About: Expression of Hemagglutinin/Esterase by a Mouse Hepatitis Virus Coronavirus Defective–Interfering RNA Alters Viral Pathogenesis

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About: Expression of Hemagglutinin/Esterase by a Mouse Hepatitis Virus Coronavirus Defective–Interfering RNA Alters Viral Pathogenesis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Expression of Hemagglutinin/Esterase by a Mouse Hepatitis Virus Coronavirus Defective–Interfering RNA Alters Viral Pathogenesis
Creator	Lai, Michael Liao, Ching-Len Zhang, Xuming Park, Sungmin Parra, Beatriz Hinton, David Stohlman, Stephen
source	Elsevier; Medline; PMC
abstract	Abstract A defective-interfering (DI) RNA of mouse hepatitis virus (MHV) was developed as a vector for expressing MHV hemagglutinin/esterase (HE) protein. The virus containing an expressed HE protein (A59-DE-HE) was generated by infecting cells with MHV-A59, which does not express HE, and transfecting thein vitro-transcribed DI RNA containing the HE gene. A similar virus (A59-DE-CAT) expressing the chloramphenicol acetyltransferase (CAT) was used as a control. These viruses were inoculated intracerebrally into mice, and the role of the HE protein in viral pathogenesis was evaluated. Results showed that all mice infected with parental A59 or A59-DE-CAT succumbed to infection by 9 days postinfection (p.i.), demonstrating that inclusion of the DI did not by itself alter pathogenesis. In contrast, 60% of mice infected with A59-DE-HE survived infection. HE- or CAT-specific subgenomic mRNAs were detected in the brains at days 1 and 2 p.i. but not later, indicating that the genes in the DI vector were expressed only in the early stage of viral infection. No significant difference in virus titer or viral antigen expression in brains was observed between A59-DE-HE- and A59-DE-CAT-infected mice, suggesting that virus replication in brain was not affected by the expression of HE. However, at day 3 p.i. there was a slight increase in the extent of inflammatory cell infiltration in the brains of the A59-DE-HE-infected mice. Surprisingly, virus titers in the livers of A59-DE-HE-infected mice were 3 log10lower than that of the A59-DE-CAT-infected mice at day 6 p.i. Also, substantially less necrosis and viral antigen were detected in the livers of the A59-DE-HE-infected mice. This may account for the reduced mortality of these mice. The possible contribution of the host immune system to this difference in pathogenesis was analyzed by comparing the expression of four cytokines. Results showed that both tumor necrosis factor-α and interleukin-6 mRNAs increased in the brains of the A59-DE-HE-infected mice at day 2 p.i., whereas interferon-γ and interleukin-1α mRNAs were similar between A59-DE-HE- and A59-DE-CAT-infected mice. These data suggest that the transient expression of HE protein enhances an early innate immune response, possibly contributing to the eventual clearance of virus from the liver. This study indicates the feasibility of the DI expression system for studying roles of viral proteins during MHV infection.
has issue date	1998-03-01(xsd:dateTime)
bibo:doi	10.1006/viro.1997.8993
bibo:pmid	9501044
has license	els-covid
sha1sum (hex)	2b3f7bd146b0afe15fc0c40bbd1b3eec4329d2e5
schema:url	https://doi.org/10.1006/viro.1997.8993
resource representing a document's title	Expression of Hemagglutinin/Esterase by a Mouse Hepatitis Virus Coronavirus Defective–Interfering RNA Alters Viral Pathogenesis
has PubMed Central identifier	PMC7131006
has PubMed identifier	9501044
schema:publication	Virology
resource representing a document's body	covid:2b3f7bd146b0afe15fc0c40bbd1b3eec4329d2e5#body_text
is schema:about of	named entity 'mice' named entity 'gene' named entity 'innate immune response' named entity 'vector' named entity 'mouse' named entity 'host' named entity 'viral' named entity 'viral pathogenesis' named entity 'chloramphenicol acetyltransferase' named entity 'expressing' named entity 'protein' named entity 'mice' named entity 'mice' named entity 'contrast' named entity 'tumor necrosis factor' named entity 'A59' named entity 'infected' named entity 'Esterase' named entity 'Expression' covid:arg/2b3f7bd146b0afe15fc0c40bbd1b3eec4329d2e5 named entity 'MHV' named entity 'infection' named entity 'immune system' named entity 'brain' named entity 'However' named entity 'expression' named entity 'early' named entity 'A59' named entity 'stage' named entity 'expressing' named entity 'mice' named entity 'slight' named entity 'reduced' named entity 'protein' named entity 'brains' named entity 'CAT' named entity 'A59' named entity 'livers' named entity 'MHV' named entity 'pathogenesis' named entity 'antigen' named entity 'significant difference' named entity 'interleukin-6' named entity 'viral pathogenesis' named entity 'antigen' named entity 'chloramphenicol acetyltransferase' named entity 'virus' named entity 'protein' named entity 'liver' named entity 'RNA' named entity 'mRNAs' named entity 'mice' named entity 'Hemagglutinin' named entity 'Esterase' named entity 'Mouse Hepatitis Virus' named entity 'This' named entity 'liver' named entity 'mice' named entity 'DTT' named entity 'intergenic' named entity 'MHV' named entity 'inflammatory cell' named entity 'gene' named entity 'protein' named entity 'specific immunity' named entity 'mRNA' named entity 'hemagglutination' named entity 'infection' named entity 'serum' named entity 'mononuclear cell' named entity 'virus infection' named entity 'gene' named entity 'helper virus' named entity 'viral pathogenesis' named entity 'titer'

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