About: The Vpu-interacting Protein SGTA Regulates Expression of a Non-glycosylated Tetherin Species

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About: The Vpu-interacting Protein SGTA Regulates Expression of a Non-glycosylated Tetherin Species Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The Vpu-interacting Protein SGTA Regulates Expression of a Non-glycosylated Tetherin Species
Creator	Swiderski, Maya Xu, Yue Freed, Eric Khan, Maisha Macdonald, Scott Mounts, Megan Waheed, Abdul Ye, Yihong
Source	Medline; PMC
abstract	The HIV-1 accessory protein Vpu enhances virus release by counteracting the host restriction factor tetherin. To further understand the role of host cell proteins in Vpu function, we carried out yeast two-hybrid screening and identified a previously reported Vpu-interacting host factor, small glutamine-rich tetratricopeptide repeat-containing protein (SGTA). While RNAi-mediated depletion of SGTA did not significantly affect levels of tetherin or virus release efficiency, we observed that overexpression of SGTA inhibited HIV-1 release in a Vpu- and tetherin-independent manner. Overexpression of SGTA in the presence of Vpu, but not in its absence, resulted in a marked stabilization and cytosolic relocalization of a 23-kDa, non-glycosylated tetherin species. Coimmunoprecipitation studies indicated that non-glycosylated tetherin is stabilized through the formation of a ternary SGTA/Vpu/tetherin complex. This accumulation of non-glycosylated tetherin is due to inhibition of its degradation, independent of the ER-associated degradation (ERAD) pathway. Because the SGTA-stabilized tetherin species is partially localized to the cytosol, we propose that overexpression of SGTA in the presence of Vpu blocks the translocation of tetherin across the ER membrane, resulting in cytosolic accumulation of a non-glycosylated tetherin species. Although our results do not provide support for a physiological function of SGTA in HIV-1 replication, they demonstrate that SGTA overexpression regulates tetherin expression and stability, thus providing insights into the function of SGTA in ER translocation and protein degradation.
has issue date	2016-04-22(xsd:dateTime)
bibo:doi	10.1038/srep24934
bibo:pmid	27103333
has license	cc-by
sha1sum (hex)	2a3a68a67776a01043a6a156b69be803c7118a6d
schema:url	https://doi.org/10.1038/srep24934
resource representing a document's title	The Vpu-interacting Protein SGTA Regulates Expression of a Non-glycosylated Tetherin Species
has PubMed Central identifier	PMC4840321
has PubMed identifier	27103333
schema:publication	Sci Rep
resource representing a document's body	covid:2a3a68a67776a01043a6a156b69be803c7118a6d#body_text
is schema:about of	named entity 'VPU' named entity 'formation' named entity 'expression' named entity 'release' named entity 'degradation' named entity 'Vpu' named entity 'demonstrate' named entity 'cytosolic' named entity 'support' named entity 'stabilized' named entity 'SGTA' named entity 'INDEPENDENT OF' named entity 'STABILIZATION' named entity 'ACCESSORY PROTEIN' named entity 'COMPLEX' named entity 'PROVIDING' named entity 'FACTOR' named entity 'INTERACTING' named entity 'NON-' named entity 'EXPRESSION' named entity 'STABILIZED' named entity 'LEVELS' named entity 'KDA' named entity 'OUR' named entity 'PARTIALLY' named entity 'PROTEIN ' named entity 'EXPRESSION' named entity 'HOST FACTOR' named entity 'IDENTIFIED' named entity 'STUDIES' named entity 'OVEREXPRESSION' named entity 'ROLE' named entity 'PRESENCE OF' named entity 'TETRATRICOPEPTIDE REPEAT' named entity 'ER-' named entity 'COIMMUNOPRECIPITATION' named entity 'HOST CELL' named entity 'PROVIDE' named entity 'SPECIES' named entity 'OPEN' named entity 'GLYCOSYLATED' named entity 'INDEPENDENT' named entity 'RELOCALIZATION' named entity 'SMALL' named entity 'DUE TO' named entity 'RESTRICTION' named entity 'LOCALIZED' named entity 'ER MEMBRANE' named entity 'TETHERIN' named entity 'PREVIOUSLY' named entity 'HIV-1 REPLICATION' named entity 'PHYSIOLOGICAL FUNCTION' named entity 'INTERACTING' named entity 'INHIBITION' named entity 'VIRUS RELEASE' named entity 'CARRIED' named entity 'ACCUMULATION' named entity 'TRANSLOCATION' named entity 'RESULTS' named entity 'SCREENING' named entity 'NON-' named entity 'STABILITY' named entity 'INSIGHTS' named entity 'BUT' named entity 'FORMATION' named entity 'PROTEIN DEGRADATION' named entity 'YEAST' named entity 'ACROSS' named entity 'PATHWAY' named entity 'MARKED' named entity 'CYTOSOLIC' named entity 'RELEASE' named entity 'EFFICIENCY' named entity 'PROTEINS'

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