About: Polarized Entry of Human Parechoviruses in the Airway Epithelium

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About: Polarized Entry of Human Parechoviruses in the Airway Epithelium Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Polarized Entry of Human Parechoviruses in the Airway Epithelium
Creator	Koekkoek, Sylvie Pajkrt, Dasja Yu, Xiao De Jong, Menno Cristella, Cosimo Hulsdouw, Rens Karelehto, Eveliina Sridhar, Adithya Wolthers, Katja Campos, Samuel De Haan, Karen Gustin, Kurt Van Eijk, Hetty
topic	covid:2b2ec303d28afd9e460b710dd23efe264a5359f0#this
source	Medline; PMC
abstract	Human parechoviruses (HPeVs), a poorly studied genus within the Picornaviridae family, are classified into 19 genotypes of which HPeV1 and HPeV3 are the most often detected. HPeV1 VP1 C terminus contains an arginine-glycine-aspartic acid (RGD) motif and has been shown to depend on the host cell surface αV integrins (αV ITGs) and heparan sulfate (HS) for entry. HPeV3 lacks this motif and the receptors remain unknown. HPeVs can be detected in patient nasopharyngeal and stool samples, and infection is presumed to occur after respiratory or gastro-intestinal transmission. HPeV pathogenesis is poorly understood as there are no animal models and previous studies have been conducted in immortalized monolayer cell cultures which do not adequately represent the characteristics of human tissues. To bridge this gap, we determined the polarity of infection, replication kinetics, and cell tropism of HPeV1 and HPeV3 in the well-differentiated human airway epithelial (HAE) model. We found the HAE cultures to be permissive for HPeVs. Both HPeV genotypes infected the HAE preferentially from the basolateral surface while the progeny virus was shed toward the apical side. Confocal microscopy revealed the target cell type to be the p63(+) basal cells for both viruses, αV ITG and HS blocking had no effect on the replication of either virus, and transcriptional profiling suggested that HPeV3 infection induced stronger immune activation than HPeV1. Genotype-specific host responses may contribute to the differences in pathogenesis and clinical outcomes associated with HPeV1 and HPeV3.
has issue date	2018-08-22(xsd:dateTime)
bibo:doi	10.3389/fcimb.2018.00294
bibo:pmid	30211126
has license	cc-by
sha1sum (hex)	2b2ec303d28afd9e460b710dd23efe264a5359f0
schema:url	https://doi.org/10.3389/fcimb.2018.00294
resource representing a document's title	Polarized Entry of Human Parechoviruses in the Airway Epithelium
has PubMed Central identifier	PMC6119779
has PubMed identifier	30211126
schema:publication	Front Cell Infect Microbiol
resource representing a document's body	covid:2b2ec303d28afd9e460b710dd23efe264a5359f0#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/2b2ec303d28afd9e460b710dd23efe264a5359f0
is schema:about of	named entity 'TRANSCRIPTIONAL PROFILING' named entity 'REPLICATION' named entity 'HUMAN TISSUES' named entity 'HOST' named entity 'HEPARAN SULFATE' named entity 'PATIENT' named entity 'EPITHELIAL' named entity 'ANIMAL MODELS ' named entity 'NO EFFECT' named entity 'MOTIF' named entity 'VP1' named entity 'CONFOCAL MICROSCOPY' named entity 'GAP' named entity 'GENUS' named entity 'OCCUR' named entity 'HUMAN' named entity 'WELL-DIFFERENTIATED' named entity 'GASTRO-INTESTINAL' named entity 'HUMAN PARECHOVIRUSES' named entity 'POLARITY' named entity 'PRESUMED' named entity 'RECEPTORS' named entity 'FOUND' named entity 'INFECTION INDUCED' named entity 'STOOL' named entity 'CELL' named entity 'FAMILY' named entity 'acid' named entity 'basolateral' named entity 'activation' named entity 'remain' named entity 'conducted' named entity 'Confocal microscopy' named entity 'pathogenesis' named entity 'immune' named entity 'epithelial' named entity 'human tissues' covid:arg/2b2ec303d28afd9e460b710dd23efe264a5359f0 named entity 'RESPIRATORY' named entity 'SURFACE' named entity 'ARGININE' named entity 'SPECIFIC' named entity 'BASOLATERAL' named entity 'BLOCKING' named entity 'INTEGRINS' named entity 'HOST CELL SURFACE' named entity 'BASAL CELLS' named entity 'INFECTION' named entity 'DO NOT' named entity 'APICAL SIDE' named entity 'PATHOGENESIS' named entity 'SAMPLES' named entity 'STUDIES' named entity 'CULTURES' named entity 'HUMAN PARECHOVIRUSES' named entity 'ENTRY' named entity 'AIRWAY EPITHELIUM' named entity 'DETERMINED' named entity 'CONTRIBUTE ' named entity 'MODEL' named entity 'MONOLAYER CELL CULTURES' named entity 'GLYCINE' named entity 'CHARACTERISTICS' named entity 'NASOPHARYNGEAL' named entity 'CLASSIFIED' named entity 'TRANSMISSION' named entity 'RESPONSES'

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