About: Novel protein chip for the detection of antibodies against infectious bronchitis virus

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About: Novel protein chip for the detection of antibodies against infectious bronchitis virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Novel protein chip for the detection of antibodies against infectious bronchitis virus
Creator	Lei, Jing Shi, Zhiyu Yan, Liping Zhou, Jiyong Li, Hui Li, Yuan Bi, Zhenwei Chen, Yuqing Fang, An Hu, Jianhua Xiao, Qian Yao, Lu Liao, Min Song, Suquan
Source	PMC
abstract	BACKGROUND: Infectious bronchitis (IB) caused by the IB virus (IBV) can cause acute damage to chickens around the world. Therefore, rapid diagnosis and immune status determination are critical for controlling IBV outbreaks. Enzyme-linked immunosorbent assays (ELISAs) have been widely used in the detection of IBV antibodies in the early infection and continuous infection of IB because they are more sensitive and quicker than other diagnostic methods. RESULTS: We have developed two indirect microarray methods to detect antibodies against IBV: a chemiluminescent immunoassay test (CIT) and a rapid diagnostic test (RDT). IBV nonstructural protein 5 (nsp5) was expressed, purified from Escherichia coli, and used to spot the initiator integrated poly(dimethylsiloxane), which can provide a near “zero” background for serological assays. Compared with the IDEXX IBV Ab Test kit, CIT and RDT have a sensitivity and specificity of at least 98.88% and 91.67%, respectively. No cross-reaction was detected with antibodies against avian influenza virus subtypes (H5, H7, and H9), Newcastle disease virus, Marek’s disease virus, infectious bursal disease virus, and chicken anemia virus. The coefficients of variation of the reproducibility of the intra- and inter-assays for CIT ranged from 0.8 to 18.63%. The reproducibility of RDT was consistent with the original results. The application of the IBV nsp5 protein microarray showed that the positive rate of the CIT was 96.77%, that of the nsp5 ELISA was 91.40%, and that of the RDT was 90.32%. Furthermore, the RDT, which was visible to the naked eye, could be completed within 15 min. Our results indicated that compared with nsp5 ELISA, the CIT was more sensitive, and the RDT had similar positive rates but was faster. Furthermore, the two proposed methods were specific and stable. CONCLUSIONS: Two microarray assays, which were rapid, specific, sensitive, and relatively simple, were developed for the detection of an antibody against IBV. These methods can be of great value for the surveillance of pathogens and monitoring the efficiency of vaccination.
has issue date	2018-09-17(xsd:dateTime)
bibo:doi	10.1186/s12917-018-1586-x
bibo:pmid	30223836
has license	cc-by
sha1sum (hex)	27f21a68a6f074f434e506aee9492fa2c772847c
schema:url	https://doi.org/10.1186/s12917-018-1586-x
resource representing a document's title	Novel protein chip for the detection of antibodies against infectious bronchitis virus
has PubMed Central identifier	PMC6142349
has PubMed identifier	30223836
schema:publication	BMC Vet Res
resource representing a document's body	covid:27f21a68a6f074f434e506aee9492fa2c772847c#body_text
is schema:about of	named entity 'INTRA' named entity 'COMPLETED' named entity 'INFECTION' named entity 'INITIATOR' named entity 'TO DETECT' named entity 'MAREK' named entity 'DIAGNOSTIC' named entity 'VISIBLE TO' named entity 'COULD BE' named entity 'ESCHERICHIA COLI' named entity 'NSP5' named entity 'DETERMINATION' named entity 'MIN' named entity 'BACKGROUND' named entity 'PURIFIED' named entity 'CAUSE' named entity 'PROTEIN MICROARRAY' named entity 'RAPID DIAGNOSIS' named entity 'ANTIBODIES' named entity '0.8' named entity 'POSITIVE RATE' named entity 'METHODS' named entity 'DIAGNOSTIC TEST' named entity 'INDIRECT' named entity 'PROTEIN ' named entity 'IBV' named entity 'REPRODUCIBILITY' named entity 'DAMAGE' named entity 'COEFFICIENTS' named entity 'NOVEL PROTEIN' named entity 'PROTEIN CHIP' named entity 'assays' named entity 'infection' named entity 'ranged' named entity 'damage' named entity '15 min' named entity 'IBV' named entity 'Results' named entity 'Novel' named entity 'OUTBREAKS' named entity 'HAVE' named entity 'VIRUS' named entity 'SPECIFIC' named entity 'DISEASE VIRUS' named entity 'FASTER' named entity 'AVIAN INFLUENZA VIRUS' named entity 'USED' named entity 'APPLICATION' named entity 'RATES' named entity 'NAKED' named entity 'INDICATED' named entity 'EARLY' named entity 'NEAR' named entity 'OUR' named entity 'TEST KIT' named entity 'SIMILAR' named entity 'CHICKEN ANEMIA VIRUS' named entity 'SPOT' named entity 'CHICKENS' named entity 'CONSISTENT WITH' named entity 'INTER-' named entity '27S' named entity 'VARIATION' named entity 'EYE' named entity 'MICROARRAY' named entity 'INFECTIOUS BURSAL DISEASE VIRUS' named entity 'SENSITIVE' named entity 'INFECTIOUS BRONCHITIS VIRUS'

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