About: Fish Autophagy Protein 5 Exerts Negative Regulation on Antiviral Immune Response Against Iridovirus and Nodavirus

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Fish Autophagy Protein 5 Exerts Negative Regulation on Antiviral Immune Response Against Iridovirus and Nodavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Fish Autophagy Protein 5 Exerts Negative Regulation on Antiviral Immune Response Against Iridovirus and Nodavirus
Creator	Zhang, Xin Huang, Xiaohong Huang, Youhua Qin, Qiwei Li, Chen Li, C Liu, Jiaxin Wei, Jingguang Wei, Shina Huang, Huang Liu, Hai-Peng Dewitte-Orr, Stephanie Lu, Yishan
Source	PMC
abstract	Autophagy is an important biological activity that maintains homeostasis in eukaryotic cells. However, little is known about the functions of fish autophagy-related genes (Atgs). In this study, we cloned and characterized Atg5, a key gene in the autophagy gene superfamily, from orange-spotted grouper (Epinephelus coioides) (EcAtg5). EcAtg5 encoded a 275-amino acid protein that shared 94 and 81% identity to seabass (Lates calcarifer) and humans (Homo sapiens), respectively. The transcription level of EcAtg5 was significantly increased in cells infected with red-spotted grouper nervous necrosis virus (RGNNV). In cells infected with Singapore grouper iridovirus (SGIV), EcAtg5 expression declined during the early stage of infection and increased in the late stage. Fluorescence microscopy revealed that EcAtg5 mainly localized with a dot-like pattern in the cytoplasm of grouper cells. Overexpression of EcAtg5 significantly increased the replication of RGNNV and SGIV at different levels of detection, as indicated by increased severity of the cytopathic effect, transcription levels of viral genes, and levels of viral proteins. Knockdown of EcAtg5 decreased the replication of RGNNV and SGIV. Further studies showed that overexpression EcAtg5 activated autophagy, decreased expression levels of interferon related cytokines or effectors and pro-inflammatory factors, and inhibited the activation of nuclear factor κB, IFN-sensitive response element, and IFNs. In addition, ectopic expression of EcAtg5 affected cell cycle progression by hindering the G1/S transition. Taken together, our results demonstrated that fish Atg5 exerted a crucial role in virus replication by promoting autophagy, down-regulating antiviral IFN responses, and affecting the cell cycle.
has issue date	2019-03-19(xsd:dateTime)
bibo:doi	10.3389/fimmu.2019.00517
bibo:pmid	30941145
has license	cc-by
sha1sum (hex)	2a8e26038c98efac0a61629aa6b768fefad5a573
schema:url	https://doi.org/10.3389/fimmu.2019.00517
resource representing a document's title	Fish Autophagy Protein 5 Exerts Negative Regulation on Antiviral Immune Response Against Iridovirus and Nodavirus
has PubMed Central identifier	PMC6433989
has PubMed identifier	30941145
schema:publication	Front Immunol
resource representing a document's body	covid:2a8e26038c98efac0a61629aa6b768fefad5a573#body_text
is schema:about of	named entity 'EXERTS' named entity 'genes' named entity 'gene' named entity 'However' named entity 'autophagy' named entity 'humans' named entity 'functions' named entity 'sapiens' named entity 'Antiviral' named entity 'IFN' named entity 'ENCODED' named entity 'CYTOPATHIC EFFECT' named entity 'HOMEOSTASIS' named entity 'AMINO ACID PROTEIN' named entity 'LATE STAGE' named entity 'TRANSITION' named entity 'IDENTITY' named entity 'REGULATING' named entity 'CELL CYCLE' named entity 'SEVERITY' named entity 'INFECTION' named entity 'LEVELS' named entity 'INHIBITED' named entity 'AUTOPHAGY' named entity 'RESPONSES' named entity 'CHARACTERIZED' named entity 'EARLY STAGE' named entity 'EXPRESSION' named entity 'INCREASED' named entity 'REPLICATION' named entity 'PRO-' named entity 'CLONED' named entity 'GROUPER' named entity 'LITTLE' named entity 'RESULTS' named entity 'ACTIVATED' named entity 'STUDIES' named entity 'FISH' named entity 'VIRAL PROTEINS' named entity 'CELLS' named entity 'AFFECTING' named entity 'LOCALIZED' named entity 'CELL CYCLE PROGRESSION' named entity 'ORANGE-SPOTTED GROUPER' named entity 'NEGATIVE REGULATION' named entity 'KNOWN' named entity 'DETECTION' named entity 'TAKEN' named entity 'REVEALED' named entity 'DEMONSTRATED' named entity 'RESPONSE ELEMENT' named entity 'HUMANS' named entity 'ATG5' named entity 'PATTERN' named entity 'LIKE' named entity 'OVEREXPRESSION' named entity 'BIOLOGICAL ACTIVITY' named entity 'ARTICLE' named entity 'ANTIVIRAL IMMUNE RESPONSE' named entity 'INFECTED' named entity 'AUTOPHAGY PROTEIN 5' named entity 'FISH' named entity 'IRIDOVIRUS' named entity 'AUTOPHAGY-RELATED GENES' named entity 'DIFFERENT' named entity 'PROMOTING' named entity 'LEVEL' named entity 'DOT' named entity 'EUKARYOTIC CELLS'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software