About: Characterization of cytotoxic T‐lymphocyte epitopes and immune responses to SARS coronavirus spike DNA vaccine expressing the RGD‐integrin‐binding motif

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About: Characterization of cytotoxic T‐lymphocyte epitopes and immune responses to SARS coronavirus spike DNA vaccine expressing the RGD‐integrin‐binding motif Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Characterization of cytotoxic T‐lymphocyte epitopes and immune responses to SARS coronavirus spike DNA vaccine expressing the RGD‐integrin‐binding motif
Creator	Koh, D Wong, S Chow, Vincent Lu, J Phoon, M Macary, P Narasaraju, T Pereira, N Poh, W Zhong, F
source	Medline; PMC
abstract	Integrins are critical for initiating T‐cell activation events. The integrin‐binding motif Arg‐Gly‐Asp (RGD) was incorporated into the pcDNA 3.1 mammalian expression vector expressing the codon‐optimized extracellular domain of SARS coronavirus (SARS‐CoV) spike protein, and tested by immunizing C57BL/6 mice. Significant cell‐mediated immune responses were characterized by cytotoxic T‐lymphocyte (51)Cr release assay and interferon‐gamma secretion ELISPOT assay against RMA‐S target cells presenting predicted MHC class I H2‐Kb epitopes, including those spanning residues 884–891 and 1116–1123 within the S2 subunit of SARS‐CoV spike protein. DNA vaccines incorporating the Spike‐RGD/His motif or the Spike‐His construct generated robust cell‐mediated immune responses. Moreover, the Spike‐His DNA vaccine construct generated a significant antibody response. Immunization with these DNA vaccine constructs elicited significant cellular and humoral immune responses. Additional T‐cell epitopes within the SARS‐CoV spike protein that may contribute to cell‐mediated immunity in vivo were also identified. J. Med. Virol. 81:1131–1139, 2009. © 2009 Wiley‐Liss, Inc.
has issue date	2009-05-27(xsd:dateTime)
bibo:doi	10.1002/jmv.21571
bibo:pmid	19475608
has license	no-cc
sha1sum (hex)	2789bd81aa8d5c6846de5d11fe7e080c9e958ac8
schema:url	https://doi.org/10.1002/jmv.21571
resource representing a document's title	Characterization of cytotoxic T‐lymphocyte epitopes and immune responses to SARS coronavirus spike DNA vaccine expressing the RGD‐integrin‐binding motif
has PubMed Central identifier	PMC7166745
has PubMed identifier	19475608
schema:publication	J Med Virol
resource representing a document's body	covid:2789bd81aa8d5c6846de5d11fe7e080c9e958ac8#body_text
is schema:about of	named entity 'cell-mediated immune responses' named entity 'interferon-gamma' named entity 'DNA Vaccine' named entity 'Cytotoxic T-Lymphocyte' named entity 'Expressing' named entity 'TARGET CELLS' named entity 'DNA VACCINE' named entity 'SARS CORONAVIRUS' named entity 'MEDIATED' named entity 'EPITOPES' named entity 'ELICITED' named entity '891' named entity 'THESE' named entity 'motif' named entity 'expressing' named entity 'DNA vaccines' named entity 'construct' named entity 'protein' named entity 'robust' named entity 'C57BL/6' named entity 'antibody response' named entity 'expression vector' named entity 'MHC' named entity 'secretion' named entity 'DNA vaccine' named entity 'binding motif' named entity 'epitopes' named entity 'Arg' named entity 'interferon-gamma' named entity 'humoral immune responses' named entity 'target cells' named entity 'SARS' named entity 'Binding Motif' named entity 'Cytotoxic T-Lymphocyte' named entity 'cellular' named entity 'fluorescence' named entity 'Rauscher murine leukemia virus' named entity 'global outbreak' named entity 'IgG' named entity 'previously identified' named entity 'DNA vaccine' named entity 'alopecia' named entity 'antibody' named entity 'lysis' named entity 'blood cells' named entity 'HLA-A' named entity 'effector' named entity 'H-2b' named entity 'receptor' named entity 'hypervariable region' named entity 'spike protein' named entity 'receptor' named entity 'DNA vaccines' named entity 'insulin' named entity 'SARS-CoV' named entity 'primers' named entity 'epitopes' named entity 'SARS-CoV' named entity 'intramuscular injections' named entity 'peptides' named entity 'immunization' named entity 'lymph nodes' named entity 'peptide' named entity 'plasmid DNA' named entity 'transfect' named entity 'PBS' named entity '1:500' named entity 'flow cytometer' named entity 'T-cell activation' named entity 'serum' named entity 'peptide' named entity 'cellular immune responses'

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