and the individual samples were re-analyzed.
has issue date
2020-07-30
(
xsd:dateTime
)
bibo:doi
10.1016/j.jmoldx.2020.07.001
bibo:pmid
32738298
has license
no-cc
sha1sum (hex)
27194985a827529bc5aa4a23c566e9f47bd2e5bd
schema:url
https://doi.org/10.1016/j.jmoldx.2020.07.001
resource representing a document's title
Proposal of Reverse Transcription-PCR–Based Mass Population Screening for SARS-CoV-2 (COVID-19)
has PubMed Central identifier
PMC7391280
has PubMed identifier
32738298
schema:publication
J Mol Diagn
resource representing a document's body
covid:27194985a827529bc5aa4a23c566e9f47bd2e5bd#body_text
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named entity 'tested'
named entity 'positive'
named entity 'comparable'
named entity 'plate'
named entity 'test'
named entity 'samples'
named entity 'Proposal'
named entity 'WELLS'
named entity 'SARS-COV-2'
named entity 'COMPARED'
named entity 'samples'
named entity 'individual'
named entity 'Thus'
named entity 'wells'
named entity 'SARS-CoV-2'
named entity 'SARS-CoV-2'
named entity 'test kits'
named entity 'COVID'
named entity 'PCR'
named entity 'Testing'
named entity 'asymptomatic'
named entity 'spread of disease'
named entity 'range Table'
named entity 'community spread'
named entity 'test kits'
named entity 'assay'
named entity 'incidence rate'
named entity 'health care'
named entity 'COVID-19'
named entity 'RT-PCR'
named entity 'SARS-CoV-2'
named entity 'test kits'
named entity 'LMIC'
named entity 'disease surveillance'
named entity 'short supply'
named entity 'RT-PCR'
named entity 'reagent'
named entity 'public health care'
named entity 'Food Administration'
named entity 'healthcare facilities'
named entity 'South Korea'
named entity 'COVID-19 pandemic'
named entity 'SARS-CoV-2'
named entity 'diagnostic assays'
named entity 'personal hygiene'
named entity 'RNA extraction'
named entity 'low and middle income countries'
named entity 'Wuhan'
named entity 'cost effective'
named entity 'quarantine'
named entity 'personal protective equipment'
named entity 'incidence rates'
named entity 'test kits'
named entity 'test kits'
named entity 'blood donations'
named entity 'RNA extraction'
named entity 'epidemiological data'
named entity 'SARS-CoV-2'
named entity 'quarantine'
named entity 'RNA extraction'
named entity 'FDA'
named entity 'COVID-19'
named entity 'proof-of-concept'
named entity 'quality control'
named entity 'negative result'
named entity 'tested positive'
named entity 'mortality rates'
named entity '1 million'
named entity 'community spread'
named entity 'assay'
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