About: Development of a Quantitative Loop-Mediated Isothermal Amplification Assay for the Rapid Detection of Novel Goose Parvovirus

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About: Development of a Quantitative Loop-Mediated Isothermal Amplification Assay for the Rapid Detection of Novel Goose Parvovirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development of a Quantitative Loop-Mediated Isothermal Amplification Assay for the Rapid Detection of Novel Goose Parvovirus
Creator	Zhu, Guoqiang Yang, Jing Chen, Hao Diao, Youxiang Niu, Xiaoyu Tang, Yi Wang, Zhenzhong Yu, Xianglong Gallardo, Rodrigo
source	PMC
abstract	An infectious disease characterized with short bills and protruding tongues has attacked to meat ducks in China since March 2015, which has caused ducks poor growth and enormous economic losses to duck industry of China. It was eventually proved to be caused by parvovirus after pathogen isolation and identification. As the genomic sequence analysis showed, this pathogen shared 90.8–94.6% of nucleotide identity with goose parvovirus (GPV), and it was called duck-origin novel goose parvovirus (N-GPV). In this study, a quantitative loop-mediated isothermal amplification (qLAMP) assay was developed for the rapid diagnosis of N-GPV. A set of four specific primers, two inner and two outer, were designed targeting at VP3 gene, which could be completed within 60 min at 65°C in water bath or on a real-time PCR instrument for quantitative analysis. Specificity test of LAMP assay showed that there was no cross-reactivity between N-GPV and other duck pathogens, and the detection limit of qLAMP assay was 1.0 × 10(2) copies/μL. The repeatability of this method was confirmed by inter-assay and intra-assay tests with variability ranging from 0.74 to 2.25%. The results have indicated that the qLAMP assay was a simple, rapid, accurate, sensitive, and specific method for detecting N-GPV, especially on field detection.
has issue date	2017-12-12(xsd:dateTime)
bibo:doi	10.3389/fmicb.2017.02472
bibo:pmid	29312182
has license	cc-by
sha1sum (hex)	283604cd89c223271768c0a2fb065cba3a015b13
schema:url	https://doi.org/10.3389/fmicb.2017.02472
resource representing a document's title	Development of a Quantitative Loop-Mediated Isothermal Amplification Assay for the Rapid Detection of Novel Goose Parvovirus
has PubMed Central identifier	PMC5732990
has PubMed identifier	29312182
schema:publication	Front Microbiol
resource representing a document's body	covid:283604cd89c223271768c0a2fb065cba3a015b13#body_text
is schema:about of	named entity 'GOOSE PARVOVIRUS' named entity 'NOVEL' named entity 'REPEATABILITY' named entity 'COULD BE' named entity 'SET' named entity 'CONFIRMED BY' named entity 'NOVEL' named entity 'MARCH' named entity 'INFECTIOUS DISEASE' named entity 'INDICATED' named entity 'QUANTITATIVE ANALYSIS' named entity 'RAPID' named entity 'SENSITIVE' named entity 'INTRA' named entity 'STUDY' named entity 'TONGUES' named entity 'ATTACKED' named entity 'WAS A' named entity 'COMPLETED' named entity 'INDUSTRY' named entity 'PARVOVIRUS' named entity 'DUCK' named entity 'PROTRUDING ' named entity '2.25' named entity 'REAL-TIME PCR ' named entity 'SHARED' named entity 'ECONOMIC' named entity 'IDENTIFICATION' named entity 'SEQUENCE ANALYSIS' named entity 'IDENTITY' named entity 'VARIABILITY' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'DEVELOPMENT' named entity 'OUTER' named entity 'TESTS' named entity 'ORIGIN' named entity 'INNER' named entity 'GOOSE PARVOVIRUS' named entity 'ISOLATION' named entity 'PRIMERS' named entity 'POOR GROWTH' named entity '2015' named entity 'CROSS' named entity 'GENE' named entity 'DETECTION LIMIT' named entity 'DETECTING' named entity 'TARGETING' named entity 'HAVE' named entity 'PCR INSTRUMENT' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'FIELD' named entity 'PATHOGENS' named entity 'QUANTITATIVE' named entity 'DETECTION' named entity 'ISOTHERMAL AMPLIFICATION ASSAY' named entity 'CAUSED' named entity 'NUCLEOTIDE' named entity 'GPV' named entity 'MEAT' named entity 'RAPID DIAGNOSIS' named entity 'DUCKS' named entity 'GENOMIC SEQUENCE' named entity 'SHORT' named entity 'CHARACTERIZED' named entity 'ASSAY' named entity 'PATHOGEN' named entity 'WATER BATH' named entity 'SIMPLE' named entity 'VP3' named entity 'METHOD' named entity 'RAPID' named entity 'LAMP' named entity 'ACCURATE'

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