About: The Characterization of Immunoprotection Induced by a cDNA Clone Derived from the Attenuated Taiwan Porcine Epidemic Diarrhea Virus Pintung 52 Strain

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About: The Characterization of Immunoprotection Induced by a cDNA Clone Derived from the Attenuated Taiwan Porcine Epidemic Diarrhea Virus Pintung 52 Strain Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The Characterization of Immunoprotection Induced by a cDNA Clone Derived from the Attenuated Taiwan Porcine Epidemic Diarrhea Virus Pintung 52 Strain
Creator	Chang, Hui-Wen Jeng, Chian-Ren Cheng, Ivan-Chen Chang, Yen-Chen Chiou, Hue-Ying Tsai, Pei-Shiue Kao, Chi-Fei Hsueh, Cheng-Shun Pang, Victor
Source	Medline; PMC
abstract	The porcine epidemic diarrhea virus (PEDV) poses a great threat to the global swine industries and the unreliable protection induced by the currently available vaccines remains a major challenge. We previously generated a genogroup 2b (G2b) PEDV Taiwan Pintung 52 (PEDVPT) strain, PEDVPT-P96, and determined its promising host immune response against the virulent PEDVPT-P5 strain. To study the attenuation determinants of PEDVPT-P96 and establish a PEDVPT-P96-based recombinant vector as a vaccine platform for further antigenicity modification, iPEDVPT-P96, a full-length cDNA clone of PEDVPT-P96, was established. Comparing to the parental PEDVPT-P96 virus, the iPEDVPT-P96 virus showed efficient replication kinetics with a delayed decline of viral load and similar but much more uniform plaque sizes in Vero cells. In the 5-week-old piglet model, fecal viral shedding was observed in the PEDVPT-P96-inoculated piglets, whereas those inoculated with iPEDVPT-P96 showed neither detectable fecal viral shedding nor PEDV-associated clinical signs. Moreover, inoculation with iPEDVPT-P96 elicited comparable levels of anti-PEDV specific plasma IgG and fecal/salivary IgA, neutralizing antibody titers, and similar but less effective immunoprotection against the virulent PEDVPT-P5 challenge compared to the parental PEDVPT-P96. In the present study, an infectious cDNA clone of an attenuated G2b PEDV strain was successfully generated for the first time, and the in vitro and in vivo data indicate that iPEDVPT-P96 is further attenuated but remains immunogenic compared to its parental PEDVPT-P96 viral stock. The successful development of the iPEDVPT-P96 cDNA clone could allow for the manipulation of the viral genome to study viral pathogenesis and facilitate the rapid development of effective vaccines.
has issue date	2018-10-04(xsd:dateTime)
bibo:doi	10.3390/v10100543
bibo:pmid	30287770
has license	cc-by
sha1sum (hex)	2813c9a864720f35199261a3ab219750d3a5daeb
schema:url	https://doi.org/10.3390/v10100543
resource representing a document's title	The Characterization of Immunoprotection Induced by a cDNA Clone Derived from the Attenuated Taiwan Porcine Epidemic Diarrhea Virus Pintung 52 Strain
has PubMed Central identifier	PMC6213177
has PubMed identifier	30287770
schema:publication	Viruses
resource representing a document's body	covid:2813c9a864720f35199261a3ab219750d3a5daeb#body_text
is schema:about of	named entity 'cells' named entity 'antigenicity' named entity 'remains' named entity 'viral shedding' named entity 'inoculated' named entity 'fecal' named entity 'levels' named entity 'virulent' named entity 'clone' named entity 'Taiwan' covid:arg/2813c9a864720f35199261a3ab219750d3a5daeb named entity 'immunogenic' named entity 'vivo' named entity 'immune response' named entity 'virus' named entity 'recombinant' named entity 'virulent' named entity 'plasma' named entity 'porcine epidemic diarrhea virus' named entity 'vaccines' named entity 'PEDV' named entity 'virulent' named entity 'virus' named entity 'cDNA clone' named entity 'viral load' named entity 'IgA' named entity 'IgG' named entity 'virus' named entity 'viral shedding' named entity 'swine' named entity 'cDNA clone' named entity 'immunogenic' named entity 'Immunoprotection' named entity 'Clone' named entity 'stock' named entity 'ethanol' named entity 'single-stranded RNA' named entity 'G2b' named entity 'Vero' named entity 'Vero' named entity 'zoonosis' named entity 'centrifugation' named entity 'silent mutation' named entity 'assay' named entity 'complement' named entity 'recombinant' named entity 'transcription' named entity 'titer' named entity 'viral shedding' named entity 'PCR' named entity 'enzyme-linked immunosorbent assay' named entity 'Germany' named entity 'acetone' named entity 'nucleocapsid' named entity 'antigenicity' named entity 'Vero' named entity 'GTP' named entity 'restriction enzyme sites' named entity 'pathogenicity' named entity 'virion' named entity 'nucleotides' named entity 'Nidovirales' named entity 'PEDV' named entity 'cell fusion' named entity 'IgA' named entity 'virus' named entity 'viral entry' named entity 'Dulbecco's modified Eagle's medium' named entity 'nucleocapsid' named entity 'PBS' named entity 'saliva' named entity 'statistically significant' named entity 'PEDV'

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