About: Identification of a Novel Coronavirus from Guinea Fowl Using Metagenomics

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification of a Novel Coronavirus from Guinea Fowl Using Metagenomics
Creator	Guérin, Jean-Luc Ducatez, Mariette
Source	Medline; PMC
abstract	While classical virology techniques such as virus culture, electron microscopy, or classical PCR had been unsuccessful in identifying the causative agent responsible for the fulminating disease of guinea fowl, we identified a novel avian gammacoronavirus associated with the disease using metagenomics. Next-generation sequencing is an unbiased approach that allows the sequencing of virtually all the genetic material present in a given sample.
has issue date	2014-12-18(xsd:dateTime)
bibo:doi	10.1007/978-1-4939-2438-7_2
bibo:pmid	25720467
has license	no-cc
sha1sum (hex)	27fbaea4fd3a233f1acfd04b420ac798d206b183
schema:url	https://doi.org/10.1007/978-1-4939-2438-7_2
resource representing a document's title	Identification of a Novel Coronavirus from Guinea Fowl Using Metagenomics
has PubMed Central identifier	PMC7120858
has PubMed identifier	25720467
schema:publication	Coronaviruses
resource representing a document's body	covid:27fbaea4fd3a233f1acfd04b420ac798d206b183#body_text
is schema:about of	named entity 'CATION' named entity 'SAMPLE' named entity 'GUINEA FOWL' named entity 'VIROLOGY' named entity 'VIRUS CULTURE' named entity 'ALLOWS' named entity 'USING' named entity 'PRESENT' named entity 'GUINEA FOWL' named entity 'SEQUENCING' named entity 'NOVEL CORONAVIRUS' named entity 'ELECTRON MICROSCOPY' named entity 'CHAPTER' named entity 'METAGENOMICS' named entity 'GAMMACORONAVIRUS' named entity 'GIVEN' named entity 'DISEASE' named entity 'APPROACH' named entity 'CAUSATIVE AGENT' named entity 'METAGENOMICS' named entity 'GENETIC MATERIAL' named entity 'IDENTIFYING' named entity 'ASSOCIATED WITH' named entity 'CLASSICAL' named entity 'AS VIRUS' named entity 'UNSUCCESSFUL' named entity 'NOVEL' named entity 'PCR' named entity 'RESPONSIBLE' named entity 'AVIAN' named entity 'TECHNIQUES' named entity 'USING' named entity 'genetic material' named entity 'Guinea Fowl' named entity 'room temperature' named entity 'agarose gel' named entity 'pathogen' named entity 'electronic microscopy' named entity 'ddNTP' named entity 'penicillin' named entity 'Centrifuge' named entity 'Quality assessment' named entity 'multiplexed' named entity 'PCR' named entity 'room temperature' named entity 'quantitation' named entity 'RNA viruses' named entity 'primers' named entity 'DNAse' named entity 'PBS' named entity 'gold standard' named entity 'primers' named entity 'NGS' named entity 'room temperature' named entity 'chloroform' named entity 'Incubate' named entity 'genomes' named entity 'Klenow' named entity 'TRIzol' named entity 'pol1' named entity 'DNA extraction' named entity 'poults' named entity 'SAMtools' named entity 'cation' named entity 'guinea fowl' named entity '11,000' named entity 'streptomycin' named entity 'sequencing technology' named entity 'GAAS' named entity 'gammacoronavirus' named entity 'assay' named entity 'reference genome' named entity 'pathogen' named entity 'cation' named entity 'next-generation sequencing' named entity 'PCR' named entity 'dNTP' named entity 'nucleic acids' named entity 'DNAse' named entity 'supernatant'

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