About: Broad Cross-Species Infection of Cultured Cells by Bat HKU2-Related Swine Acute Diarrhea Syndrome Coronavirus and Identification of Its Replication in Murine Dendritic Cells In Vivo Highlight Its Potential for Diverse Interspecies Transmission

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About: Broad Cross-Species Infection of Cultured Cells by Bat HKU2-Related Swine Acute Diarrhea Syndrome Coronavirus and Identification of Its Replication in Murine Dendritic Cells In Vivo Highlight Its Potential for Diverse Interspecies Transmission Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Broad Cross-Species Infection of Cultured Cells by Bat HKU2-Related Swine Acute Diarrhea Syndrome Coronavirus and Identification of Its Replication in Murine Dendritic Cells In Vivo Highlight Its Potential for Diverse Interspecies Transmission
Creator	Zhou, Jiyong Wang, Bin Liu, Yan Zhu, Shu Huang, Yao-Wei Qin, Pan Yang, Yong-Le Peng, Lei Xu, Guo-Han Huang, Liu, Wang Sj, Zhu Yang, Citation
Source	Medline; PMC
abstract	Outbreaks of severe diarrhea in neonatal piglets in Guangdong, China, in 2017 resulted in the isolation and discovery of a novel swine enteric alphacoronavirus (SeACoV) derived from the species Rhinolophus bat coronavirus HKU2 (Y. Pan, X. Tian, P. Qin, B. Wang, et al., Vet Microbiol 211:15–21, 2017). SeACoV was later referred to as swine acute diarrhea syndrome CoV (SADS-CoV) by another group (P. Zhou, H. Fan, T. Lan, X.-L. Yang, et al., Nature 556:255–258, 2018). The present study was set up to investigate the potential species barriers of SADS-CoV in vitro and in vivo. We first demonstrated that SADS-CoV possesses a broad species tropism and is able to infect cell lines from diverse species, including bats, mice, rats, gerbils, hamsters, pigs, chickens, nonhuman primates, and humans. Trypsin contributes to but is not essential for SADS-CoV propagation in vitro. Furthermore, C57BL/6J mice were inoculated with the virus via oral or intraperitoneal routes. Although the mice exhibited only subclinical infection, they supported viral replication and prolonged infection in the spleen. SADS-CoV nonstructural proteins and double-stranded RNA were detected in splenocytes of the marginal zone on the edge of lymphatic follicles, indicating active replication of SADS-CoV in the mouse model. We identified that splenic dendritic cells (DCs) are the major targets of virus infection by immunofluorescence and flow cytometry approaches. Finally, we demonstrated that SADS-CoV does not utilize known CoV receptors for cellular entry. The ability of SADS-CoV to replicate in various cells lines from a broad range of species and the unexpected tropism for murine DCs provide important insights into the biology of this bat-origin CoV, highlighting its possible ability to cross interspecies barriers. IMPORTANCE Infections with bat-origin coronaviruses (CoVs) (severe acute respiratory syndrome CoV [SARS-CoV] and Middle East respiratory syndrome CoV [MERS-CoV]) have caused severe illness in humans after “host jump” events. Recently, a novel bat-HKU2-like CoV named swine acute diarrhea syndrome CoV (SADS-CoV) has emerged in southern China, causing lethal diarrhea in newborn piglets. It is important to assess the species barriers of SADS-CoV infection since the animal hosts (other than pigs and bats) and zoonotic potential are still unknown. An in vitro susceptibility study revealed a broad species tropism of SADS-CoV, including various rodent and human cell lines. We established a mouse model of SADS-CoV infection, identifying its active replication in splenic dendritic cells, which suggests that SADS-CoV has the potential to infect rodents. These findings highlight the potential cross-species transmissibility of SADS-CoV, although further surveillance in other animal populations is needed to fully understand the ecology of this bat-HKU2-origin CoV.
has issue date	2019-11-26(xsd:dateTime)
bibo:doi	10.1128/jvi.01448-19
bibo:pmid	31554686
has license	no-cc
sha1sum (hex)	284053d25cd426f6121c3fed5ea6a017717853ed
schema:url	https://doi.org/10.1128/jvi.01448-19
resource representing a document's title	Broad Cross-Species Infection of Cultured Cells by Bat HKU2-Related Swine Acute Diarrhea Syndrome Coronavirus and Identification of Its Replication in Murine Dendritic Cells In Vivo Highlight Its Potential for Diverse Interspecies Transmission
has PubMed Central identifier	PMC6880172
has PubMed identifier	31554686
schema:publication	J Virol
resource representing a document's body	covid:284053d25cd426f6121c3fed5ea6a017717853ed#body_text
is schema:about of	named entity 'SWINE ENTERIC ALPHACORONAVIRUS' named entity 'NONHUMAN PRIMATES' named entity 'IN VIVO' covid:arg/284053d25cd426f6121c3fed5ea6a017717853ed named entity 'mice' named entity 'subclinical infection' named entity 'neonatal' named entity 'C57BL/6J' named entity 'swine' named entity 'diarrhea' named entity 'marginal zone' named entity 'swine' named entity 'SADS-CoV' named entity 'nonhuman primates' named entity 'SADS-CoV' named entity 'SADS-CoV' named entity 'SADS-CoV' named entity 'flow cytometry' named entity 'China' named entity 'infection' named entity 'Dendritic Cells' named entity 'fluorescence-activated cell sorter' named entity 'trypsin' named entity 'gerbil' named entity 'SADS-CoV' named entity 'antigen' named entity 'Staining' named entity 'plasmids' named entity 'overexpressing' named entity 'cell surface markers' named entity 'DMEM' named entity 'virus' named entity 'hpi' named entity 'spleens' named entity 'qRT-PCR' named entity 'cell lines' named entity 'hematoxylin and eosin' named entity 'Alexa Fluor' named entity 'trypsin' named entity 'MDCK' named entity 'white pulp' named entity 'wild-type' named entity 'flow cytometry' named entity 'MDCK' named entity 'antibody' named entity 'intestine' named entity 'SADS-CoV' named entity 'Changchun' named entity 'MAb' named entity 'multiplicity of infection' named entity 'SADS-CoV' named entity 'flow cytometry' named entity 'Huh-7' named entity 'cell lines' named entity 'SADS-CoV' named entity 'SADS-CoV' named entity 'polyethylenimine' named entity 'antibodies' named entity 'MDCK' named entity 'intestine' named entity 'receptor' named entity 'MERS-CoV' named entity 'dsRNA' named entity 'polyclonal antibodies' named entity 'SADS-CoV' named entity 'quantitative RT-PCR' named entity 'antibodies' named entity 'Trizol' named entity 'DF-1'

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